UniProt Accession

 MEPCE_HUMAN; Q7L2J0; B3KP86; D6W5V7; Q9NPD4 

Genbank Protein ID

 AK055964; AC092849; CH471091; CH471091; CH471091; CH236956; BC000556; BC016396; BC018935; AF264752; AK000264 

Genbank Nucleotide ID

 BAG51598.1; EAW76534.1; EAW76535.1; EAW76536.1; EAL23834.1; AAH00556.2; AAH16396.1; AAH18935.2; AAF74767.1; BAA91040.1 

Protein Name

 7SK snRNA methylphosphate capping enzyme 

Protein Synonyms/Alias

 MePCE; Bicoid-interacting protein 3 homolog; Bin3 homolog 

Gene Name

 MEPCE 

Gene Synonyms/Alias

 BCDIN3 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
438	DGRLRVLKPEWFRGR	ubiquitination	[1]
635	TLTETIYKNYYRIQL	ubiquitination	[1, 2, 3]

Reference

 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [3] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789] 

Functional Description

 S-adenosyl-L-methionine-dependent methyltransferase that adds a methylphosphate cap at the 5'-end of 7SK snRNA, leading to stabilize it. 

Sequence Annotation

 DOMAIN 431 686 Bin3-type SAM.
 REGION 451 453 S-adenosyl-L-methionine binding.
 REGION 474 475 S-adenosyl-L-methionine binding.
 REGION 559 560 S-adenosyl-L-methionine binding.
 BINDING 581 581 S-adenosyl-L-methionine; via carbonyl
 MOD_RES 1 1 N-acetylmethionine.
 MOD_RES 57 57 Phosphoserine.
 MOD_RES 60 60 Phosphoserine.
 MOD_RES 69 69 Phosphoserine.
 MOD_RES 101 101 Phosphoserine.
 MOD_RES 152 152 Phosphoserine.
 MOD_RES 175 175 Phosphoserine.
 MOD_RES 179 179 Phosphoserine.
 MOD_RES 213 213 Phosphothreonine.
 MOD_RES 216 216 Phosphoserine.
 MOD_RES 217 217 Phosphoserine.
 MOD_RES 254 254 Phosphoserine.
 MOD_RES 330 330 Phosphoserine.
 MOD_RES 390 390 Phosphoserine.  

Keyword

 3D-structure; Acetylation; Alternative splicing; Complete proteome; Methyltransferase; Phosphoprotein; Reference proteome; S-adenosyl-L-methionine; Transferase. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 689 AA 

Protein Sequence

Gene Ontology

 GO:0008173; F:RNA methyltransferase activity; IDA:UniProtKB.
 GO:0008757; F:S-adenosylmethionine-dependent methyltransferase activity; IDA:UniProtKB.
 GO:0040031; P:snRNA modification; IDA:UniProtKB. 

Interpro

 IPR010675; Bin3.
 IPR024160; BIN3_SAM-bd_dom. 

Pfam

 PF06859; Bin3 

SMART

  

PROSITE

 PS51515; BIN3_SAM 

PRINTS