UniProt Accession

 TGT_HUMAN; Q9BXR0; Q96BQ4; Q9BXQ9 

Genbank Protein ID

 AF302783; AF302784; AC011475; BC015350 

Genbank Nucleotide ID

 AAG60033.1; AAG60034.1; AAH15350.1 

Protein Name

 Queuine tRNA-ribosyltransferase 

Protein Synonyms/Alias

 Guanine insertion enzyme; tRNA-guanine transglycosylase 

Gene Name

 QTRT1 

Gene Synonyms/Alias

 TGT; TGUT 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
55	VGTQATMKGITTEQL	ubiquitination	[1]
219	TCLEEMTKRDVPGFA	ubiquitination	[1, 2, 3]
236	GLSGGESKSQFWRMV	ubiquitination	[1]
308	LRKKVFEKDFGPIDP	ubiquitination	[1]
324	CTCPTCQKHSRAFLH	ubiquitination	[1]

Reference

 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [3] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302] 

Functional Description

 Interacts with QTRTD1 to form an active queuine tRNA- ribosyltransferase. This enzyme exchanges queuine for the guanine at the wobble position of tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr), thereby forming the hypermodified nucleoside queuosine (Q) (7-(((4,5-cis-dihydroxy-2-cyclopenten-1- yl)amino)methyl)-7-deazaguanosine) (By similarity). 

Sequence Annotation

 ACT_SITE 279 279 Nucleophile (By similarity).
 METAL 317 317 Zinc (By similarity).
 METAL 319 319 Zinc (By similarity).
 METAL 322 322 Zinc (By similarity).
 METAL 348 348 Zinc (By similarity).
 BINDING 106 106 Substrate (By similarity).  

Keyword

 Complete proteome; Cytoplasm; Glycosyltransferase; Metal-binding; Mitochondrion; Nucleus; Queuosine biosynthesis; Reference proteome; Transferase; tRNA processing; Zinc. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 403 AA 

Protein Sequence

Gene Ontology

 GO:0005739; C:mitochondrion; IEA:UniProtKB-SubCell.
 GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
 GO:0005840; C:ribosome; NAS:UniProtKB.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0008479; F:queuine tRNA-ribosyltransferase activity; TAS:UniProtKB.
 GO:0008616; P:queuosine biosynthetic process; ISS:UniProtKB. 

Interpro

 IPR004803; Queuine_tRNA-ribosylTrfase.
 IPR002616; tRNA_ribo_trans-like. 

Pfam

 PF01702; TGT 

SMART

  

PROSITE

  

PRINTS