CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-023566
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Rac GTPase-activating protein 1 
Protein Synonyms/Alias
 Male germ cell RacGap; MgcRacGAP 
Gene Name
 Racgap1 
Gene Synonyms/Alias
 Mgcracgap 
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
249SWTRSRGKSGPLQPVacetylation[1]
Reference
 [1] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
 Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
 Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337] 
Functional Description
 Component of the centralspindlin complex that serves as a microtubule-dependent and Rho-mediated signaling required for the myosin contractile ring formation during the cell cycle cytokinesis. Plays key roles in controlling cell growth and differentiation of hematopoietic cells through mechanisms other than regulating Rac GTPase activity. Also involved in the regulation of growth-related processes in adipocytes and myoblasts. May be involved in regulating spermatogenesis and in the RACGAP1 pathway in neuronal proliferation. Shows strong GAP (GTPase activation) activity towards CDC42 and RAC1 and less towards RHOA. Essential for the early stages of embryogenesis. May play a role in regulating cortical activity through RHOA during cytokinesis. May participate in the regulation of sulfate transport in male germ cells. 
Sequence Annotation
 DOMAIN 350 540 Rho-GAP.
 ZN_FING 287 336 Phorbol-ester/DAG-type.
 REGION 107 286 Interaction with SLC26A8 (By similarity).
 MOD_RES 150 150 Phosphoserine; by PLK1 (By similarity).
 MOD_RES 155 155 Phosphoserine (By similarity).
 MOD_RES 158 158 Phosphoserine; by PLK1 (By similarity).
 MOD_RES 162 162 Phosphothreonine (By similarity).
 MOD_RES 165 165 Phosphoserine; by PLK1 (By similarity).
 MOD_RES 171 171 Phosphoserine; by PLK1 (By similarity).
 MOD_RES 204 204 Phosphoserine (By similarity).
 MOD_RES 207 207 Phosphoserine (By similarity).
 MOD_RES 215 215 Phosphoserine (By similarity).
 MOD_RES 258 258 Phosphoserine (By similarity).
 MOD_RES 343 343 Phosphothreonine (By similarity).
 MOD_RES 388 388 Phosphoserine; by AURKB (By similarity).
 MOD_RES 411 411 Phosphoserine; by AURKB (By similarity).
 MOD_RES 564 564 Phosphothreonine (By similarity).
 MOD_RES 577 577 Phosphothreonine (By similarity).
 MOD_RES 585 585 Phosphothreonine (By similarity).
 MOD_RES 602 602 Phosphothreonine (By similarity).  
Keyword
 Cell cycle; Cell division; Coiled coil; Complete proteome; Cytoplasm; Cytoplasmic vesicle; Cytoskeleton; Developmental protein; Differentiation; GTPase activation; Ion transport; Metal-binding; Nucleus; Phosphoprotein; Reference proteome; Spermatogenesis; Transport; Zinc; Zinc-finger. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 628 AA 
Protein Sequence
MDTTMVNLWT LFEQLVRRME IINEGNESIE FIQVVKDFED FRKKYQRTNQ ELEKFKDLLL 60
KAETGRSALD VKLKHARNQV DVEIKRRQRA EAECAKLEQQ IQLIRDILMC DTSGSIQLSE 120
EQKSALAFLN RGQASSGHAG NNRLSTIDES GSILSDISFD KTDESLDWDS SLVKNFKMKK 180
REKRRSNSRQ FIDGPPGPVK KTCSIGSTVD QANESIVAKT TVTVPSDGGP IEAVSTIETL 240
PSWTRSRGKS GPLQPVNSDS ALNSRPLEPR TDTDNLGTPQ NTGGMRLHDF VSKTVIKPES 300
CVPCGKRIKF GKLSLKCRDC RLVSHPECRD RCPLPCIPPL VGTPVKIGEG MLADFVSQAS 360
PMIPAIVVSC VNEIEQRGLT EAGLYRISGC DRTVKELKEK FLKVKTVPLL SKVDDIHVIC 420
SLLKDFLRNL KEPLLTFWLS KAFMEAAEIT DEDNSTAAMY QAVSELPQAN RDTLAFLMIH 480
LQRVSQSPDT KMDIANLAKV FGPTIVAHTV PNPDPVTMFQ DIKRQLKVVE RLLSLPLEYW 540
NQFMMVDQEN IDSQRGNGNS TPRTPDVKVS LLGPVTTPEF QLVKTPLSSS LSQRLYNLSK 600
STPRFGNKSK SATNLGQQGK FFPAPYLK 628 
Gene Ontology
 GO:0001669; C:acrosomal vesicle; IEA:UniProtKB-SubCell.
 GO:0097149; C:centralspindlin complex; ISS:UniProtKB.
 GO:0032154; C:cleavage furrow; ISS:UniProtKB.
 GO:0030496; C:midbody; ISS:UniProtKB.
 GO:0005634; C:nucleus; ISS:UniProtKB.
 GO:0051233; C:spindle midzone; ISS:UniProtKB.
 GO:0043014; F:alpha-tubulin binding; ISS:UniProtKB.
 GO:0048487; F:beta-tubulin binding; ISS:UniProtKB.
 GO:0043015; F:gamma-tubulin binding; ISS:UniProtKB.
 GO:0005096; F:GTPase activator activity; ISS:UniProtKB.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0008017; F:microtubule binding; ISS:UniProtKB.
 GO:0000915; P:cytokinesis, actomyosin contractile ring assembly; ISS:UniProtKB.
 GO:0007108; P:cytokinesis, initiation of separation; ISS:UniProtKB.
 GO:0009790; P:embryo development; IMP:UniProtKB.
 GO:0035556; P:intracellular signal transduction; IEA:InterPro.
 GO:0007405; P:neuroblast proliferation; IEP:UniProtKB.
 GO:0032467; P:positive regulation of cytokinesis; ISS:UniProtKB.
 GO:0051988; P:regulation of attachment of spindle microtubules to kinetochore; ISS:UniProtKB.
 GO:0007283; P:spermatogenesis; ISS:UniProtKB.
 GO:0051256; P:spindle midzone assembly involved in mitosis; ISS:UniProtKB.
 GO:0008272; P:sulfate transport; IEA:Compara. 
Interpro
 IPR002219; Prot_Kinase_C-like_PE/DAG-bd.
 IPR008936; Rho_GTPase_activation_prot.
 IPR000198; RhoGAP_dom. 
Pfam
 PF00130; C1_1
 PF00620; RhoGAP 
SMART
 SM00109; C1
 SM00324; RhoGAP 
PROSITE
 PS50238; RHOGAP
 PS00479; ZF_DAG_PE_1
 PS50081; ZF_DAG_PE_2 
PRINTS