CPLM-011213

CPLM 1.0 - Compendium of Protein Lysine Modification

Tag

Content

CPLM ID

CPLM-011213

UniProt Accession

IF4G1_HUMAN; Q04637; D3DNT2; D3DNT4; D3DNT5; E9PFM1; G5E9S1; O43177; O95066; Q5HYG0; Q6ZN21; Q8N102

Genbank Protein ID

D12686; AY082886; AF281070; AK131407; BX647812; AC078797; CH471052; CH471052; CH471052; CH471052; CH471052; CH471052; CH471052; CH471052; AF002816; AF004836; AF104913; AJ001046

Genbank Nucleotide ID

BAA02185.1; AAL92872.1; AAM69365.1; BAD18554.1; CAI46013.1; EAW78257.1; EAW78259.1; EAW78262.1; EAW78263.1; EAW78264.1; EAW78265.1; EAW78266.1; EAW78267.1; AAC78443.1; AAC78444.1; AAC82471.1; CAA04500.1

Protein Name

Eukaryotic translation initiation factor 4 gamma 1

Protein Synonyms/Alias

eIF-4-gamma 1; eIF-4G 1; eIF-4G1; p220

Gene Name

EIF4G1

Gene Synonyms/Alias

EIF4F; EIF4G; EIF4GI

Created Date

July 27, 2013

Organism

Homo sapiens (Human)

NCBI Taxa ID

9606

Lysine Modification

Position	Peptide	Type	References
553	NIQPGEQKYEYKSDQ	ubiquitination	[1]
860	RRSLGNIKFIGELFK	ubiquitination	[1]

Reference

[1] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]

Functional Description

Component of the protein complex eIF4F, which is involved in the recognition of the mRNA cap, ATP-dependent unwinding of 5'-terminal secondary structure and recruitment of mRNA to the ribosome.

Sequence Annotation

DOMAIN 565 792 MIF4G.
DOMAIN 1241 1363 MI.
DOMAIN 1433 1599 W2.
REGION 172 200 PABPC1-binding.
REGION 607 618 EIF4E-binding.
REGION 682 1085 eIF3/EIF4A-binding.
REGION 1450 1599 EIF4A-binding.
REGION 1585 1599 Necessary but not sufficient for MKNK1-
MOD_RES 205 205 Phosphothreonine (By similarity).
MOD_RES 207 207 Phosphothreonine.
MOD_RES 223 223 Phosphothreonine.
MOD_RES 647 647 Phosphothreonine.
MOD_RES 1028 1028 Phosphoserine.
MOD_RES 1092 1092 Phosphoserine.
MOD_RES 1095 1095 N6-acetyllysine.
MOD_RES 1145 1145 Phosphoserine.
MOD_RES 1147 1147 Phosphoserine.
MOD_RES 1185 1185 Phosphoserine; by PKC/PRKCA.
MOD_RES 1187 1187 Phosphoserine.
MOD_RES 1209 1209 Phosphoserine.
MOD_RES 1211 1211 Phosphothreonine.
MOD_RES 1231 1231 Phosphoserine.
MOD_RES 1596 1596 Phosphoserine.

Keyword

3D-structure; Acetylation; Alternative initiation; Alternative splicing; Complete proteome; Disease mutation; Host-virus interaction; Initiation factor; Neurodegeneration; Parkinson disease; Parkinsonism; Phosphoprotein; Polymorphism; Protein biosynthesis; Reference proteome; RNA-binding; Translation regulation.

