CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-002711
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Phosphoenolpyruvate-protein phosphotransferase 
Protein Synonyms/Alias
 Phosphotransferase system, enzyme I 
Gene Name
 ptsI 
Gene Synonyms/Alias
 b2416; JW2409 
Created Date
 July 27, 2013 
Organism
 Escherichia coli (strain K12) 
NCBI Taxa ID
 83333 
Lysine Modification
Position
Peptide
Type
References
20FGKALLLKEDEIVIDacetylation[1]
29DEIVIDRKKISADQVacetylation[1]
30EIVIDRKKISADQVDacetylation[1]
49RFLSGRAKASAQLETacetylation[1]
60QLETIKTKAGETFGEacetylation[1]
96IIALIKDKHMTADAAacetylation[1]
124ELDDEYLKERAADVRacetylation[1]
174ETAQLNLKKVLGFITpupylation[2]
175TAQLNLKKVLGFITDacetylation[3]
238PTNEVIDKMRAVQEQacetylation[1, 3]
250QEQVASEKAELAKLKacetylation[1]
255SEKAELAKLKDLPAIacetylation[1]
340TMDIGGDKELPYMNFacetylation[1]
349LPYMNFPKEENPFLGacetylation[1]
404EEVRALRKEIEIYKQacetylation[1, 3]
410RKEIEIYKQELRDEGacetylation[1]
444TIARHLAKEVDFFSIacetylation[1]
497DASHAEGKWTGMCGEacetylation[1]
546NTNFEDAKVLAEQALacetylation[1]
567ELMTLVNKFIEEKTIacetylation[1]
Reference
 [1] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618]
 [2] Reconstitution of the Mycobacterium tuberculosis pupylation pathway in Escherichia coli.
 Cerda-Maira FA, McAllister F, Bode NJ, Burns KE, Gygi SP, Darwin KH.
 EMBO Rep. 2011 Jul 8;12(8):863-70. [PMID: 21738222]
 [3] Comprehensive profiling of protein lysine acetylation in Escherichia coli.
 Zhang K, Zheng S, Yang JS, Chen Y, Cheng Z.
 J Proteome Res. 2013 Feb 1;12(2):844-51. [PMID: 23294111] 
Functional Description
 General (non sugar-specific) component of the phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS). This major carbohydrate active-transport system catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. Enzyme I transfers the phosphoryl group from phosphoenolpyruvate (PEP) to the phosphoryl carrier protein (HPr). 
Sequence Annotation
 ACT_SITE 189 189 Tele-phosphohistidine intermediate.
 ACT_SITE 502 502 Proton donor (Probable).
 METAL 431 431 Magnesium.
 METAL 455 455 Magnesium.
 BINDING 296 296 Substrate (By similarity).
 BINDING 332 332 Substrate.
 BINDING 431 431 Substrate (By similarity).
 BINDING 452 452 Substrate; via carbonyl oxygen (By
 BINDING 453 453 Substrate; via amide nitrogen (By
 BINDING 454 454 Substrate; via amide nitrogen.
 BINDING 455 455 Substrate; via amide nitrogen.  
Keyword
 3D-structure; Complete proteome; Cytoplasm; Direct protein sequencing; Kinase; Magnesium; Metal-binding; Phosphotransferase system; Reference proteome; Sugar transport; Transferase; Transport. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 575 AA 
Protein Sequence
MISGILASPG IAFGKALLLK EDEIVIDRKK ISADQVDQEV ERFLSGRAKA SAQLETIKTK 60
AGETFGEEKE AIFEGHIMLL EDEELEQEII ALIKDKHMTA DAAAHEVIEG QASALEELDD 120
EYLKERAADV RDIGKRLLRN ILGLKIIDLS AIQDEVILVA ADLTPSETAQ LNLKKVLGFI 180
TDAGGRTSHT SIMARSLELP AIVGTGSVTS QVKNDDYLIL DAVNNQVYVN PTNEVIDKMR 240
AVQEQVASEK AELAKLKDLP AITLDGHQVE VCANIGTVRD VEGAERNGAE GVGLYRTEFL 300
FMDRDALPTE EEQFAAYKAV AEACGSQAVI VRTMDIGGDK ELPYMNFPKE ENPFLGWRAI 360
RIAMDRREIL RDQLRAILRA SAFGKLRIMF PMIISVEEVR ALRKEIEIYK QELRDEGKAF 420
DESIEIGVMV ETPAAATIAR HLAKEVDFFS IGTNDLTQYT LAVDRGNDMI SHLYQPMSPS 480
VLNLIKQVID ASHAEGKWTG MCGELAGDER ATLLLLGMGL DEFSMSAISI PRIKKIIRNT 540
NFEDAKVLAE QALAQPTTDE LMTLVNKFIE EKTIC 575 
Gene Ontology
 GO:0005829; C:cytosol; IDA:UniProtKB.
 GO:0019197; C:phosphoenolpyruvate-dependent sugar phosphotransferase complex; IDA:EcoCyc.
 GO:0016301; F:kinase activity; IEA:UniProtKB-KW.
 GO:0000287; F:magnesium ion binding; IDA:EcoCyc.
 GO:0008965; F:phosphoenolpyruvate-protein phosphotransferase activity; IDA:EcoCyc.
 GO:0005351; F:sugar:hydrogen symporter activity; IEA:InterPro.
 GO:0009401; P:phosphoenolpyruvate-dependent sugar phosphotransferase system; IDA:EcoCyc. 
Interpro
 IPR008279; PEP-util_enz_mobile_dom.
 IPR018274; PEP_util_AS.
 IPR000121; PEP_util_C.
 IPR023151; PEP_util_CS.
 IPR006318; PEP_util_enz.
 IPR024692; PTS_enz_I.
 IPR008731; PTS_PEP_utilis_N.
 IPR015813; Pyrv/PenolPyrv_Kinase-like_dom. 
Pfam
 PF05524; PEP-utilisers_N
 PF00391; PEP-utilizers
 PF02896; PEP-utilizers_C 
SMART
  
PROSITE
 PS00742; PEP_ENZYMES_2
 PS00370; PEP_ENZYMES_PHOS_SITE 
PRINTS
 PR01736; PHPHTRNFRASE.