CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-033173
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
  
Protein Name
 Mitogen-activated protein kinase kinase kinase kinase 4 
Protein Synonyms/Alias
  
Gene Name
 MAP4K4 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
46VYKGRHVKTGQLAAIubiquitination[1]
401PPQQERSKPSFHAPEubiquitination[1]
634EPAMTPSKEGTLIVRubiquitination[1]
654SSTLQKHKSSSSFTPubiquitination[1, 2]
701IRQDPTRKGSVVNVNubiquitination[1]
760LDRSGQGKVYPLINRubiquitination[1]
813HNDPEVEKKQGWTTVubiquitination[3]
814NDPEVEKKQGWTTVGubiquitination[1]
955NTYGRITKDVVLQWGubiquitination[1, 4]
982QTMGWGEKAIEIRSVubiquitination[1, 4]
1001LDGVFMHKRAQRLKFubiquitination[1, 5]
1007HKRAQRLKFLCERNDubiquitination[1, 4]
1015FLCERNDKVFFASVRubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [3] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [4] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [5] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473
Functional Description
  
Sequence Annotation
  
Keyword
 ATP-binding; Complete proteome; Nucleotide-binding; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1042 AA 
Protein Sequence
MANDSPAKSL VDIDLSSLRD PAGIFELVEV VGNGTYGQVY KGRHVKTGQL AAIKVMDVTE 60
DEEEEIKLEI NMLKKYSHHR NIATYYGAFI KKSPPGHDDQ LWLVMEFCGA GSITDLVKNT 120
KGNTLKEDWI AYISREILRG LAHLHIHHVI HRDIKGQNVL LTENAEVKLD ETEYEYSGSE 180
EEEEEVPEQE GEPSSIVNVP GESTLRRDFL RLQQENKERS EALRRQQLLQ EQQLREQEEY 240
KRQLLAERQK RIEQQKEQRR RLEEQQRRER EARRQQEREQ RRREQEEKRR LEELERRRKE 300
EEERRRAEEE KRRVEREQEY IRRQLEEEQR HLEVLQQQLL QEQAMLLECR WREMEEHRQA 360
ERLQRQLQQE QAYLLSLQHD HRRPHPQHSQ QPPPPQQERS KPSFHAPEPK AHYEPADRAR 420
EVPVRTTSRS PVLSRRDSPL QGSGQQNSQA GQRNSTSSIE PRLLWERVEK LVPRPGSGSS 480
SGSSNSGSQP GSHPGSQSGS GERFRVRSSS KSEGSPSQRL ENAVKKPEDK KEVFRPLKPA 540
VRIDLTALAK ELRAVEDVRP PHKVTDYSSS SEESGTTDEE DDDVEQEGAD ESTSGPEDTR 600
AASSLNLSNG ETESVKTMIV HDDVESEPAM TPSKEGTLIV RQTQSASSTL QKHKSSSSFT 660
PFIDPRLLQI SPSSGTTVTS VVGFSCDGMR PEAIRQDPTR KGSVVNVNPT NTRPQSDTPE 720
IRKYKKRFNS EILCAALWGV NLLVGTESGL MLLDRSGQGK VYPLINRRRF QQMDVLEGLN 780
VLVTISGKKD KLRVYYLSWL RNKILHNDPE VEKKQGWTTV GDLEGCVHYK VVKYERIKFL 840
VIALKSSVEV YAWAPKPYHK FMAFKSFGEL VHKPLLVDLT VEEGQRLKVI YGSCAGFHAV 900
DVDSGSVYDI YLPTHIQCSI KPHAIIILPN TDGMELLVCY EDEGVYVNTY GRITKDVVLQ 960
WGEMPTSVAY IRSNQTMGWG EKAIEIRSVE TGHLDGVFMH KRAQRLKFLC ERNDKVFFAS 1020
VRSGGSSQVY FMTLGRTSLL SW 1042 
Gene Ontology
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0004674; F:protein serine/threonine kinase activity; IEA:InterPro.
 GO:0005083; F:small GTPase regulator activity; IEA:InterPro. 
Interpro
 IPR001180; Citron.
 IPR011009; Kinase-like_dom.
 IPR000719; Prot_kinase_cat_dom.
 IPR017441; Protein_kinase_ATP_BS.
 IPR008271; Ser/Thr_kinase_AS. 
Pfam
 PF00780; CNH
 PF00069; Pkinase 
SMART
 SM00036; CNH 
PROSITE
 PS50219; CNH
 PS00107; PROTEIN_KINASE_ATP
 PS50011; PROTEIN_KINASE_DOM
 PS00108; PROTEIN_KINASE_ST 
PRINTS