Sequence Source

UniProt (SWISSPROT/TrEMBL); GenBank; EMBL

Protein Length

1599 AA

Protein Sequence

MNTPSQPRQH FYPSRAQPPS SAASRVQSAA PARPGPAAHV YPAGSQVMMI PSQISYPASQ 60
GAYYIPGQGR STYVVPTQQY PVQPGAPGFY PGASPTEFGT YAGAYYPAQG VQQFPTGVAP 120
TPVLMNQPPQ IAPKRERKTI RIRDPNQGGK DITEEIMSGA RTASTPTPPQ TGGGLEPQAN 180
GETPQVAVIV RPDDRSQGAI IADRPGLPGP EHSPSESQPS SPSPTPSPSP VLEPGSEPNL 240
AVLSIPGDTM TTIQMSVEES TPISRETGEP YRLSPEPTPL AEPILEVEVT LSKPVPESEF 300
SSSPLQAPTP LASHTVEIHE PNGMVPSEDL EPEVESSPEL APPPACPSES PVPIAPTAQP 360
EELLNGAPSP PAVDLSPVSE PEEQAKEVTA SMAPPTIPSA TPATAPSATS PAQEEEMEEE 420
EEEEEGEAGE AGEAESEKGG EELLPPESTP IPANLSQNLE AAAATQVAVS VPKRRRKIKE 480
LNKKEAVGDL LDAFKEANPA VPEVENQPPA GSNPGPESEG SGVPPRPEEA DETWDSKEDK 540
IHNAENIQPG EQKYEYKSDQ WKPLNLEEKK RYDREFLLGF QFIFASMQKP EGLPHISDVV 600
LDKANKTPLR PLDPTRLQGI NCGPDFTPSF ANLGRTTLST RGPPRGGPGG ELPRGPAGLG 660
PRRSQQGPRK EPRKIIATVL MTEDIKLNKA EKAWKPSSKR TAADKDRGEE DADGSKTQDL 720
FRRVRSILNK LTPQMFQQLM KQVTQLAIDT EERLKGVIDL IFEKAISEPN FSVAYANMCR 780
CLMALKVPTT EKPTVTVNFR KLLLNRCQKE FEKDKDDDEV FEKKQKEMDE AATAEERGRL 840
KEELEEARDI ARRRSLGNIK FIGELFKLKM LTEAIMHDCV VKLLKNHDEE SLECLCRLLT 900
TIGKDLDFEK AKPRMDQYFN QMEKIIKEKK TSSRIRFMLQ DVLDLRGSNW VPRRGDQGPK 960
TIDQIHKEAE MEEHREHIKV QQLMAKGSDK RRGGPPGPPI SRGLPLVDDG GWNTVPISKG 1020
SRPIDTSRLT KITKPGSIDS NNQLFAPGGR LSWGKGSSGG SGAKPSDAAS EAARPATSTL 1080
NRFSALQQAV PTESTDNRRV VQRSSLSRER GEKAGDRGDR LERSERGGDR GDRLDRARTP 1140
ATKRSFSKEV EERSRERPSQ PEGLRKAASL TEDRDRGRDA VKREAALPPV SPLKAALSEE 1200
ELEKKSKAII EEYLHLNDMK EAVQCVQELA SPSLLFIFVR HGVESTLERS AIAREHMGQL 1260
LHQLLCAGHL STAQYYQGLY EILELAEDME IDIPHVWLYL AELVTPILQE GGVPMGELFR 1320
EITKPLRPLG KAASLLLEIL GLLCKSMGPK KVGTLWREAG LSWKEFLPEG QDIGAFVAEQ 1380
KVEYTLGEES EAPGQRALPS EELNRQLEKL LKEGSSNQRV FDWIEANLSE QQIVSNTLVR 1440
ALMTAVCYSA IIFETPLRVD VAVLKARAKL LQKYLCDEQK ELQALYALQA LVVTLEQPPN 1500
LLRMFFDALY DEDVVKEDAF YSWESSKDPA EQQGKGVALK SVTAFFKWLR EAEEESDHN 1559

Gene Ontology

GO:0005829; C:cytosol; TAS:Reactome.
GO:0016281; C:eukaryotic translation initiation factor 4F complex; TAS:ProtInc.
GO:0003677; F:DNA binding; IEA:InterPro.
GO:0003743; F:translation initiation factor activity; TAS:UniProtKB.
GO:0008219; P:cell death; IEA:UniProtKB-KW.
GO:0019221; P:cytokine-mediated signaling pathway; TAS:Reactome.
GO:0008286; P:insulin receptor signaling pathway; TAS:Reactome.
GO:0000184; P:nuclear-transcribed mRNA catabolic process, nonsense-mediated decay; TAS:Reactome.
GO:0000289; P:nuclear-transcribed mRNA poly(A) tail shortening; TAS:Reactome.
GO:0006446; P:regulation of translational initiation; IMP:UniProtKB.
GO:0019048; P:virus-host interaction; IEA:UniProtKB-KW.

Interpro

IPR016024; ARM-type_fold.
IPR003891; Initiation_fac_eIF4g_MI.
IPR016021; MIF4-like_typ_1/2/3.
IPR003890; MIF4G-like_typ-3.
IPR003307; W2_domain.

Pfam

PF02847; MA3
PF02854; MIF4G
PF02020; W2

SMART

SM00515; eIF5C
SM00544; MA3
SM00543; MIF4G

PROSITE

PS51366; MI
PS51363; W2

PRINTS