CPLM-019944

Genbank Nucleotide ID

GrpE protein homolog 1, mitochondrial

Protein Synonyms/Alias

Mus musculus (Mouse)

Position	Peptide	Type	References
58	TDPPSADKTLLEEKA	acetylation	[1, 2, 3]
64	DKTLLEEKAKLEEQL	acetylation	[3]
66	TLLEEKAKLEEQLRE	acetylation	[1, 3, 4]
77	QLRETMEKYKRALAD	acetylation	[1, 3, 4, 5]
94	NLRQRSQKLVEEAKL	acetylation	[1, 3, 4, 6]
94	NLRQRSQKLVEEAKL	succinylation	[6]
100	QKLVEEAKLYGIQGF	acetylation	[1, 3, 4, 5, 7]
120	EVADILEKATQSVPK	acetylation	[6, 7]
120	EVADILEKATQSVPK	succinylation	[6]
127	KATQSVPKEEISNNN	acetylation	[4]
157	QIQKVFTKHGLLRLD	acetylation	[3, 5, 7]
169	RLDPIGAKFDPYEHE	acetylation	[6]
169	RLDPIGAKFDPYEHE	succinylation	[6]
215	PALVGVVKDA*****	acetylation	[1, 2, 4, 5, 6, 7]
215	PALVGVVKDA*****	succinylation	[6]

[1] Calorie restriction and SIRT3 trigger global reprogramming of the mitochondrial protein acetylome.
Hebert AS, Dittenhafer-Reed KE, Yu W, Bailey DJ, Selen ES, Boersma MD, Carson JJ, Tonelli M, Balloon AJ, Higbee AJ, Westphall MS, Pagliarini DJ, Prolla TA, Assadi-Porter F, Roy S, Denu JM, Coon JJ.
Mol Cell. 2013 Jan 10;49(1):186-99. [PMID: 23201123]
[2] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
PLoS One. 2012;7(12):e50545. [PMID: 23236377]
[3] Quantification of mitochondrial acetylation dynamics highlights prominent sites of metabolic regulation.
Still AJ, Floyd BJ, Hebert AS, Bingman CA, Carson JJ, Gunderson DR, Dolan BK, Grimsrud PA, Dittenhafer-Reed KE, Stapleton DS, Keller MP, Westphall MS, Denu JM, Attie AD, Coon JJ, Pagliarini DJ.
J Biol Chem. 2013 Jul 17;. [PMID: 23864654]
[4] Label-free quantitative proteomics of the lysine acetylome in mitochondria identifies substrates of SIRT3 in metabolic pathways.
Rardin MJ, Newman JC, Held JM, Cusack MP, Sorensen DJ, Li B, Schilling B, Mooney SD, Kahn CR, Verdin E, Gibson BW.
Proc Natl Acad Sci U S A. 2013 Apr 16;110(16):6601-6. [PMID: 23576753]
[5] The fasted/fed mouse metabolic acetylome: N6-acetylation differences suggest acetylation coordinates organ-specific fuel switching.
Yang L, Vaitheesvaran B, Hartil K, Robinson AJ, Hoopmann MR, Eng JK, Kurland IJ, Bruce JE.
J Proteome Res. 2011 Sep 2;10(9):4134-49. [PMID: 21728379]
[6] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337]
[7] Quantitative acetylome analysis reveals the roles of SIRT1 in regulating diverse substrates and cellular pathways.
Chen Y, Zhao W, Yang JS, Cheng Z, Luo H, Lu Z, Tan M, Gu W, Zhao Y.
Mol Cell Proteomics. 2012 Oct;11(10):1048-62. [PMID: 22826441]

Functional Description

Essential component of the PAM complex, a complex required for the translocation of transit peptide-containing proteins from the inner membrane into the mitochondrial matrix in an ATP-dependent manner. Seems to control the nucleotide-dependent binding of mitochondrial HSP70 to substrate proteins (By similarity).

MOD_RES 215 215 N6-acetyllysine (By similarity).

Acetylation; Chaperone; Complete proteome; Mitochondrion; Reference proteome; Transit peptide.

UniProt (SWISSPROT/TrEMBL); GenBank; EMBL

GO:0005759; C:mitochondrial matrix; IEA:UniProtKB-SubCell.
GO:0005739; C:mitochondrion; IDA:MGI.
GO:0000774; F:adenyl-nucleotide exchange factor activity; IEA:InterPro.
GO:0006457; P:protein folding; IEA:InterPro.

IPR000740; GrpE.
IPR013805; GrpE_coiled_coil.
IPR009012; GrpE_head.

PR00773; GRPEPROTEIN.