| Tag | Content |
|---|
| CPLM ID | CPLM-012754 |
UniProt Accession | |
Genbank Protein ID | |
Genbank Nucleotide ID | |
Protein Name | 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 4 |
Protein Synonyms/Alias | 6PF-2-K/Fru-2,6-P2ase 4; PFK/FBPase 4; 6PF-2-K/Fru-2,6-P2ase testis-type isozyme; 6-phosphofructo-2-kinase; Fructose-2,6-bisphosphatase |
Gene Name | PFKFB4 |
Gene Synonyms/Alias | |
Created Date | July 27, 2013 |
Organism | Homo sapiens (Human) |
NCBI Taxa ID | 9606 |
Lysine Modification | Position | Peptide | Type | References |
|---|
| 104 | EEGLKIRKQCALAAL | ubiquitination | [1] | | 265 | GESELNLKGRIGGDP | ubiquitination | [1] | | 295 | FISDQNIKDLKVWTS | ubiquitination | [1] | | 305 | KVWTSQMKRTIQTAE | ubiquitination | [1] | | 362 | KYRYRYPKGESYEDL | ubiquitination | [1] |
|
Reference | [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments. Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA. Mol Cell Proteomics. 2013 Mar;12(3):825-31. [ PMID: 23266961] |
Functional Description | Synthesis and degradation of fructose 2,6-bisphosphate. |
Sequence Annotation | NP_BIND 46 53 ATP (By similarity).
REGION 1 249 6-phosphofructo-2-kinase.
REGION 250 469 Fructose-2,6-bisphosphatase.
ACT_SITE 129 129 Potential.
ACT_SITE 159 159 Potential.
ACT_SITE 257 257 Tele-phosphohistidine intermediate (By
ACT_SITE 326 326 Potential.
ACT_SITE 391 391 Proton donor (By similarity).
BINDING 103 103 Fructose-6-phosphate (By similarity).
BINDING 194 194 Fructose-6-phosphate (By similarity).
MOD_RES 444 444 Phosphothreonine; by PKC (Potential). |
Keyword | ATP-binding; Complete proteome; Hydrolase; Kinase; Multifunctional enzyme; Nucleotide-binding; Phosphoprotein; Polymorphism; Reference proteome; Transferase. |
Sequence Source | UniProt (SWISSPROT/TrEMBL); GenBank; EMBL |
Protein Length | 469 AA |
Protein Sequence | MASPRELTQN PLKKIWMPYS NGRPALHACQ RGVCMTNCPT LIVMVGLPAR GKTYISKKLT 60
RYLNWIGVPT REFNVGQYRR DVVKTYKSFE FFLPDNEEGL KIRKQCALAA LRDVRRFLSE 120
EGGHVAVFDA TNTTRERRAT IFNFGEQNGY KTFFVESICV DPEVIAANIV QVKLGSPDYV 180
NRDSDEATED FMRRIECYEN SYESLDEDLD RDLSYIKIMD VGQSYVVNRV ADHIQSRIVY 240
YLMNIHVTPR SIYLCRHGES ELNLKGRIGG DPGLSPRGRE FAKSLAQFIS DQNIKDLKVW 300
TSQMKRTIQT AEALGVPYEQ WKVLNEIDAG VCEEMTYEEI QDNYPLEFAL RDQDKYRYRY 360
PKGESYEDLV QRLEPVIMEL ERQENVLVIC HQAVMRCLLA YFLDKAAEQL PYLKCPLHTV 420
LKLTPVAYGC KVESIFLNVA AVNTHRDRPQ NVDISRPPEE ALVTVPAHQ 469 |
Gene Ontology | GO:0005829; C:cytosol; TAS:Reactome. GO:0003873; F:6-phosphofructo-2-kinase activity; NAS:UniProtKB. GO:0005524; F:ATP binding; IEA:UniProtKB-KW. GO:0004331; F:fructose-2,6-bisphosphate 2-phosphatase activity; IEA:EC. GO:0006003; P:fructose 2,6-bisphosphate metabolic process; NAS:UniProtKB. GO:0006000; P:fructose metabolic process; IEA:InterPro. GO:0006096; P:glycolysis; TAS:Reactome. GO:0044281; P:small molecule metabolic process; TAS:Reactome. |
Interpro | |
Pfam | |
SMART | |
PROSITE | |
PRINTS | |