CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-011048
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Centromere protein C 1 
Protein Synonyms/Alias
 CENP-C 1; Centromere autoantigen C; Interphase centromere complex protein 7 
Gene Name
 CENPC1 
Gene Synonyms/Alias
 CENPC; ICEN7 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
10ASGLDHLKNGYRRRFubiquitination[1, 2]
119QAHEVHQKILATDVSubiquitination[1]
180VIPSSAQKRETYTFEubiquitination[3]
260EIQRQAKKSFSTLFLubiquitination[2]
271TLFLETVKRKSESSPubiquitination[2]
399VNNYRSTKYEMYSKNubiquitination[2]
507FSSESKNKLVPEEVTubiquitination[2, 3]
534PSDWWVVKSEESPVYsumoylation[4]
721KQSKVIPKNRIHHKLsumoylation[4]
727PKNRIHHKLVLPSNTubiquitination[1]
746RTKRTRLKPLEYWRGsumoylation[4]
746RTKRTRLKPLEYWRGubiquitination[1, 2, 3]
807CLDNDERKTNLMVNLubiquitination[1]
871TPFFSTGKLILGPQEubiquitination[3, 5]
Reference
 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [3] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [4] In vitro modification of human centromere protein CENP-C fragments by small ubiquitin-like modifier (SUMO) protein: definitive identification of the modification sites by tandem mass spectrometry analysis of the isopeptides.
 Chung TL, Hsiao HH, Yeh YY, Shia HL, Chen YL, Liang PH, Wang AH, Khoo KH, Shoei-Lung Li S.
 J Biol Chem. 2004 Sep 17;279(38):39653-62. [PMID: 15272016]
 [5] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302] 
Functional Description
 Component of the CENPA-NAC (nucleosome-associated) complex, a complex that plays a central role in assembly of kinetochore proteins, mitotic progression and chromosome segregation. The CENPA-NAC complex recruits the CENPA-CAD (nucleosome distal) complex and may be involved in incorporation of newly synthesized CENPA into centromeres. CENPC recruits DNA methylation and DNMT3B to both centromeric and pericentromeric satellite repeats and regulates the histone code in these regions. 
Sequence Annotation
 REGION 737 759 MIF2 homology domain II.
 REGION 890 943 MIF2 homology domain III.
 MOTIF 259 273 Nuclear localization signal (Potential).
 MOTIF 484 499 Nuclear localization signal (Potential).
 MOTIF 558 574 Nuclear localization signal (Potential).
 MOTIF 780 798 Nuclear localization signal (Potential).
 MOD_RES 96 96 Phosphoserine.
 MOD_RES 130 130 Phosphothreonine.
 MOD_RES 146 146 Phosphoserine.
 MOD_RES 183 183 Phosphothreonine.
 MOD_RES 189 189 Phosphoserine.
 MOD_RES 225 225 Phosphoserine.
 MOD_RES 261 261 Phosphoserine.
 MOD_RES 316 316 Phosphoserine.
 MOD_RES 439 439 Phosphoserine.
 MOD_RES 538 538 Phosphoserine.
 MOD_RES 709 709 Phosphoserine.
 MOD_RES 710 710 Phosphoserine.
 MOD_RES 734 734 Phosphothreonine.
 MOD_RES 763 763 Phosphoserine.
 MOD_RES 773 773 Phosphoserine.  
Keyword
 Cell cycle; Cell division; Centromere; Chromosome; Complete proteome; DNA-binding; Kinetochore; Mitosis; Nucleus; Phosphoprotein; Polymorphism; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 943 AA 
Protein Sequence
MAASGLDHLK NGYRRRFCRP SRARDINTEQ GQNVLEILQD CFEEKSLAND FSTNSTKSVP 60
NSTRKIKDTC IQSPSKECQK SHPKSVPVSS KKKEASLQFV VEPSEATNRS VQAHEVHQKI 120
LATDVSSKNT PDSKKISSRN INDHHSEADE EFYLSVGSPS VLLDAKTSVS QNVIPSSAQK 180
RETYTFENSV NMLPSSTEVS VKTKKRLNFD DKVMLKKIEI DNKVSDEEDK TSEGQERKPS 240
GSSQNRIRDS EYEIQRQAKK SFSTLFLETV KRKSESSPIV RHAATAPPHS CPPDDTKLIE 300
DEFIIDESDQ SFASRSWITI PRKAGSLKQR TISPAESTAL LQGRKSREKH HNILPKTLAN 360
DKHSHKPHPV ETSQPSDKTV LDTSYALIGE TVNNYRSTKY EMYSKNAEKP SRSKRTIKQK 420
QRRKFMAKPA EEQLDVGQSK DENIHTSHIT QDEFQRNSDR NMEEHEEMGN DCVSKKQMPP 480
VGSKKSSTRK DKEESKKKRF SSESKNKLVP EEVTSTVTKS RRISRRPSDW WVVKSEESPV 540
YSNSSVRNEL PMHHNSSRKS TKKTNQSSKN IRKKTIPLKR QKTATKGNQR VQKFLNAEGS 600
GGIVGHDEIS RCSLSEPLES DEADLAKKKN LDCSRSTRSS KNEDNIMTAQ NVPLKPQTSG 660
YTCNIPTESN LDSGEHKTSV LEESGPSRLN NNYLMSGKND VDDEEVHGSS DDSKQSKVIP 720
KNRIHHKLVL PSNTPNVRRT KRTRLKPLEY WRGERIDYQG RPSGGFVISG VLSPDTISSK 780
RKAKENIGKV NKKSNKKRIC LDNDERKTNL MVNLGIPLGD PLQPTRVKDP ETREIILMDL 840
VRPQDTYQFF VKHGELKVYK TLDTPFFSTG KLILGPQEEK GKQHVGQDIL VFYVNFGDLL 900
CTLHETPYIL STGDSFYVPS GNYYNIKNLR NEESVLLFTQ IKR 943 
Gene Ontology
 GO:0005721; C:centromeric heterochromatin; IDA:UniProtKB.
 GO:0000777; C:condensed chromosome kinetochore; IEA:UniProtKB-SubCell.
 GO:0000780; C:condensed nuclear chromosome, centromeric region; IDA:BHF-UCL.
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0000776; C:kinetochore; IDA:UniProtKB.
 GO:0003677; F:DNA binding; TAS:ProtInc.
 GO:0051301; P:cell division; IEA:UniProtKB-KW.
 GO:0007059; P:chromosome segregation; IMP:UniProtKB.
 GO:0051382; P:kinetochore assembly; IMP:UniProtKB.
 GO:0007067; P:mitosis; IEA:UniProtKB-KW.
 GO:0000278; P:mitotic cell cycle; IMP:UniProtKB. 
Interpro
 IPR025974; Mif2/CENP-C_cupin.
 IPR014710; RmlC-like_jellyroll.
 IPR011051; RmlC_Cupin. 
Pfam
 PF11699; Mif2 
SMART
  
PROSITE
  
PRINTS