CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-041400
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
  
Protein Name
 DDB1- and CUL4-associated factor 8 
Protein Synonyms/Alias
  
Gene Name
 DCAF8 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
390LDFESGHKSNVFQAKubiquitination[1, 2, 3, 4, 5]
397KSNVFQAKFLPNSGDubiquitination[3, 4]
428LSATQCCKNTKRVAQubiquitination[4]
431TQCCKNTKRVAQHKGubiquitination[4, 5]
475RQDRPASKLVVTKEKubiquitination[4]
517VRIYDQRKIDENENNubiquitination[4]
528NENNGVLKKFCPHHLubiquitination[2, 4]
529ENNGVLKKFCPHHLVubiquitination[5]
592HRNNATVKGVNFYGPubiquitination[1, 2, 3, 4]
678GLKDVIKKNKRERDEubiquitination[4]
Reference
 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [4] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [5] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Reference proteome; Repeat; WD repeat. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 751 AA 
Protein Sequence
MSEEELTKAM EGLGMDEGDG EGNILPIMQS IMQNLLSKDV LYPSLKEITE KYPEWLQSHR 60
ESLPPEQFEK YQEQHSVMCK ICEQFEAETP TDSETTQKAR FEMVLDLMQQ LQDLGHPPKE 120
LAGEMLTWLF FCGSQWVYGP WLTYVGWLQQ TSFKMSSKGS STDGRTDLAN GSLSSSPEEM 180
SGAEEGRETS SGIEVEASDL SLSLTGDDGG PNRTSTESRG TDTESSGEDK DSDSMEDTGH 240
YSINDENRVH DRSEEEEEEE EEEEEEQPRR RVQRKRANRD QDSSDDERAL EDWVSSETSA 300
LPRPRWQALP ALRERELGSS ARFVYEACGA RVFVQRFRLQ HGLEGHTGCV NTLHFNQRGT 360
WLASGSDDLK VVVWDWVRRQ PVLDFESGHK SNVFQAKFLP NSGDSTLAMC ARDGQVRVAE 420
LSATQCCKNT KRVAQHKGAS HKLALEPDSP CTFLSAGEDA VVFTIDLRQD RPASKLVVTK 480
EKEKKVGLYT IYVNPANTHQ FAVGGRDQFV RIYDQRKIDE NENNGVLKKF CPHHLVNSES 540
KANITCLVYS HDGTELLASY NDEDIYLFNS SHSDGAQYVK RYKGHRNNAT VKGVNFYGPK 600
SEFVVSGSDC GHIFLWEKSS CQIIQFMEGD KGGVVNCLEP HPHLPVLATS GLDHDVKIWA 660
PTAEASTELT GLKDVIKKNK RERDEDSLHQ TDLFDSHMLW FLMHHLRQRR HHRRWREPGV 720
GATDADSDES PSSSDTSDEE EGPDRVQCMP S 751 
Gene Ontology
 GO:0005777; C:peroxisome; IEA:InterPro. 
Interpro
 IPR006708; Pex19.
 IPR015943; WD40/YVTN_repeat-like_dom.
 IPR001680; WD40_repeat.
 IPR017986; WD40_repeat_dom. 
Pfam
 PF04614; Pex19
 PF00400; WD40 
SMART
 SM00320; WD40 
PROSITE
 PS50082; WD_REPEATS_2
 PS50294; WD_REPEATS_REGION 
PRINTS