CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-004521
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Zinc finger protein with KRAB and SCAN domains 1 
Protein Synonyms/Alias
 Zinc finger protein 139; Zinc finger protein 36; Zinc finger protein KOX18 
Gene Name
 ZKSCAN1 
Gene Synonyms/Alias
 KOX18; ZNF139; ZNF36 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
280ENEESTSKAETSEDSubiquitination[1, 2]
375EEVPTGTKSHRCDECubiquitination[3]
384HRCDECGKCFTRSSSubiquitination[3]
396SSSLIRHKIIHTGEKubiquitination[3]
459QRIHSGEKPYECNECubiquitination[3]
468YECNECGKAFSQSSDubiquitination[3]
478SQSSDLTKHQRIHTGubiquitination[3]
496YECSECGKAFNRNSYubiquitination[3, 4]
560DAFGAFLKSCV****ubiquitination[3]
Reference
 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [3] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [4] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724] 
Functional Description
 May be involved in transcriptional regulation. 
Sequence Annotation
 DOMAIN 56 138 SCAN box.
 DOMAIN 227 300 KRAB.
 ZN_FING 377 399 C2H2-type 1.
 ZN_FING 405 427 C2H2-type 2.
 ZN_FING 433 455 C2H2-type 3.
 ZN_FING 461 483 C2H2-type 4.
 ZN_FING 489 511 C2H2-type 5.
 ZN_FING 517 539 C2H2-type 6.
 MOD_RES 13 13 Phosphoserine.  
Keyword
 Complete proteome; DNA-binding; Metal-binding; Nucleus; Phosphoprotein; Polymorphism; Reference proteome; Repeat; Transcription; Transcription regulation; Zinc; Zinc-finger. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 563 AA 
Protein Sequence
MMTAESREAT GLSPQAAQEK DGIVIVKVEE EDEEDHMWGQ DSTLQDTPPP DPEIFRQRFR 60
RFCYQNTFGP REALSRLKEL CHQWLRPEIN TKEQILELLV LEQFLSILPK ELQVWLQEYR 120
PDSGEEAVTL LEDLELDLSG QQVPGQVHGP EMLARGMVPL DPVQESSSFD LHHEATQSHF 180
KHSSRKPRLL QSRALPAAHI PAPPHEGSPR DQAMASALFT ADSQAMVKIE DMAVSLILEE 240
WGCQNLARRN LSRDNRQENY GSAFPQGGEN RNENEESTSK AETSEDSASR GETTGRSQKE 300
FGEKRDQEGK TGERQQKNPE EKTRKEKRDS GPAIGKDKKT ITGERGPREK GKGLGRSFSL 360
SSNFTTPEEV PTGTKSHRCD ECGKCFTRSS SLIRHKIIHT GEKPYECSEC GKAFSLNSNL 420
VLHQRIHTGE KPHECNECGK AFSHSSNLIL HQRIHSGEKP YECNECGKAF SQSSDLTKHQ 480
RIHTGEKPYE CSECGKAFNR NSYLILHRRI HTREKPYKCT KCGKAFTRSS TLTLHHRIHA 540
RERASEYSPA SLDAFGAFLK SCV 563 
Gene Ontology
 GO:0005739; C:mitochondrion; IDA:HPA.
 GO:0005634; C:nucleus; NAS:UniProtKB.
 GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
 GO:0003700; F:sequence-specific DNA binding transcription factor activity; NAS:UniProtKB.
 GO:0008270; F:zinc ion binding; IEA:InterPro.
 GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW.
 GO:0016032; P:viral reproduction; IEA:InterPro. 
Interpro
 IPR001909; Krueppel-associated_box.
 IPR008916; Retrov_capsid_C.
 IPR003309; Tscrpt_reg_SCAN.
 IPR007087; Znf_C2H2.
 IPR015880; Znf_C2H2-like.
 IPR013087; Znf_C2H2/integrase_DNA-bd. 
Pfam
 PF01352; KRAB
 PF02023; SCAN 
SMART
 SM00349; KRAB
 SM00431; SCAN
 SM00355; ZnF_C2H2 
PROSITE
 PS50805; KRAB
 PS50804; SCAN_BOX
 PS00028; ZINC_FINGER_C2H2_1
 PS50157; ZINC_FINGER_C2H2_2 
PRINTS