CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-005030
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Protein-glutamine gamma-glutamyltransferase 2 
Protein Synonyms/Alias
 Tissue transglutaminase; Transglutaminase C; TG(C); TGC; TGase C; Transglutaminase-2; TGase-2 
Gene Name
 Tgm2 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
425RSLVVGQKISTKSVGubiquitination[1]
458EEREVFTKANHLNKLubiquitination[1]
468HLNKLAEKEETGVAMacetylation[2]
589YLENPEIKIRVLGEPubiquitination[1]
648VPAGDLVKARVDLFPacetylation[3]
648VPAGDLVKARVDLFPubiquitination[1]
Reference
 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [2] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
 Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
 Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337]
 [3] Quantification of mitochondrial acetylation dynamics highlights prominent sites of metabolic regulation.
 Still AJ, Floyd BJ, Hebert AS, Bingman CA, Carson JJ, Gunderson DR, Dolan BK, Grimsrud PA, Dittenhafer-Reed KE, Stapleton DS, Keller MP, Westphall MS, Denu JM, Attie AD, Coon JJ, Pagliarini DJ.
 J Biol Chem. 2013 Jul 17;. [PMID: 23864654] 
Functional Description
 Catalyzes the cross-linking of proteins and the conjugation of polyamines to proteins. 
Sequence Annotation
 ACT_SITE 277 277 By similarity.
 ACT_SITE 335 335 By similarity.
 ACT_SITE 358 358 By similarity.
 METAL 398 398 Calcium (By similarity).
 METAL 400 400 Calcium (By similarity).
 METAL 447 447 Calcium (By similarity).
 METAL 452 452 Calcium (By similarity).
 MOD_RES 2 2 N-acetylalanine.  
Keyword
 Acetylation; Acyltransferase; Calcium; Complete proteome; Direct protein sequencing; Metal-binding; Reference proteome; Transferase. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 686 AA 
Protein Sequence
MAEELLLERC DLEIQANGRD HHTADLCQEK LVLRRGQRFR LTLYFEGRGY EASVDSLTFG 60
AVTGPDPSEE AGTKARFSLS DNVEEGSWSA SVLDQQDNVL SLQLCTPANA PIGLYRLSLE 120
ASTGYQGSSF VLGHFILLYN AWCPADDVYL DSEEERREYV LTQQGFIYQG SVKFIKSVPW 180
NFGQFEDGIL DTCLMLLDMN PKFLKNRSRD CSRRSSPIYV GRVVSAMVNC NDDQGVLLGR 240
WDNNYGDGIS PMAWIGSVDI LRRWKEHGCQ QVKYGQCWVF AAVACTVLRC LGIPTRVVTN 300
YNSAHDQNSN LLIEYFRNEF GELESNKSEM IWNFHCWVES WMTRPDLQPG YEGWQAIDPT 360
PQEKSEGTYC CGPVSVRAIK EGDLSTKYDA PFVFAEVNAD VVDWIRQEDG SVLKSINRSL 420
VVGQKISTKS VGRDDREDIT HTYKYPEGSP EEREVFTKAN HLNKLAEKEE TGVAMRIRVG 480
DSMSMGNDFD VFAHIGNDTS ETRECRLLLC ARTVSYNGVL GPECGTEDIN LTLDPYSENS 540
IPLRILYEKY SGCLTESNLI KVRGLLIEPA ANSYLLAERD LYLENPEIKI RVLGEPKQNR 600
KLVAEVSLKN PLSDPLYDCI FTVEGAGLTK EQKSVEVSDP VPAGDLVKAR VDLFPTDIGL 660
HKLVVNFQCD KLKSVKGYRN VIIGPA 686 
Gene Ontology
 GO:0005829; C:cytosol; TAS:UniProtKB.
 GO:0016020; C:membrane; TAS:UniProtKB.
 GO:0005739; C:mitochondrion; IEA:Compara.
 GO:0005886; C:plasma membrane; IEA:Compara.
 GO:0005578; C:proteinaceous extracellular matrix; TAS:UniProtKB.
 GO:0005509; F:calcium ion binding; NAS:UniProtKB.
 GO:0005525; F:GTP binding; NAS:UniProtKB.
 GO:0003810; F:protein-glutamine gamma-glutamyltransferase activity; IMP:UniProtKB.
 GO:0008483; F:transaminase activity; ISO:MGI.
 GO:0043277; P:apoptotic cell clearance; IEA:Compara.
 GO:0001974; P:blood vessel remodeling; IEA:Compara.
 GO:0060445; P:branching involved in salivary gland morphogenesis; IMP:MGI.
 GO:0051482; P:elevation of cytosolic calcium ion concentration involved in phospholipase C-activating G-protein coupled signaling pathway; IEA:Compara.
 GO:0006917; P:induction of apoptosis; IEA:Compara.
 GO:0018153; P:isopeptide cross-linking via N6-(L-isoglutamyl)-L-lysine; IEA:Compara.
 GO:0018149; P:peptide cross-linking; TAS:UniProtKB.
 GO:0007200; P:phospholipase C-activating G-protein coupled receptor signaling pathway; ISA:MGI.
 GO:0045785; P:positive regulation of cell adhesion; IMP:UniProtKB.
 GO:0043123; P:positive regulation of I-kappaB kinase/NF-kappaB cascade; IEA:Compara.
 GO:0050729; P:positive regulation of inflammatory response; IEA:Compara.
 GO:0048661; P:positive regulation of smooth muscle cell proliferation; IEA:Compara.
 GO:0051260; P:protein homooligomerization; IEA:Compara.
 GO:0060662; P:salivary gland cavitation; IMP:MGI. 
Interpro
 IPR023608; Gln_gamma-glutamylTfrase_euk.
 IPR013783; Ig-like_fold.
 IPR014756; Ig_E-set.
 IPR002931; Transglutaminase-like.
 IPR008958; Transglutaminase_C.
 IPR013808; Transglutaminase_CS.
 IPR001102; Transglutaminase_N. 
Pfam
 PF00927; Transglut_C
 PF01841; Transglut_core
 PF00868; Transglut_N 
SMART
 SM00460; TGc 
PROSITE
 PS00547; TRANSGLUTAMINASES 
PRINTS