CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-014305
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Serine/threonine-protein kinase PRP4 homolog 
Protein Synonyms/Alias
 PRP4 pre-mRNA-processing factor 4 homolog; Pre-mRNA protein kinase 
Gene Name
 Prpf4b 
Gene Synonyms/Alias
 Cbp143; Prp4h; Prp4k; Prp4m; Prpk 
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
100LSPAKRTKLDDLALLacetylation[1]
112ALLEDLEKQRALIKAubiquitination[2]
171RSSSTRGKLEITDNKacetylation[1]
656LRAAGIGKDFKENPNacetylation[1, 3]
737LKELEFLKKLNDADPubiquitination[2]
Reference
 [1] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
 Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
 Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337]
 [2] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [3] Quantitative acetylome analysis reveals the roles of SIRT1 in regulating diverse substrates and cellular pathways.
 Chen Y, Zhao W, Yang JS, Cheng Z, Luo H, Lu Z, Tan M, Gu W, Zhao Y.
 Mol Cell Proteomics. 2012 Oct;11(10):1048-62. [PMID: 22826441] 
Functional Description
 Has a role in pre-mRNA splicing. Phosphorylates SF2/ASF. 
Sequence Annotation
 DOMAIN 687 1006 Protein kinase.
 NP_BIND 693 701 ATP (By similarity).
 ACT_SITE 815 815 Proton acceptor (By similarity).
 BINDING 717 717 ATP (By similarity).
 MOD_RES 2 2 N-acetylalanine (By similarity).
 MOD_RES 21 21 Phosphoserine (By similarity).
 MOD_RES 24 24 Phosphoserine (By similarity).
 MOD_RES 33 33 Phosphoserine (By similarity).
 MOD_RES 88 88 Phosphoserine.
 MOD_RES 94 94 Phosphoserine.
 MOD_RES 141 141 Phosphotyrosine (By similarity).
 MOD_RES 143 143 Phosphoserine (By similarity).
 MOD_RES 145 145 Phosphoserine (By similarity).
 MOD_RES 240 240 Phosphoserine (By similarity).
 MOD_RES 242 242 Phosphoserine (By similarity).
 MOD_RES 258 258 Phosphoserine (By similarity).
 MOD_RES 278 278 Phosphoserine.
 MOD_RES 292 292 Phosphoserine.
 MOD_RES 294 294 Phosphoserine.
 MOD_RES 328 328 Phosphoserine (By similarity).
 MOD_RES 354 354 Phosphoserine (By similarity).
 MOD_RES 356 356 Phosphoserine (By similarity).
 MOD_RES 366 366 Phosphoserine.
 MOD_RES 368 368 Phosphoserine.
 MOD_RES 385 385 Phosphothreonine (By similarity).
 MOD_RES 387 387 Phosphoserine (By similarity).
 MOD_RES 427 427 Phosphoserine.
 MOD_RES 431 431 Phosphoserine.
 MOD_RES 437 437 Phosphoserine.
 MOD_RES 518 518 Phosphoserine (By similarity).
 MOD_RES 519 519 Phosphoserine (By similarity).
 MOD_RES 520 520 Phosphoserine (By similarity).
 MOD_RES 565 565 Phosphoserine (By similarity).
 MOD_RES 569 569 Phosphoserine (By similarity).
 MOD_RES 576 576 Phosphoserine.
 MOD_RES 578 578 Phosphoserine.
 MOD_RES 580 580 Phosphoserine.
 MOD_RES 717 717 N6-acetyllysine (By similarity).
 MOD_RES 849 849 Phosphotyrosine.
 MOD_RES 852 852 Phosphoserine.  
Keyword
 Acetylation; ATP-binding; Complete proteome; Kinase; mRNA processing; mRNA splicing; Nucleotide-binding; Nucleus; Phosphoprotein; Reference proteome; Serine/threonine-protein kinase; Spliceosome; Transferase. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1007 AA 
Protein Sequence
MAATEPPSLR EQAEMDDADN SEKSVNEENG EVSEDQSQNK HSRHKKKKHK HRSKHKKHKH 60
SSEEDRDKKH KHKHKHKKHK RKEVIEASDK EGLSPAKRTK LDDLALLEDL EKQRALIKAE 120
LDNELMEGKV QSGMGLILQG YESGSEEEGE IHEKARNGNR SSTRSSSTRG KLEITDNKNS 180
AKKRSKSRSK ERTRHRSDKR KSKGAGEMLR EKANRSKSKE RRKSKSPSKR SKSQDQARKS 240
KSPPLRRRSQ EKVGKARSPA EEKMKSEEKG KIKDRKKSPI VNERSRDRSK KSKSPVDLRD 300
KSKDRRSRSK ERKSKRSEID KEKKPIKSPS KDASSGKENR SPSRRPGRSP KRRSLSPKLR 360
DKSRRSRSPL LNDRRSKQSK SPSRTLSPGR RAKSRSLERK RREPERRRLS SPRTRPRDDI 420
LGRCERSKDA SPINRWSPTR RRSRSPIRRR SRSPLRRSRS PRRRSRSPRR RDRSRRSRSR 480
LRRRSRSRGG HRRRSRSKVK EDKFKGSLSE GMKVEQESSS DDNLEDFDVE EEDEEALIEQ 540
RRIQRQAIVQ KYKYLAEDSN ISVPSEPSSP QSSTRSRSPS PDDILERVAA DVKEYERENV 600
DTFEASVKAK HNLMTVEQNN GSSQKKILAP DMFTESDDMF AAYFDSARLR AAGIGKDFKE 660
NPNLRDNWTD AEGYYRVNIG EVLDKRYNVY GYTGQGVFSN VVRARDNARA NQEVAVKIIR 720
NNELMQKTGL KELEFLKKLN DADPDDKFHC LRLFRHFYHK QHLCLVFEPL SMNLREVLKK 780
YGKDVGLHIK AVRSYSQQLF LALKLLKRCN ILHADIKPDN ILVNESKTIL KLCDFGSASH 840
VADNDITPYL VSRFYRAPEI IIGKSYDYGI DMWSVGCTLY ELYTGKILFP GKTNNHMLKL 900
AMDLKGKMPN KMIRKGVFKD QHFDQNLNFM YIEVDKVTER EKVTVMSTIN PTKDLLADLI 960
GCQRLPEDQR KKVHQLKDLL DQILMLDPAK RISINQALQH AFIQEKI 1007 
Gene Ontology
 GO:0071013; C:catalytic step 2 spliceosome; IEA:Compara.
 GO:0005694; C:chromosome; IDA:MGI.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0004672; F:protein kinase activity; ISS:MGI.
 GO:0004674; F:protein serine/threonine kinase activity; IEA:UniProtKB-KW.
 GO:0006397; P:mRNA processing; IEA:UniProtKB-KW.
 GO:0008380; P:RNA splicing; IEA:UniProtKB-KW. 
Interpro
 IPR011009; Kinase-like_dom.
 IPR000719; Prot_kinase_cat_dom.
 IPR002290; Ser/Thr_dual-sp_kinase_dom.
 IPR008271; Ser/Thr_kinase_AS. 
Pfam
 PF00069; Pkinase 
SMART
 SM00220; S_TKc 
PROSITE
 PS00107; PROTEIN_KINASE_ATP
 PS50011; PROTEIN_KINASE_DOM
 PS00108; PROTEIN_KINASE_ST 
PRINTS