CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-000951
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Bifunctional protein NCOAT 
Protein Synonyms/Alias
 Meningioma-expressed antigen 5; Nuclear cytoplasmic O-GlcNAcase and acetyltransferase; Protein O-GlcNAcase; Glycoside hydrolase O-GlcNAcase; Hexosaminidase C; N-acetyl-beta-D-glucosaminidase; N-acetyl-beta-glucosaminidase; O-GlcNAcase; OGA; Histone acetyltransferase; HAT 
Gene Name
 MGEA5 
Gene Synonyms/Alias
 HEXC; KIAA0679; MEA5 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
240YLRTVGEKLLPGIEVubiquitination[1]
257TGPKVVSKEIPVESIubiquitination[1]
289HANDYDQKRLFLGPYubiquitination[1]
405LTKEEEKKQPDEEPMubiquitination[1]
418PMDMVVEKQEETDHKubiquitination[1]
Reference
 [1] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661] 
Functional Description
 Cleaves GlcNAc but not GalNAc from glycopeptides. Can use p-nitrophenyl-beta-GlcNAc as substrate but not p-nitrophenyl- beta-GalNAc or p-nitrophenyl-alpha-GlcNAc. Possesses hyaluronidase activity. Acetylates 'Lys-8' of histone H4 and 'Lys-14' of histone H3. 
Sequence Annotation
 REGION 583 916 Histone acetyltransferase activity (By
 REGION 695 814 Required for histone H4 binding (By
 ACT_SITE 175 175 Nucleophile; for O-GlcNAcase activity (By
 ACT_SITE 177 177 Proton donor; for O-GlcNAcase activity
 MOD_RES 364 364 Phosphoserine.
 DISULFID 777 793 By similarity.  
Keyword
 Acyltransferase; Alternative splicing; Complete proteome; Cytoplasm; Disulfide bond; Glycosidase; Hydrolase; Multifunctional enzyme; Nucleus; Phosphoprotein; Polymorphism; Reference proteome; Transferase. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 916 AA 
Protein Sequence
MVQKESQATL EERESELSSN PAASAGASLE PPAAPAPGED NPAGAGGAAV AGAAGGARRF 60
LCGVVEGFYG RPWVMEQRKE LFRRLQKWEL NTYLYAPKDD YKHRMFWREM YSVEEAEQLM 120
TLISAAREYE IEFIYAISPG LDITFSNPKE VSTLKRKLDQ VSQFGCRSFA LLFDDIDHNM 180
CAADKEVFSS FAHAQVSITN EIYQYLGEPE TFLFCPTEYC GTFCYPNVSQ SPYLRTVGEK 240
LLPGIEVLWT GPKVVSKEIP VESIEEVSKI IKRAPVIWDN IHANDYDQKR LFLGPYKGRS 300
TELIPRLKGV LTNPNCEFEA NYVAIHTLAT WYKSNMNGVR KDVVMSRQVA HSGAKASVVD 360
GTPLVAAPSL NATTVVTTVY QEPIMSQGAA LSGEPTTLTK EEEKKQPDEE PMDMVVEKQE 420
ETDHKNDNQI LSEIVEAKMA EELKPMDTDK ESIAESKSPE MSMQEDCISD IAPMQTDEQT 480
NKEQFVPGPN EKPLYTAEPV TLEDLQLLAD LFYLPYEHGP KGAQMLREFQ WLRANSSVVS 540
VNCKGKDSEK IEEWRSRAAK FEEMCGLVMG MFTRLSNCAN RTILYDMYSY VWDIKSIMSM 600
VKSFVQWLGC RSHSSAQFLI GDQEPWAFRG GLAGEFQPPP LTPTSKVYTI RPYFPKDEAS 660
VYKICREMYD DGVGLPFQSQ PDLIGDKLVG GLLSLSLDYC FVLEDEDGIC GYALGTVDVT 720
PFIKKCKISW IPFMQEKYTK PNGDKELSEA EKIMLSFHEE QEVLPETFLA NFPSLIKMDI 780
HKKVTDPSVA KSMMACLLSS LKANGSRGAF CEVRPDDKRI LEFYSKLGCF EIAKMEGFPK 840
DVVILGRSL 849 
Gene Ontology
 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
 GO:0004402; F:histone acetyltransferase activity; IEA:EC.
 GO:0004415; F:hyalurononglucosaminidase activity; TAS:ProtInc.
 GO:0007568; P:aging; IEA:Compara.
 GO:0046060; P:dATP metabolic process; IEA:Compara.
 GO:0006516; P:glycoprotein catabolic process; TAS:ProtInc.
 GO:0006044; P:N-acetylglucosamine metabolic process; IEA:Compara.
 GO:0070265; P:necrotic cell death; IEA:Compara.
 GO:0010616; P:negative regulation of cardiac muscle adaptation; IEA:Compara.
 GO:0060051; P:negative regulation of protein glycosylation; IEA:Compara.
 GO:0010524; P:positive regulation of calcium ion transport into cytosol; IEA:Compara.
 GO:0031343; P:positive regulation of cell killing; IEA:Compara.
 GO:0051054; P:positive regulation of DNA metabolic process; IEA:Compara.
 GO:0046326; P:positive regulation of glucose import; IEA:Compara.
 GO:0060124; P:positive regulation of growth hormone secretion; IEA:Compara.
 GO:0032024; P:positive regulation of insulin secretion; IEA:Compara.
 GO:0051901; P:positive regulation of mitochondrial depolarization; IEA:Compara.
 GO:0043243; P:positive regulation of protein complex disassembly; IEA:Compara.
 GO:0045862; P:positive regulation of proteolysis; IEA:Compara.
 GO:0006612; P:protein targeting to membrane; IEA:Compara.
 GO:0048545; P:response to steroid hormone stimulus; IEA:Compara. 
Interpro
 IPR016181; Acyl_CoA_acyltransferase.
 IPR011496; Beta-N-acetylglucosaminidase.
 IPR017853; Glycoside_hydrolase_SF. 
Pfam
 PF07555; NAGidase 
SMART
  
PROSITE
  
PRINTS