UniProt Accession

 DHAK_HUMAN; Q3LXA3; Q53EQ9; Q9BVA7; Q9H895 

Genbank Protein ID

 DQ138290; AK023915; AK223580; AP003108; BC001341 

Genbank Nucleotide ID

 ABA10576.1; BAB14722.1; BAD97300.1; AAH01341.1 

Protein Name

 Bifunctional ATP-dependent dihydroxyacetone kinase/FAD-AMP lyase (cyclizing) 

Protein Synonyms/Alias

 ATP-dependent dihydroxyacetone kinase; DHA kinase; Glycerone kinase; Triokinase; Triose kinase; FAD-AMP lyase (cyclizing); FAD-AMP lyase (cyclic FMN forming); FMN cyclase 

Gene Name

 DAK 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
46	RSDLDSLKGRVALLS	ubiquitination	[1]
372	AAGGSASKRMALVLE	ubiquitination	[1, 2, 3]
420	RAIQEWLKEGPPPAS	ubiquitination	[1]
465	AAQPLKAKTSLPAWS	ubiquitination	[1]
484	AGLEAMQKYGKAAPG	ubiquitination	[1]
522	DLLQVLTKAVKSAEA	ubiquitination	[1, 2, 4]

Reference

 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [4] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473] 

Functional Description

 Catalyzes both the phosphorylation of dihydroxyacetone and of glyceraldehyde, and the splitting of ribonucleoside diphosphate-X compounds among which FAD is the best substrate. 

Sequence Annotation

 DOMAIN 9 336 DhaK.
 DOMAIN 372 571 DhaL.
 NP_BIND 401 404 ATP (By similarity).
 NP_BIND 446 447 ATP (By similarity).
 NP_BIND 494 495 ATP (By similarity).
 NP_BIND 556 558 ATP (By similarity).
 REGION 56 59 Dihydroxyacetone binding (By similarity).
 ACT_SITE 221 221 Tele-hemiaminal-histidine intermediate
 BINDING 109 109 Dihydroxyacetone (By similarity).
 BINDING 114 114 Dihydroxyacetone (By similarity).
 BINDING 486 486 ATP; via carbonyl oxygen (By similarity).  

Keyword

 ATP-binding; Cobalt; Complete proteome; FAD; Kinase; Lyase; Magnesium; Manganese; Metal-binding; Multifunctional enzyme; Nucleotide-binding; Polymorphism; Reference proteome; Transferase. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 575 AA 

Protein Sequence

Gene Ontology

 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0034012; F:FAD-AMP lyase (cyclizing) activity; IEA:EC.
 GO:0004371; F:glycerone kinase activity; IEA:EC.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0050354; F:triokinase activity; IEA:EC.
 GO:0006071; P:glycerol metabolic process; IEA:InterPro.
 GO:0045087; P:innate immune response; TAS:Reactome. 

Interpro

 IPR004006; Dak1.
 IPR004007; Dak2.
 IPR012734; DhaK_ATP. 

Pfam

 PF02733; Dak1
 PF02734; Dak2 

SMART

  

PROSITE

 PS51481; DHAK
 PS51480; DHAL 

PRINTS