| Tag | Content |
|---|
| CPLM ID | CPLM-015028 |
UniProt Accession | |
Genbank Protein ID | |
Genbank Nucleotide ID | |
Protein Name | DNA ligase |
Protein Synonyms/Alias | Polydeoxyribonucleotide synthase [NAD(+)] |
Gene Name | ligA |
Gene Synonyms/Alias | RPA3517 |
Created Date | July 27, 2013 |
Organism | Rhodopseudomonas palustris (strain ATCC BAA-98 / CGA009) |
NCBI Taxa ID | 258594 |
Lysine Modification | Position | Peptide | Type | References |
|---|
| 225 | AAGSLRQKDVTVTAS | acetylation | [1] |
|
Reference | [1] System-wide studies of N-lysine acetylation in Rhodopseudomonas palustris reveal substrate specificity of protein acetyltransferases. Crosby HA, Pelletier DA, Hurst GB, Escalante-Semerena JC. J Biol Chem. 2012 May 4;287(19):15590-601. [ PMID: 22416131] |
Functional Description | DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of damaged DNA (By similarity). |
Sequence Annotation | DOMAIN 637 715 BRCT.
NP_BIND 47 51 NAD (By similarity).
NP_BIND 96 97 NAD (By similarity).
ACT_SITE 130 130 N6-AMP-lysine intermediate (By
METAL 435 435 Zinc (By similarity).
METAL 438 438 Zinc (By similarity).
METAL 453 453 Zinc (By similarity).
METAL 459 459 Zinc (By similarity).
BINDING 128 128 NAD (By similarity).
BINDING 151 151 NAD (By similarity).
BINDING 188 188 NAD (By similarity).
BINDING 306 306 NAD (By similarity).
BINDING 330 330 NAD (By similarity). |
Keyword | Complete proteome; DNA damage; DNA repair; DNA replication; Ligase; Magnesium; Manganese; Metal-binding; NAD; Zinc. |
Sequence Source | UniProt (SWISSPROT/TrEMBL); GenBank; EMBL |
Protein Length | 715 AA |
Protein Sequence | MTAKTKPAPD IATLTKPKAK VELMRLRLEI EGHDKAYYQD DAPKISDADY DALRRRLEAI 60
EQKFPELVNA SSPTQTVGAA PARGFAKVQH AVPMLSLGNA FADDEVAEFA LRVQRFLKLD 120
DVPAIVAEPK IDGLSLSLRY ENGELVRAAT RGDGFTGEDV TANVRTIKDV PNTLKGKHIP 180
ATCELRGEVY MLKQDFLALN KRQEEANETV FANPRNSAAG SLRQKDVTVT ASRPLKFFAY 240
AWGEMSDYPM EEPTQHKMLQ WLDHAGFVVN PEITLCHSVE DALAFYRRIG EKRASLPYDI 300
DGVVYKVDRL DYQERLGFVS RSPRWAIAHK FAAEQATTVL EKIDIQVGRT GAMTPVARLQ 360
PVTVGGVVVQ NATLHNEDYI KGIGNDGEPI RDGVDIREGD TVVVQRAGDV IPQIVSVVME 420
KRPAGAEPYH FPHKCPVCGS HAVREEGEAV WRCTGALICP AQAVERLKHF VSRLAFDIDG 480
LGEKQIELFH ERGWVQEPAD IFTLKARNAE LKLEQLEGYG ETSVRNLFAA IDARRSIELH 540
RLVFALGIRH VGEGNAKLLA RHYGTLDAFL SAMRAAADAQ TEEGNTSEAY QDLDNIAGIG 600
DVVAEAVVEF FAEERNIKAL DALLAELTEV LPAEQARRDT AVAGKTVVFT GSLSKFTRDE 660
AKAAAERLGA KVAGSVSKKT DYVVAGEDAG SKLTKAKDLG VTVLTEDEWL ALIGN 715 |
Gene Ontology | |
Interpro | |
Pfam | |
SMART | |
PROSITE | |
PRINTS | |