CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-022209
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Trimethyllysine dioxygenase, mitochondrial 
Protein Synonyms/Alias
 Epsilon-trimethyllysine 2-oxoglutarate dioxygenase; Epsilon-trimethyllysine hydroxylase; TML hydroxylase; TML-alpha-ketoglutarate dioxygenase; TML dioxygenase; TMLD 
Gene Name
 TMLHE 
Gene Synonyms/Alias
 TMLH 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
18SRLQDLLKGGVIYPAubiquitination[1]
135YDLNWLVKNSYEGQKubiquitination[1, 2, 3]
236RGDTAYTKLALDRHTacetylation[4]
236RGDTAYTKLALDRHTubiquitination[1, 5]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [3] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [4] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
 [5] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302] 
Functional Description
 Converts trimethyllysine (TML) into hydroxytrimethyllysine (HTML). 
Sequence Annotation
 METAL 242 242 Iron; catalytic (By similarity).
 METAL 244 244 Iron; catalytic (By similarity).
 METAL 389 389 Iron; catalytic (By similarity).
 MOD_RES 236 236 N6-acetyllysine.  
Keyword
 Acetylation; Alternative promoter usage; Alternative splicing; Carnitine biosynthesis; Complete proteome; Dioxygenase; Iron; Metal-binding; Mitochondrion; Oxidoreductase; Reference proteome; Transit peptide. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 421 AA 
Protein Sequence
MWYHRLSHLH SRLQDLLKGG VIYPALPQPN FKSLLPLAVH WHHTASKSLT CAWQQHEDHF 60
ELKYANTVMR FDYVWLRDHC RSASCYNSKT HQRSLDTASV DLCIKPKTIR LDETTLFFTW 120
PDGHVTKYDL NWLVKNSYEG QKQKVIQPRI LWNAEIYQQA QVPSVDCQSF LETNEGLKKF 180
LQNFLLYGIA FVENVPPTQE HTEKLAERIS LIRETIYGRM WYFTSDFSRG DTAYTKLALD 240
RHTDTTYFQE PCGIQVFHCL KHEGTGGRTL LVDGFYAAEQ VLQKAPEEFE LLSKVPLKHE 300
YIEDVGECHN HMIGIGPVLN IYPWNKELYL IRYNNYDRAV INTVPYDVVH RWYTAHRTLT 360
IELRRPENEF WVKLKPGRVL FIDNWRVLHG RECFTGYRQL CGCYLTRDDV LNTARLLGLQ 420
A 421 
Gene Ontology
 GO:0005759; C:mitochondrial matrix; TAS:Reactome.
 GO:0005739; C:mitochondrion; IDA:BHF-UCL.
 GO:0005506; F:iron ion binding; IEA:InterPro.
 GO:0031418; F:L-ascorbic acid binding; IEA:InterPro.
 GO:0016702; F:oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen; IEA:UniProtKB-KW.
 GO:0050353; F:trimethyllysine dioxygenase activity; IDA:BHF-UCL.
 GO:0045329; P:carnitine biosynthetic process; IDA:BHF-UCL.
 GO:0051354; P:negative regulation of oxidoreductase activity; IDA:BHF-UCL. 
Interpro
 IPR010376; DUF971.
 IPR003819; Taurine_dOase.
 IPR012776; Trimethyllysine_dOase. 
Pfam
 PF06155; DUF971
 PF02668; TauD 
SMART
  
PROSITE
  
PRINTS