CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-011521
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Phosphatase PSR1 
Protein Synonyms/Alias
 Plasma membrane sodium response protein 1 
Gene Name
 PSR1 
Gene Synonyms/Alias
 YLL010C; L1341 
Created Date
 July 27, 2013 
Organism
 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 
NCBI Taxa ID
 559292 
Lysine Modification
Position
Peptide
Type
References
58QTNSPPSKTNSAATFubiquitination[1]
74STERSTGKSGISTNDubiquitination[1]
84ISTNDNEKKKPSSPTubiquitination[1, 2]
111KVEKRISKDDLYEEKubiquitination[1]
142SRGIVQEKGDAVKDTubiquitination[1]
154KDTSRQKKQQQQQQQubiquitination[1, 2]
Reference
 [1] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301]
 [2] Sites of ubiquitin attachment in Saccharomyces cerevisiae.
 Starita LM, Lo RS, Eng JK, von Haller PD, Fields S.
 Proteomics. 2012 Jan;12(2):236-40. [PMID: 22106047] 
Functional Description
 Has phosphatase activity in vitro. Involved in the response to sodium and lithium ion stress (but not to potassium or sorbitol stress) by inducing transcription of the sodium pump ENA1/PMR2. Acts through a calcineurin-independent pathway and is functionally redundant with PSR2. Also involved in the general stress response; acts together with WHI2 to activate stress response element (STRE)-mediated gene expression, possibly through dephosphorylation of MSN2. 
Sequence Annotation
 DOMAIN 253 411 FCP1 homology.
 MOD_RES 110 110 Phosphoserine.
 LIPID 9 9 S-palmitoyl cysteine (Potential).
 LIPID 10 10 S-palmitoyl cysteine (Potential).  
Keyword
 Cell membrane; Complete proteome; Hydrolase; Lipoprotein; Membrane; Palmitate; Phosphoprotein; Protein phosphatase; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 427 AA 
Protein Sequence
MGFISSILCC SSETTQSNSN SAYRQQQSSS LNKNRSVKHS NTKSRTRGVH QTNSPPSKTN 60
SAATFSSTER STGKSGISTN DNEKKKPSSP TAAVTATTTN NMTKVEKRIS KDDLYEEKYE 120
VDEDEEIDDE DNRRSRGIVQ EKGDAVKDTS RQKKQQQQQQ QQSQPQPQPQ SQSQSQSQSQ 180
SQQRGPTVQV SSDHLIQDMN LSRVSSSSQA SETSNDADDE DDEDEEYIDL TLLQQGQYHA 240
PGYNTLLPPQ DESTKGKKCL ILDLDETLVH SSFKYLRSAD FVLSVEIDDQ VHNVYVIKRP 300
GVEEFLERVG KLFEVVVFTA SVSRYGDPLL DILDTDKVIH HRLFREACYN YEGNYIKNLS 360
QIGRPLSDII ILDNSPASYI FHPQHAIPIS SWFSDTHDNE LLDIIPLLED LSVKTSLDVG 420
KILDVTI 427 
Gene Ontology
 GO:0005886; C:plasma membrane; IDA:SGD.
 GO:0004721; F:phosphoprotein phosphatase activity; IDA:SGD.
 GO:0009408; P:response to heat; IMP:UniProtKB.
 GO:0009651; P:response to salt stress; IMP:UniProtKB. 
Interpro
 IPR011948; Dullard_phosphatase.
 IPR023214; HAD-like_dom.
 IPR004274; NIF. 
Pfam
 PF03031; NIF 
SMART
 SM00577; CPDc 
PROSITE
 PS50969; FCP1 
PRINTS