CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-015324
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 BRCA1-associated ATM activator 1 
Protein Synonyms/Alias
 BRCA1-associated protein required for ATM activation protein 1 
Gene Name
 BRAT1 
Gene Synonyms/Alias
 BAAT1; C7orf27 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
31ADDTCLEKLLDWFKTubiquitination[1, 2]
197DWPACAQKIMDHVEEubiquitination[2]
213LCSAATPKVTQALNVubiquitination[2]
304PLALGILKLEHCPQAubiquitination[2, 3]
358VDTLLASKSSCAGLLubiquitination[2, 3, 4, 5]
469SSPTVLKKAFQATLRubiquitination[2]
483RWLLSSPKTPGCSDLubiquitination[1, 2, 4, 6]
507ELFPVLQKRLCHPCWubiquitination[1, 2, 3, 4, 6, 7, 8, 9]
729LLLFLRDKIASYSSLubiquitination[1, 2, 3, 4, 5, 6, 7, 9]
797ESSDHVEKSPQSLLQubiquitination[2]
Reference
 [1] Ubiquitin ligase substrate identification through quantitative proteomics at both the protein and peptide levels.
 Lee KA, Hammerle LP, Andrews PS, Stokes MP, Mustelin T, Silva JC, Black RA, Doedens JR.
 J Biol Chem. 2011 Dec 2;286(48):41530-8. [PMID: 21987572]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [4] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [5] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [6] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [7] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [8] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [9] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789
Functional Description
 Required for activation of ATM following ionizing radiation. May act by regulating dephosphorylation of ATM. 
Sequence Annotation
 REPEAT 495 531 HEAT 1.
 REPEAT 544 576 HEAT 2.
 MOD_RES 742 742 Phosphoserine.  
Keyword
 Alternative splicing; Complete proteome; Epilepsy; Nucleus; Phosphoprotein; Polymorphism; Reference proteome; Repeat. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 821 AA 
Protein Sequence
MDPECAQLLP ALCAVLVDPR QPVADDTCLE KLLDWFKTVT EGESSVVLLQ EHPCLVELLS 60
HVLKVQDLSS GVLSFSLRLA GTFAAQENCF QYLQQGELLP GLFGEPGPLG RATWAVPTVR 120
SGWIQGLRSL AQHPSALRFL ADHGAVDTIF SLQGDSSLFV ASAASQLLVH VLALSMRGGA 180
EGQPCLPGGD WPACAQKIMD HVEESLCSAA TPKVTQALNV LTTTFGRCQS PWTEALWVRL 240
SPRVACLLER DPIPAAHSFV DLLLCVARSP VFSSSDGSLW ETVARALSCL GPTHMGPLAL 300
GILKLEHCPQ ALRTQAFQVL LQPLACVLKA TVQAPGPPGL LDGTADDATT VDTLLASKSS 360
CAGLLCRTLA HLEELQPLPQ RPSPWPQASL LGATVTVLRL CDGSAAPASS VGGHLCGTLA 420
GCVRVQRAAL DFLGTLSQGT GPQELVTQAL AVLLECLESP GSSPTVLKKA FQATLRWLLS 480
SPKTPGCSDL GPLIPQFLRE LFPVLQKRLC HPCWEVRDSA LEFLTQLSRH WGGQADFRCA 540
LLASEVPQLA LQLLQDPESY VRASAVTAMG QLSSQGLHAP TSPEHAEARQ SLFLELLHIL 600
SVDSEGFPRR AVMQVFTEWL RDGHADAAQD TEQFVATVLQ AASRDLDWEV RAQGLELALV 660
FLGQTLGPPR THCPYAVALP EVAPAQPLTE ALRALCHVGL FDFAFCALFD CDRPVAQKSC 720
DLLLFLRDKI ASYSSLREAR GSPNTASAEA TLPRWRAGEQ AQPPGDQEPE AVLAMLRSLD 780
LEGLRSTLAE SSDHVEKSPQ SLLQDMLATG GFLQGDEADC Y 821 
Gene Ontology
 GO:0005634; C:nucleus; IDA:UniProtKB.
 GO:0010212; P:response to ionizing radiation; IMP:UniProtKB. 
Interpro
 IPR011989; ARM-like.
 IPR016024; ARM-type_fold.
 IPR000357; HEAT. 
Pfam
 PF02985; HEAT 
SMART
  
PROSITE
 PS50077; HEAT_REPEAT 
PRINTS