CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-011434
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Glycogen debranching enzyme 
Protein Synonyms/Alias
 Glycogen debrancher; 4-alpha-glucanotransferase; Oligo-1,4-1,4-glucantransferase; Amylo-alpha-1,6-glucosidase; Amylo-1,6-glucosidase; Dextrin 6-alpha-D-glucosidase 
Gene Name
 GDB1 
Gene Synonyms/Alias
 YPR184W 
Created Date
 July 27, 2013 
Organism
 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 
NCBI Taxa ID
 559292 
Lysine Modification
Position
Peptide
Type
References
1256DKMGESEKAGSVGIPubiquitination[1]
Reference
 [1] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, VillĂ©n J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301
Functional Description
 Multifunctional enzyme acting as 1,4-alpha-D-glucan:1,4- alpha-D-glucan 4-alpha-D-glycosyltransferase and amylo-1,6- glucosidase in glycogen degradation. 
Sequence Annotation
 REGION 1 ? 4-alpha-glucanotransferase.
 REGION ? 1536 Amylo-1,6-glucosidase.
 ACT_SITE 535 535 By similarity.
 ACT_SITE 538 538 By similarity.
 ACT_SITE 670 670 By similarity.  
Keyword
 Complete proteome; Cytoplasm; Direct protein sequencing; Glycogen biosynthesis; Glycosidase; Glycosyltransferase; Hydrolase; Mitochondrion; Multifunctional enzyme; Reference proteome; Transferase. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1536 AA 
Protein Sequence
MNRSLLLRLS DTGEPITSCS YGKGVLTLPP IPLPKDAPKD QPLYTVKLLV SAGSPVARDG 60
LVWTNCPPDH NTPFKRDKFY KKIIHSSFHE DDCIDLNVYA PGSYCFYLSF RNDNEKLETT 120
RKYYFVALPM LYINDQFLPL NSIALQSVVS KWLGSDWEPI LSKIAAKNYN MVHFTPLQER 180
GESNSPYSIY DQLQFDQEHF KSPEDVKNLV EHIHRDLNML SLTDIVFNHT ANNSPWLVEH 240
PEAGYNHITA PHLISAIELD QELLNFSRNL KSWGYPTELK NIEDLFKIMD GIKVHVLGSL 300
KLWEYYAVNV QTALRDIKAH WNDESNESYS FPENIKDISS DFVKLASFVK DNVTEPNFGT 360
LGERNSNRIN VPKFIQLLKL INDGGSDDSE SSLATAQNIL NEVNLPLYRE YDDDVSEILE 420
QLFNRIKYLR LDDGGPKQGP VTVDVPLTEP YFTRFKGKDG TDYALANNGW IWNGNPLVDF 480
ASQNSRAYLR REVIVWGDCV KLRYGKSPED SPYLWERMSK YIEMNAKIFD GFRIDNCHST 540
PIHVGEYFLD LARKYNPNLY VVAELFSGSE TLDCLFVERL GISSLIREAM QAWSEEELSR 600
LVHKHGGRPI GSYKFVPMDD FSYPADINLN EEHCFNDSND NSIRCVSEIM IPKILTATPP 660
HALFMDCTHD NETPFEKRTV EDTLPNAALV ALCSSAIGSV YGYDEIFPHL LNLVTEKRHY 720
DISTPTGSPS IGITKVKATL NSIRTSIGEK AYDIEDSEMH VHHQGQYITF HRMDVKSGKG 780
WYLIARMKFS DNDDPNETLP PVVLNQSTCS LRFSYALERV GDEIPNDDKF IKGIPTKLKE 840
LEGFDISYDD SKKISTIKLP NEFPQGSIAI FETQQNGVDE SLDHFIRSGA LKATSSLTLE 900
SINSVLYRSE PEEYDVSAGE GGAYIIPNFG KPVYCGLQGW VSVLRKIVFY NDLAHPLSAN 960
LRNGHWALDY TISRLNYYSD EAGINEVQNW LRSRFDRVKK LPSYLVPSYF ALIIGILYGC 1020
CRLKAIQLMS RNIGKSTLFV QSLSMTSIQM VSRMKSTSIL PGENVPSMAA GLPHFSVNYM 1080
RCWGRDVFIS LRGMLLTTGR FDEAKAHILA FAKTLKHGLI PNLLDAGRNP RYNARDAAWF 1140
FLQAVQDYVY IVPDGEKILQ EQVTRRFPLD DTYIPVDDPR AFSYSSTLEE IIYEILSRHA 1200
KGIKFREANA GPNLDRVMTD KGFNVEIHVD WSTGLIHGGS QYNCGTWMDK MGESEKAGSV 1260
GIPGTPRDGA AIEINGLLKS ALRFVIELKN KGLFKFSDVE TQDGGRIDFT EWNQLLQDNF 1320
EKRYYVPEDP SQDADYDVSA KLGVNRRGIY RDLYKSGKPY EDYQLRPNFA IAMTVAPELF 1380
VPEHAIKAIT IADEVLRGPV GMRTLDPSDY NYRPYYNNGE DSDDFATSKG RNYHQGPEWV 1440
WLYGYFLRAF HHFHFKTSPR CQNAAKEKPS SYLYQQLYYR LKGHRKWIFE SVWAGLTELT 1500
NKDGEVCNDS SPTQAWSSAC LLDLFYDLWD AYEDDS 1536 
Gene Ontology
 GO:0005739; C:mitochondrion; IDA:SGD.
 GO:0005634; C:nucleus; IBA:RefGenome.
 GO:0004134; F:4-alpha-glucanotransferase activity; IDA:SGD.
 GO:0004135; F:amylo-alpha-1,6-glucosidase activity; IDA:SGD.
 GO:0005978; P:glycogen biosynthetic process; IEA:UniProtKB-KW.
 GO:0005980; P:glycogen catabolic process; IMP:SGD. 
Interpro
 IPR008928; 6-hairpin_glycosidase-like.
 IPR010401; GDE_C.
 IPR006047; Glyco_hydro_13_cat_dom.
 IPR013781; Glyco_hydro_catalytic_dom.
 IPR006421; Glycogen_debranch_met.
 IPR017853; Glycoside_hydrolase_SF. 
Pfam
 PF00128; Alpha-amylase
 PF06202; GDE_C 
SMART
  
PROSITE
  
PRINTS