UniProt Accession

 CDK12_RAT; Q3MJK5; Q8R458 

Genbank Protein ID

 AY072294; AY962568 

Genbank Nucleotide ID

 AAL69525.1; AAY41734.1 

Protein Name

 Cyclin-dependent kinase 12 

Protein Synonyms/Alias

 Cdc2-related kinase, arginine/serine-rich; CrkRS; Cell division cycle 2-related protein kinase 7; CDC2-related protein kinase 7; Cell division protein kinase 12; Protein kinase for splicing component 

Gene Name

 Cdk12 

Gene Synonyms/Alias

 Crk7; Crkrs; Pksc 

Created Date

 July 27, 2013 

Organism

 Rattus norvegicus (Rat) 

NCBI Taxa ID

 10116 

Lysine Modification

Position	Peptide	Type	References
501	AQGTKDTKPVALKEV	acetylation	[1]
1464	GPAQSFGKPYRGAAR	acetylation	[1]

Reference

 [1] Proteomic analysis of lysine acetylation sites in rat tissues reveals organ specificity and subcellular patterns.
 Lundby A, Lage K, Weinert BT, Bekker-Jensen DB, Secher A, Skovgaard T, Kelstrup CD, Dmytriyev A, Choudhary C, Lundby C, Olsen JV.
 Cell Rep. 2012 Aug 30;2(2):419-31. [PMID: 22902405] 

Functional Description

 Cyclin-dependent kinase which displays CTD kinase activity and is required for RNA splicing. Has CTD kinase activity by hyperphosphorylating the C-terminal heptapeptide repeat domain (CTD) of the largest RNA polymerase II subunit RPB1, thereby acting as a key regulator of transcription elongation. Involved in regulation of MAP kinase activity, possibly leading to affect the response to estrogn inhibitors (By similarity). Required for RNA splicing, possibly by phosphorylating SRSF1/SF2. 

Sequence Annotation

 DOMAIN 723 1016 Protein kinase.
 NP_BIND 729 737 ATP (By similarity).
 ACT_SITE 855 855 Proton acceptor (By similarity).
 BINDING 752 752 ATP (By similarity).
 MOD_RES 57 57 Phosphothreonine (By similarity).
 MOD_RES 73 73 Phosphotyrosine (By similarity).
 MOD_RES 235 235 Phosphoserine (By similarity).
 MOD_RES 248 248 Phosphoserine (By similarity).
 MOD_RES 273 273 Phosphoserine (By similarity).
 MOD_RES 275 275 Phosphoserine (By similarity).
 MOD_RES 278 278 Phosphotyrosine (By similarity).
 MOD_RES 300 300 Phosphoserine (By similarity).
 MOD_RES 302 302 Phosphoserine (By similarity).
 MOD_RES 309 309 Phosphoserine (By similarity).
 MOD_RES 311 311 Phosphoserine (By similarity).
 MOD_RES 317 317 Phosphoserine (By similarity).
 MOD_RES 318 318 Phosphotyrosine (By similarity).
 MOD_RES 322 322 Phosphoserine (By similarity).
 MOD_RES 324 324 Phosphoserine (By similarity).
 MOD_RES 331 331 Phosphoserine (By similarity).
 MOD_RES 332 332 Phosphoserine (By similarity).
 MOD_RES 333 333 Phosphoserine (By similarity).
 MOD_RES 337 337 Phosphoserine (By similarity).
 MOD_RES 344 344 Phosphoserine (By similarity).
 MOD_RES 382 382 Phosphoserine (By similarity).
 MOD_RES 384 384 Phosphoserine (By similarity).
 MOD_RES 399 399 Phosphoserine (By similarity).
 MOD_RES 419 419 Phosphoserine (By similarity).
 MOD_RES 422 422 Phosphoserine (By similarity).
 MOD_RES 511 511 Phosphothreonine (By similarity).
 MOD_RES 677 677 Phosphoserine (By similarity).
 MOD_RES 681 681 Phosphoserine (By similarity).
 MOD_RES 688 688 Phosphothreonine (By similarity).
 MOD_RES 889 889 Phosphothreonine (By similarity).
 MOD_RES 1049 1049 Phosphoserine (By similarity).
 MOD_RES 1079 1079 Phosphoserine (By similarity).
 MOD_RES 1240 1240 Phosphothreonine (By similarity).  

Keyword

 Alternative splicing; ATP-binding; Complete proteome; Kinase; mRNA processing; mRNA splicing; Nucleotide-binding; Nucleus; Phosphoprotein; Reference proteome; Serine/threonine-protein kinase; Transferase. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 1484 AA 

Protein Sequence

Gene Ontology

 GO:0002944; C:cyclin K-CDK12 complex; IEA:Compara.
 GO:0019908; C:nuclear cyclin-dependent protein kinase holoenzyme complex; IDA:UniProtKB.
 GO:0016607; C:nuclear speck; IDA:UniProtKB.
 GO:0005730; C:nucleolus; IEA:Compara.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0004693; F:cyclin-dependent protein serine/threonine kinase activity; IEA:EC.
 GO:0008353; F:RNA polymerase II carboxy-terminal domain kinase activity; ISS:UniProtKB.
 GO:0006397; P:mRNA processing; IEA:UniProtKB-KW.
 GO:0070816; P:phosphorylation of RNA polymerase II C-terminal domain; ISS:UniProtKB.
 GO:0046777; P:protein autophosphorylation; IEA:Compara.
 GO:0043405; P:regulation of MAP kinase activity; ISS:UniProtKB.
 GO:0008380; P:RNA splicing; IMP:UniProtKB. 

Interpro

 IPR011009; Kinase-like_dom.
 IPR000719; Prot_kinase_cat_dom.
 IPR017441; Protein_kinase_ATP_BS.
 IPR002290; Ser/Thr_dual-sp_kinase_dom.
 IPR008271; Ser/Thr_kinase_AS. 

Pfam

 PF00069; Pkinase 

SMART

 SM00220; S_TKc 

PROSITE

 PS00107; PROTEIN_KINASE_ATP
 PS50011; PROTEIN_KINASE_DOM
 PS00108; PROTEIN_KINASE_ST 

PRINTS