UniProt Accession

 ASNA_ECOLI; P00963; Q2M862 

Genbank Protein ID

 V00263; K00826; J01657; L10328; U00096; AP009048; M10679 

Genbank Nucleotide ID

 CAA23512.1; AAA24253.1; AAA24248.1; AAA62096.1; AAC76767.1; BAE77544.1; AAA24249.1 

Protein Name

 Aspartate--ammonia ligase 

Protein Synonyms/Alias

 Asparagine synthetase A 

Gene Name

 asnA 

Gene Synonyms/Alias

 b3744; JW3722 

Created Date

 July 27, 2013 

Organism

 Escherichia coli (strain K12) 

NCBI Taxa ID

 83333 

Lysine Modification

Position	Peptide	Type	References
8	MKTAYIAKQRQISFV	acetylation	[1]
53	DNLSGCEKAVQVKVK	acetylation	[1]
75	EVVHSLAKWKRQTLG	acetylation	[1]
204	FLVGIGGKLSDGHRH	acetylation	[1]
262	RVDADTLKHQLALTG	acetylation	[1]

Reference

 [1] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618] 

Functional Description

 May amidate Asp of the extracellular death factor precursor Asn-Asn-Trp-Asp-Asn to generate Asn-Asn-Trp-Asn-Asn (Probable). 

Sequence Annotation

  

Keyword

 3D-structure; Amino-acid biosynthesis; Asparagine biosynthesis; ATP-binding; Complete proteome; Cytoplasm; Direct protein sequencing; Ligase; Nucleotide-binding; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 330 AA 

Protein Sequence

Gene Ontology

 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0004812; F:aminoacyl-tRNA ligase activity; IEA:InterPro.
 GO:0004071; F:aspartate-ammonia ligase activity; IDA:EcoCyc.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0006529; P:asparagine biosynthetic process; IDA:EcoCyc.
 GO:0070981; P:L-asparagine biosynthetic process; IEA:UniProtKB-UniPathway.
 GO:0006974; P:response to DNA damage stimulus; IEP:EcoliWiki.
 GO:0006418; P:tRNA aminoacylation for protein translation; IEA:InterPro. 

Interpro

 IPR006195; aa-tRNA-synth_II.
 IPR004618; AsnA. 

Pfam

 PF03590; AsnA 

SMART

  

PROSITE

 PS50862; AA_TRNA_LIGASE_II 

PRINTS