UniProt Accession

 CIS3_YEAST; P47001; D6VW29 

Genbank Protein ID

 Z49433; AY693027; BK006943 

Genbank Nucleotide ID

 CAA89453.1; AAT93046.1; DAA08645.1 

Protein Name

 Cell wall mannoprotein CIS3 

Protein Synonyms/Alias

 Covalently-linked cell wall protein 5/11; Protein with internal repeats 4; Soluble cell wall protein 8 

Gene Name

 CIS3 

Gene Synonyms/Alias

 CCW11; CCW5; PIR4; SCW8; YJL158C; J0561 

Created Date

 July 27, 2013 

Organism

 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 

NCBI Taxa ID

 559292 

Lysine Modification

Position	Peptide	Type	References
110	KISSSASKTSTNATS	ubiquitination	[1]
126	SCATPSLKDSSCKNS	ubiquitination	[1]
149	DGVLTDAKGRIGSIV	ubiquitination	[1]

Reference

 [1] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301] 

Functional Description

 Component of the outer cell wall layer. Required for stability of the cell wall and for optimal growth. Required for resistance against several antifungal and cell wall-perturbing agents. 

Sequence Annotation

 REPEAT 65 78 PIR1/2/3.
 CARBOHYD 68 68 O-linked (Man).
 CARBOHYD 78 78 O-linked (Man).
 CARBOHYD 105 105 O-linked (Man).
 CARBOHYD 106 106 O-linked (Man).
 CARBOHYD 107 107 O-linked (Man).
 CARBOHYD 109 109 O-linked (Man).
 CARBOHYD 111 111 O-linked (Man).
 CARBOHYD 112 112 O-linked (Man).
 CARBOHYD 113 113 O-linked (Man).
 CARBOHYD 114 114 N-linked (GlcNAc...) (Potential).
 CARBOHYD 116 116 O-linked (Man).
 CARBOHYD 117 117 O-linked (Man).
 CARBOHYD 118 118 O-linked (Man).  

Keyword

 Cell wall; Cell wall biogenesis/degradation; Cleavage on pair of basic residues; Complete proteome; Direct protein sequencing; Glycoprotein; Reference proteome; Repeat; Secreted; Signal. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 227 AA 

Protein Sequence

Gene Ontology

 GO:0005934; C:cellular bud tip; IDA:SGD.
 GO:0005783; C:endoplasmic reticulum; IDA:SGD.
 GO:0005576; C:extracellular region; IDA:SGD.
 GO:0009277; C:fungal-type cell wall; IDA:SGD.
 GO:0005886; C:plasma membrane; IDA:SGD.
 GO:0005199; F:structural constituent of cell wall; ISS:SGD.
 GO:0031505; P:fungal-type cell wall organization; IMP:SGD. 

Interpro

 IPR000420; Yeast_PIR. 

Pfam

 PF00399; PIR 

SMART

  

PROSITE

 PS00929; PIR_REPEAT_1
 PS50256; PIR_REPEAT_2 

PRINTS