CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-002350
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 ATP-dependent RNA helicase DED1 
Protein Synonyms/Alias
 DEAD box protein 1 
Gene Name
 DED1 
Gene Synonyms/Alias
 SPP81; YOR204W 
Created Date
 July 27, 2013 
Organism
 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 
NCBI Taxa ID
 559292 
Lysine Modification
Position
Peptide
Type
References
92GGRWIDGKHVPAPRNacetylation[1]
158GLLLENIKLARFTKPubiquitination[2]
164IKLARFTKPTPVQKYacetylation[1]
170TKPTPVQKYSVPIVAubiquitination[3]
246TQIFDEAKKFTYRSWacetylation[1]
530QEVPSFLKDAMMSAPubiquitination[3]
558NNNRDYRKAGGASAGubiquitination[3]
588SGWGSDSKSSGWGNSubiquitination[3]
Reference
 [1] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919]
 [2] Sites of ubiquitin attachment in Saccharomyces cerevisiae.
 Starita LM, Lo RS, Eng JK, von Haller PD, Fields S.
 Proteomics. 2012 Jan;12(2):236-40. [PMID: 22106047]
 [3] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301] 
Functional Description
 ATP-binding RNA helicase involved in translation initiation. Remodels RNA in response to ADP and ATP concentrations by facilitating disruption, but also formation of RNA duplexes. Has weak ATP-dependent affinity for dsRNA, but strong ATP- dependent affinity for ssRNA. Acts as a virus host factor involved in the replication of the MBV and the L-A viruses by promoting the negative-strand RNA synthesis. May be involved in recognition of the preinitiation complex and DNA binding of the RNA polymerase III and play a role in mRNA splicing. 
Sequence Annotation
 DOMAIN 173 362 Helicase ATP-binding.
 DOMAIN 373 533 Helicase C-terminal.
 NP_BIND 186 193 ATP.
 MOTIF 142 170 Q motif.
 MOTIF 306 309 DEAD box.
 MOD_RES 215 215 Phosphoserine.
 MOD_RES 218 218 Phosphoserine.
 MOD_RES 263 263 Phosphoserine.
 MOD_RES 535 535 Phosphoserine.
 MOD_RES 539 539 Phosphoserine.
 MOD_RES 543 543 Phosphoserine.
 MOD_RES 576 576 Phosphoserine.
 MOD_RES 598 598 Phosphoserine.  
Keyword
 ATP-binding; Complete proteome; Cytoplasm; Helicase; Hydrolase; Initiation factor; Nucleotide-binding; Phosphoprotein; Protein biosynthesis; Reference proteome; RNA-binding. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 604 AA 
Protein Sequence
MAELSEQVQN LSINDNNENG YVPPHLRGKP RSARNNSSNY NNNNGGYNGG RGGGSFFSNN 60
RRGGYGNGGF FGGNNGGSRS NGRSGGRWID GKHVPAPRNE KAEIAIFGVP EDPNFQSSGI 120
NFDNYDDIPV DASGKDVPEP ITEFTSPPLD GLLLENIKLA RFTKPTPVQK YSVPIVANGR 180
DLMACAQTGS GKTGGFLFPV LSESFKTGPS PQPESQGSFY QRKAYPTAVI MAPTRELATQ 240
IFDEAKKFTY RSWVKACVVY GGSPIGNQLR EIERGCDLLV ATPGRLNDLL ERGKISLANV 300
KYLVLDEADR MLDMGFEPQI RHIVEDCDMT PVGERQTLMF SATFPADIQH LARDFLSDYI 360
FLSVGRVGST SENITQKVLY VENQDKKSAL LDLLSASTDG LTLIFVETKR MADQLTDFLI 420
MQNFRATAIH GDRTQSERER ALAAFRSGAA TLLVATAVAA RGLDIPNVTH VINYDLPSDV 480
DDYVHRIGRT GRAGNTGLAT AFFNSENSNI VKGLHEILTE ANQEVPSFLK DAMMSAPGSR 540
SNSRRGGFGR NNNRDYRKAG GASAGGWGSS RSRDNSFRGG SGWGSDSKSS GWGNSGGSNN 600
SSWW 604 
Gene Ontology
 GO:0005737; C:cytoplasm; IC:SGD.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0004004; F:ATP-dependent RNA helicase activity; IDA:SGD.
 GO:0033592; F:RNA strand annealing activity; IDA:SGD.
 GO:0003743; F:translation initiation factor activity; IEA:UniProtKB-KW.
 GO:0006413; P:translational initiation; IMP:SGD. 
Interpro
 IPR011545; DNA/RNA_helicase_DEAD/DEAH_N.
 IPR014001; Helicase_ATP-bd.
 IPR001650; Helicase_C.
 IPR027417; P-loop_NTPase.
 IPR000629; RNA-helicase_DEAD-box_CS.
 IPR014014; RNA_helicase_DEAD_Q_motif. 
Pfam
 PF00270; DEAD
 PF00271; Helicase_C 
SMART
 SM00487; DEXDc
 SM00490; HELICc 
PROSITE
 PS00039; DEAD_ATP_HELICASE
 PS51192; HELICASE_ATP_BIND_1
 PS51194; HELICASE_CTER
 PS51195; Q_MOTIF 
PRINTS