CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-016642
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Serine/threonine-protein phosphatase PGAM5, mitochondrial 
Protein Synonyms/Alias
 Phosphoglycerate mutase family member 5 
Gene Name
 Pgam5 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
92TSRLDHYKAKATRHIacetylation[1]
94RLDHYKAKATRHIFLacetylation[1]
140RLASLGLKFNKIVHSubiquitination[2]
190DPPVSHWKPEAVQYYacetylation[1, 3]
Reference
 [1] Quantification of mitochondrial acetylation dynamics highlights prominent sites of metabolic regulation.
 Still AJ, Floyd BJ, Hebert AS, Bingman CA, Carson JJ, Gunderson DR, Dolan BK, Grimsrud PA, Dittenhafer-Reed KE, Stapleton DS, Keller MP, Westphall MS, Denu JM, Attie AD, Coon JJ, Pagliarini DJ.
 J Biol Chem. 2013 Jul 17;. [PMID: 23864654]
 [2] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [3] Calorie restriction and SIRT3 trigger global reprogramming of the mitochondrial protein acetylome.
 Hebert AS, Dittenhafer-Reed KE, Yu W, Bailey DJ, Selen ES, Boersma MD, Carson JJ, Tonelli M, Balloon AJ, Higbee AJ, Westphall MS, Pagliarini DJ, Prolla TA, Assadi-Porter F, Roy S, Denu JM, Coon JJ.
 Mol Cell. 2013 Jan 10;49(1):186-99. [PMID: 23201123
Functional Description
 Displays phosphatase activity for serine/threonine residues, and, dephosphorylates and activates MAP3K5 kinase. Has apparently no phosphoglycerate mutase activity. May be regulator of mitochondrial dynamics. Substrate for a KEAP1-dependent ubiquitin ligase complex. Contributes to the repression of NFE2L2- dependent gene expression (By similarity). Acts as a central mediator for programmed necrosis induced by TNF, by reactive oxygen species and by calcium ionophore. 
Sequence Annotation
 REGION 76 81 Interaction with KEAP1 (By similarity).
 MOD_RES 79 79 Phosphoserine (By similarity).
 MOD_RES 115 115 N6-acetyllysine (By similarity).
 MOD_RES 143 143 N6-acetyllysine (By similarity).
 MOD_RES 190 190 N6-acetyllysine (By similarity).  
Keyword
 Acetylation; Alternative splicing; Complete proteome; Hydrolase; Membrane; Mitochondrion; Mitochondrion outer membrane; Necrosis; Phosphoprotein; Reference proteome; Transmembrane; Transmembrane helix. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 288 AA 
Protein Sequence
MAFRQALQLA ACGLAGGSAA VLFSAVAVGK PRGGGDADTR ATEPPAWTGA RAGRGVWDTN 60
WDRREPLSLI NLKKRNVESG EDELTSRLDH YKAKATRHIF LIRHSQYHVD GSLEKDRTLT 120
PLGREQAELT GLRLASLGLK FNKIVHSSMT RAVETTDIIS KHLPGVSRVS TDLLREGAPI 180
EPDPPVSHWK PEAVQYYEDG ARIEAAFRNY IHRADARQEE DSYEIFICHA NVIRYIVCRA 240
LQFPPEGWLR LSLNNGSITH LVIRPNGRVA LRTLGDTGFM PPDKITRS 288 
Gene Ontology
 GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
 GO:0005741; C:mitochondrial outer membrane; IEA:UniProtKB-SubCell.
 GO:0004721; F:phosphoprotein phosphatase activity; IEA:EC. 
Interpro
 IPR013078; His_Pase_superF_clade-1. 
Pfam
 PF00300; His_Phos_1 
SMART
 SM00855; PGAM 
PROSITE
 PS00175; PG_MUTASE 
PRINTS