UniProt Accession

 PSB7_YEAST; P30657; D6VTT3; E9P8Z8 

Genbank Protein ID

 X68663; D50617; AY692947; BK006940 

Genbank Nucleotide ID

 CAA48629.1; BAA09289.1; AAT92966.1; DAA12493.1 

Protein Name

 Proteasome subunit beta type-7 

Protein Synonyms/Alias

 Macropain subunit PRE4; Multicatalytic endopeptidase complex subunit PRE4; Proteasome component PRE4; Proteinase YSCE subunit PRE4 

Gene Name

 PRE4 

Gene Synonyms/Alias

 YFR050C 

Created Date

 July 27, 2013 

Organism

 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 

NCBI Taxa ID

 559292 

Lysine Modification

Position	Peptide	Type	References
243	NTGLTFKKNLQVENM	ubiquitination	[1]
251	NLQVENMKWDFAKDI	ubiquitination	[1]
259	WDFAKDIKGYGTQKI	ubiquitination	[1]

Reference

 [1] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301] 

Functional Description

 The proteasome degrades poly-ubiquitinated proteins in the cytoplasm and in the nucleus. It is essential for the regulated turnover of proteins and for the removal of misfolded proteins. The proteasome is a multicatalytic proteinase complex that is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. It has an ATP-dependent proteolytic activity. PRE3 and PRE4 are necessary for the peptidyl-glutamyl-peptide- hydrolyzing activity. 

Sequence Annotation

  

Keyword

 3D-structure; Complete proteome; Cytoplasm; Hydrolase; Nucleus; Protease; Proteasome; Reference proteome; Threonine protease. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 266 AA 

Protein Sequence

Gene Ontology

 GO:0005789; C:endoplasmic reticulum membrane; IC:SGD.
 GO:0005634; C:nucleus; IC:SGD.
 GO:0019774; C:proteasome core complex, beta-subunit complex; IDA:SGD.
 GO:0034515; C:proteasome storage granule; IDA:SGD.
 GO:0004298; F:threonine-type endopeptidase activity; IEA:UniProtKB-KW.
 GO:0043161; P:proteasomal ubiquitin-dependent protein catabolic process; IDA:SGD.
 GO:0010499; P:proteasomal ubiquitin-independent protein catabolic process; IDA:SGD.
 GO:0043248; P:proteasome assembly; IGI:SGD. 

Interpro

 IPR016050; Proteasome_bsu_CS.
 IPR016295; Proteasome_endopept_cplx_B.
 IPR001353; Proteasome_sua/b.
 IPR023333; Proteasome_suB-type. 

Pfam

 PF00227; Proteasome 

SMART

  

PROSITE

 PS00854; PROTEASOME_B_1
 PS51476; PROTEASOME_B_2 

PRINTS