CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-009743
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Ras-related protein Ral-A 
Protein Synonyms/Alias
  
Gene Name
 Rala 
Gene Synonyms/Alias
 Ral; Ral-a 
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
7*MAANKPKGQNSLALubiquitination[1]
134NKSDLEDKRQVSVEEubiquitination[1]
143QVSVEEAKNRADQWNubiquitination[1]
159NYVETSAKTRANVDKubiquitination[1]
Reference
 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023
Functional Description
 Multifunctional GTPase involved in a variety of cellular processes including gene expression, cell migration, cell proliferation, oncogenic transformation and membrane trafficking. Accomplishes its multiple functions by interacting with distinct downstream effectors. Acts as a GTP sensor for GTP-dependent exocytosis of dense core vesicles. Plays a role in the early stages of cytokinesis and is required to tether the exocyst to the cytokinetic furrow. Key regulator of LPAR1 signaling and competes with ADRBK1 for binding to LPAR1 thus affecting the signaling properties of the receptor. Required for anchorage-independent proliferation of transformed cells (By similarity). The RALA- exocyst complex regulates integrin-dependent membrane raft exocytosis and growth signaling. 
Sequence Annotation
 NP_BIND 21 28 GTP (By similarity).
 NP_BIND 68 72 GTP (By similarity).
 NP_BIND 127 130 GTP (By similarity).
 MOTIF 43 51 Effector region (By similarity).
 MOD_RES 203 203 Cysteine methyl ester (By similarity).
 LIPID 203 203 S-geranylgeranyl cysteine (By  
Keyword
 Cell cycle; Cell division; Cell membrane; Complete proteome; Direct protein sequencing; Exocytosis; GTP-binding; Lipoprotein; Membrane; Methylation; Nucleotide-binding; Prenylation; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 206 AA 
Protein Sequence
MAANKPKGQN SLALHKVIMV GSGGVGKSAL TLQFMYDEFV EDYEPTKADS YRKKVVLDGE 60
EVQIDILDTA GQEDYAAIRD NYFRSGEGFL CVFSITEMES FAATADFREQ ILRVKEDENV 120
PFLLVGNKSD LEDKRQVSVE EAKNRADQWN VNYVETSAKT RANVDKVFFD LMREIRARKM 180
EDSKEKNGKK KRKSLAKRIR ERCCIL 206 
Gene Ontology
 GO:0009986; C:cell surface; IEA:UniProtKB-SubCell.
 GO:0032154; C:cleavage furrow; ISS:UniProtKB.
 GO:0030659; C:cytoplasmic vesicle membrane; TAS:Reactome.
 GO:0030139; C:endocytic vesicle; IEA:Compara.
 GO:0030496; C:midbody; IEA:UniProtKB-SubCell.
 GO:0005886; C:plasma membrane; ISS:UniProtKB.
 GO:0031755; F:Edg-2 lysophosphatidic acid receptor binding; ISS:UniProtKB.
 GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
 GO:0003924; F:GTPase activity; IEA:InterPro.
 GO:0031532; P:actin cytoskeleton reorganization; IEA:Compara.
 GO:0000910; P:cytokinesis; ISS:UniProtKB.
 GO:0006887; P:exocytosis; IEA:UniProtKB-KW.
 GO:0051665; P:membrane raft localization; IDA:UniProtKB.
 GO:0051491; P:positive regulation of filopodium assembly; IEA:Compara.
 GO:0017157; P:regulation of exocytosis; IDA:UniProtKB.
 GO:0007264; P:small GTPase mediated signal transduction; IEA:InterPro. 
Interpro
 IPR027417; P-loop_NTPase.
 IPR005225; Small_GTP-bd_dom.
 IPR001806; Small_GTPase.
 IPR020849; Small_GTPase_Ras. 
Pfam
 PF00071; Ras 
SMART
 SM00173; RAS 
PROSITE
 PS51421; RAS 
PRINTS
 PR00449; RASTRNSFRMNG.