CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-001361
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Retinol dehydrogenase 7 
Protein Synonyms/Alias
 Cis-retinol/3alpha-hydroxysterol short-chain dehydrogenase isozyme 2; Cis-retinol/androgen dehydrogenase type 2; CRAD-2 
Gene Name
 Rdh7 
Gene Synonyms/Alias
 Crad2 
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
64LAACLTEKGAEQLRNacetylation[1, 2]
64LAACLTEKGAEQLRNubiquitination[3]
72GAEQLRNKTSDRLETacetylation[1]
72GAEQLRNKTSDRLETubiquitination[3]
98VAATQWVKERVGNRGubiquitination[3]
224RLSHSIEKLWDQTSSacetylation[1, 2, 4, 5]
224RLSHSIEKLWDQTSSubiquitination[3]
234DQTSSEVKEVYDKNFacetylation[1]
234DQTSSEVKEVYDKNFubiquitination[3]
239EVKEVYDKNFLDSYIubiquitination[3]
Reference
 [1] Calorie restriction and SIRT3 trigger global reprogramming of the mitochondrial protein acetylome.
 Hebert AS, Dittenhafer-Reed KE, Yu W, Bailey DJ, Selen ES, Boersma MD, Carson JJ, Tonelli M, Balloon AJ, Higbee AJ, Westphall MS, Pagliarini DJ, Prolla TA, Assadi-Porter F, Roy S, Denu JM, Coon JJ.
 Mol Cell. 2013 Jan 10;49(1):186-99. [PMID: 23201123]
 [2] Quantification of mitochondrial acetylation dynamics highlights prominent sites of metabolic regulation.
 Still AJ, Floyd BJ, Hebert AS, Bingman CA, Carson JJ, Gunderson DR, Dolan BK, Grimsrud PA, Dittenhafer-Reed KE, Stapleton DS, Keller MP, Westphall MS, Denu JM, Attie AD, Coon JJ, Pagliarini DJ.
 J Biol Chem. 2013 Jul 17;. [PMID: 23864654]
 [3] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [4] Circadian acetylome reveals regulation of mitochondrial metabolic pathways.
 Masri S, Patel VR, Eckel-Mahan KL, Peleg S, Forne I, Ladurner AG, Baldi P, Imhof A, Sassone-Corsi P.
 Proc Natl Acad Sci U S A. 2013 Feb 26;110(9):3339-44. [PMID: 23341599]
 [5] Label-free quantitative proteomics of the lysine acetylome in mitochondria identifies substrates of SIRT3 in metabolic pathways.
 Rardin MJ, Newman JC, Held JM, Cusack MP, Sorensen DJ, Li B, Schilling B, Mooney SD, Kahn CR, Verdin E, Gibson BW.
 Proc Natl Acad Sci U S A. 2013 Apr 16;110(16):6601-6. [PMID: 23576753] 
Functional Description
 Acts on androgens and retinols, i.e. has steroid 3- alpha- and 17-beta-dehydrogenase and cis/trans-retinol catalytic activities. 
Sequence Annotation
 NP_BIND 33 57 NADP (By similarity).
 ACT_SITE 175 175 Proton acceptor (By similarity).
 BINDING 163 163 Substrate (By similarity).  
Keyword
 Complete proteome; Endoplasmic reticulum; Microsome; NAD; NADP; Oxidoreductase; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 316 AA 
Protein Sequence
MWLYLVALVG LWTLLRFFRE RQVVSHLQDK YVFITGCDSG FGNLLARQLD RRGMRVLAAC 60
LTEKGAEQLR NKTSDRLETV ILDVTKTESI VAATQWVKER VGNRGLWGLV NNAGICVFAI 120
NEWLKKEDFA NILDVNLLGM IEVTLSMLPL VRKARGRVVN ISSSMGRVSL CGGGYCISKY 180
GVEAFSDSLR REISYFGVKV AIIEPGGFRT NVSNYERLSH SIEKLWDQTS SEVKEVYDKN 240
FLDSYIKAIQ SLTDTCSDDL SVVTDCMEHA LTACHPRTRY SAGWDAKLFY LPLSYMPTFL 300
VDAMLYWSSV KPAQAL 316 
Gene Ontology
 GO:0005783; C:endoplasmic reticulum; IEA:UniProtKB-SubCell.
 GO:0003824; F:catalytic activity; ISS:MGI.
 GO:0004745; F:retinol dehydrogenase activity; IEA:EC.
 GO:0042572; P:retinol metabolic process; IEA:UniProtKB-UniPathway. 
Interpro
 IPR002198; DH_sc/Rdtase_SDR.
 IPR002347; Glc/ribitol_DH.
 IPR016040; NAD(P)-bd_dom.
 IPR020904; Sc_DH/Rdtase_CS. 
Pfam
 PF00106; adh_short 
SMART
  
PROSITE
 PS00061; ADH_SHORT 
PRINTS
 PR00081; GDHRDH.
 PR00080; SDRFAMILY.