| Tag | Content |
|---|
| CPLM ID | CPLM-015675 |
UniProt Accession | |
Genbank Protein ID | |
Genbank Nucleotide ID | |
Protein Name | Calpain-8 |
Protein Synonyms/Alias | Calpain large subunit 4; New calpain 2; nCL-2; Stomach-specific M-type calpain |
Gene Name | Capn8 |
Gene Synonyms/Alias | Cls4; Ncl2 |
Created Date | July 27, 2013 |
Organism | Rattus norvegicus (Rat) |
NCBI Taxa ID | 10116 |
Lysine Modification | Position | Peptide | Type | References |
|---|
| 312 | NYIDPRRKEELDKKA | acetylation | [1] |
|
Reference | [1] Proteomic analysis of lysine acetylation sites in rat tissues reveals organ specificity and subcellular patterns. Lundby A, Lage K, Weinert BT, Bekker-Jensen DB, Secher A, Skovgaard T, Kelstrup CD, Dmytriyev A, Choudhary C, Lundby C, Olsen JV. Cell Rep. 2012 Aug 30;2(2):419-31. [ PMID: 22902405] |
Functional Description | Calcium-regulated non-lysosomal thiol-protease. Involved in membrane trafficking in the gastric surface mucus cells (pit cells) and may involve the membrane trafficking of mucus cells via interactions with coat protein. Proteolytically cleaves the beta- subunit of coatomer complex (By similarity). |
Sequence Annotation | DOMAIN 45 344 Calpain catalytic.
DOMAIN 532 566 EF-hand 1.
DOMAIN 575 608 EF-hand 2.
DOMAIN 605 640 EF-hand 3.
DOMAIN 670 703 EF-hand 4.
REGION 355 512 Domain III.
REGION 513 531 Linker (By similarity).
REGION 532 703 Domain IV (By similarity).
ACT_SITE 105 105 By similarity.
ACT_SITE 262 262 By similarity.
ACT_SITE 286 286 By similarity. |
Keyword | Alternative splicing; Autocatalytic cleavage; Calcium; Complete proteome; Cytoplasm; Golgi apparatus; Hydrolase; Metal-binding; Protease; Reference proteome; Repeat; Thiol protease. |
Sequence Source | UniProt (SWISSPROT/TrEMBL); GenBank; EMBL |
Protein Length | 703 AA |
Protein Sequence | MAALAAGVSK QRAVAEGLGS NQNAVKYLGQ DFETLRKQCL NSGVLFKDPE FPACPSALGY 60
KDLGPGSPDT QGIVWKRPTE LCPNPQFIVG GATRTDIRQG GLGDCWLLAA IASLTLNEKL 120
LYRVLPRDQS FQKDYAGIFH FQFWQYGEWV EVVIDDRLPT KNGQLLFLHS EEGNEFWSAL 180
LEKAYAKLNG SYEALVGGST IEGFEDFTGG ISEFYDLKKP PENLYYIIQK ALRKGSLLGC 240
SIDVSTAAEA EATTRQKLVK GHAYSVTGVE EVNFHGRPEK LIRLRNPWGE VEWSGAWSDN 300
APEWNYIDPR RKEELDKKAE DGEFWMSFSD FLKQYSRLEI CNLSPDSLSS EEIHKWNLVL 360
FNGRWTRGST AGGCLNYPGT YWTNPQFKIH LDEVDEDQEE GTSEPCCTVL LGLMQKNRRR 420
QKRIGQGMLS IGYAVYQIPK ELESHTDAHL GRDFFLGRQP STCSSTYMNL REVSSRVRLP 480
PGQYLVVPST FEPFKDGDFC LRVFSEKKAK ALEIGDTVSG HPHEPHPRDM DEEDEHVRSL 540
FEEFVGKDSE ISANQLKRVL NEVLSKRTDM KFDGFNINTC REMISLLDSD GTGSLGPMEF 600
KTLWLKIRTY LEIFQEMDHN HVGTIEAHEM RTALKKAGFT LNNQVQQTIA MRYACSKLGV 660
DFNGFVACMI RLETLFKLFR LLDKDQNGIV QLSLAEWLCC VLV 703 |
Gene Ontology | GO:0005794; C:Golgi apparatus; IEA:UniProtKB-SubCell. GO:0005509; F:calcium ion binding; IEA:InterPro. GO:0004198; F:calcium-dependent cysteine-type endopeptidase activity; IEA:InterPro. GO:0006508; P:proteolysis; IEA:UniProtKB-KW. |
Interpro | |
Pfam | |
SMART | |
PROSITE | |
PRINTS | |