CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-017055
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Negative elongation factor C/D 
Protein Synonyms/Alias
 NELF-C/D; TH1-like protein 
Gene Name
 NELFCD 
Gene Synonyms/Alias
 NELFD; TH1; TH1L; HSPC130 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
67PSIFNTLKRYFQAGGubiquitination[1, 2, 3, 4]
125HLKSLLIKHFDPRKAubiquitination[1, 3, 4]
272VQRFAQEKGHDASQIubiquitination[3]
380NKRVSINKDELKSTSubiquitination[3]
Reference
 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [4] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789] 
Functional Description
 Essential component of the NELF complex, a complex that negatively regulates the elongation of transcription by RNA polymerase II. The NELF complex, which acts via an association with the DSIF complex and causes transcriptional pausing, is counteracted by the P-TEFb kinase complex. 
Sequence Annotation
  
Keyword
 Alternative initiation; Alternative splicing; Complete proteome; Direct protein sequencing; Nucleus; Reference proteome; Repressor; Transcription; Transcription regulation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 590 AA 
Protein Sequence
MAGAVPGAIM DEDYYGSAAE WGDEADGGQQ EDDSGEGEDD AEVQQECLHK FSTRDYIMEP 60
SIFNTLKRYF QAGGSPENVI QLLSENYTAV AQTVNLLAEW LIQTGVEPVQ VQETVENHLK 120
SLLIKHFDPR KADSIFTEEG ETPAWLEQMI AHTTWRDLFY KLAEAHPDCL MLNFTVKLIS 180
DAGYQGEITS VSTACQQLEV FSRVLRTSLA TILDGGEENL EKNLPEFAKM VCHGEHTYLF 240
AQAMMSVLAQ EEQGGSAVRR IAQEVQRFAQ EKGHDASQIT LALGTAASYP RACQALGAML 300
SKGALNPADI TVLFKMFTSM DPPPVELIRV PAFLDLFMQS LFKPGARINQ DHKHKYIHIL 360
AYAASVVETW KKNKRVSINK DELKSTSKAV ETVHNLCCNE NKGASELVAE LSTLYQCIRF 420
PVVAMGVLKW VDWTVSEPRY FQLQTDHTPV HLALLDEIST CHQLLHPQVL QLLVKLFETE 480
HSQLDVMEQL ELKKTLLDRM VHLLSRGYVL PVVSYIRKCL EKLDTDISLI RYFVTEVLDV 540
IAPPYTSDFV QLFLPILEND SIAGTIKTEG EHDPVTEFIA HCKSNFIMVN 590 
Gene Ontology
 GO:0032021; C:NELF complex; IDA:UniProtKB.
 GO:0045892; P:negative regulation of transcription, DNA-dependent; IEA:InterPro.
 GO:0050434; P:positive regulation of viral transcription; TAS:Reactome.
 GO:0006368; P:transcription elongation from RNA polymerase II promoter; TAS:Reactome.
 GO:0022415; P:viral reproductive process; TAS:Reactome. 
Interpro
 IPR006942; TH1. 
Pfam
 PF04858; TH1 
SMART
  
PROSITE
  
PRINTS