CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-023853
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Hypoxia up-regulated protein 1 
Protein Synonyms/Alias
 150 kDa oxygen-regulated protein; ORP-150; 170 kDa glucose-regulated protein; GRP-170 
Gene Name
 HYOU1 
Gene Synonyms/Alias
 GRP170; ORP150 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
51SMKVAIVKPGVPMEIubiquitination[1]
62PMEIVLNKESRRKTPubiquitination[1]
91SAASMAIKNPKATLRubiquitination[1, 2, 3, 4]
94SMAIKNPKATLRYFQubiquitination[1]
106YFQHLLGKQADNPHVubiquitination[1]
170DFAEQPIKDAVITVPubiquitination[3]
308ENPRAMAKLLREANRubiquitination[1]
398RVQEVLLKAVGKEELubiquitination[2, 4]
402VLLKAVGKEELGKNIubiquitination[1]
463EPGIHSLKHNKRVLFubiquitination[1]
520SQNLTTVKLKGVGDSubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [3] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [4] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789] 
Functional Description
 Has a pivotal role in cytoprotective cellular mechanisms triggered by oxygen deprivation. May play a role as a molecular chaperone and participate in protein folding. 
Sequence Annotation
 MOTIF 996 999 Prevents secretion from ER (Potential).
 CARBOHYD 155 155 N-linked (GlcNAc...).
 CARBOHYD 222 222 N-linked (GlcNAc...) (Potential).
 CARBOHYD 515 515 N-linked (GlcNAc...).
 CARBOHYD 596 596 N-linked (GlcNAc...).
 CARBOHYD 830 830 N-linked (GlcNAc...).
 CARBOHYD 862 862 N-linked (GlcNAc...).
 CARBOHYD 869 869 N-linked (GlcNAc...).
 CARBOHYD 922 922 N-linked (GlcNAc...) (Potential).
 CARBOHYD 931 931 N-linked (GlcNAc...).  
Keyword
 ATP-binding; Chaperone; Complete proteome; Direct protein sequencing; Endoplasmic reticulum; Glycoprotein; Nucleotide-binding; Reference proteome; Signal; Stress response. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 999 AA 
Protein Sequence
MADKVRRQRP RRRVCWALVA VLLADLLALS DTLAVMSVDL GSESMKVAIV KPGVPMEIVL 60
NKESRRKTPV IVTLKENERF FGDSAASMAI KNPKATLRYF QHLLGKQADN PHVALYQARF 120
PEHELTFDPQ RQTVHFQISS QLQFSPEEVL GMVLNYSRSL AEDFAEQPIK DAVITVPVFF 180
NQAERRAVLQ AARMAGLKVL QLINDNTATA LSYGVFRRKD INTTAQNIMF YDMGSGSTVC 240
TIVTYQMVKT KEAGMQPQLQ IRGVGFDRTL GGLEMELRLR ERLAGLFNEQ RKGQRAKDVR 300
ENPRAMAKLL REANRLKTVL SANADHMAQI EGLMDDVDFK AKVTRVEFEE LCADLFERVP 360
GPVQQALQSA EMSLDEIEQV ILVGGATRVP RVQEVLLKAV GKEELGKNIN ADEAAAMGAV 420
YQAAALSKAF KVKPFVVRDA VVYPILVEFT REVEEEPGIH SLKHNKRVLF SRMGPYPQRK 480
VITFNRYSHD FNFHINYGDL GFLGPEDLRV FGSQNLTTVK LKGVGDSFKK YPDYESKGIK 540
AHFNLDESGV LSLDRVESVF ETLVEDSAEE ESTLTKLGNT ISSLFGGGTT PDAKENGTDT 600
VQEEEESPAE GSKDEPGEQV ELKEEAEAPV EDGSQPPPPE PKGDATPEGE KATEKENGDK 660
SEAQKPSEKA EAGPEGVAPA PEGEKKQKPA RKRRMVEEIG VELVVLDLPD LPEDKLAQSV 720
QKLQDLTLRD LEKQEREKAA NSLEAFIFET QDKLYQPEYQ EVSTEEQREE ISGKLSAAST 780
WLEDEGVGAT TVMLKEKLAE LRKLCQGLFF RVEERKKWPE RLSALDNLLN HSSMFLKGAR 840
LIPEMDQIFT EVEMTTLEKV INETWAWKNA TLAEQAKLPA TEKPVLLSKD IEAKMMALDR 900
EVQYLLNKAK FTKPRPRPKD KNGTRAEPPL NASASDQGEK VIPPAGQTED AEPISEPEKV 960
ETGSEPGDTE PLELGGPGAE PEQKEQSTGQ KRPLKNDEL 999 
Gene Ontology
 GO:0005788; C:endoplasmic reticulum lumen; TAS:Reactome.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0006987; P:activation of signaling protein activity involved in unfolded protein response; TAS:Reactome.
 GO:0044267; P:cellular protein metabolic process; TAS:Reactome. 
Interpro
 IPR018181; Heat_shock_70_CS.
 IPR013126; Hsp_70_fam. 
Pfam
 PF00012; HSP70 
SMART
  
PROSITE
 PS00297; HSP70_1
 PS00329; HSP70_2
 PS01036; HSP70_3 
PRINTS
 PR00301; HEATSHOCK70.