UniProt Accession

 SNF7_YEAST; P39929; D6VY27; E9P8V6 

Genbank Protein ID

 L09751; Z73197; AY558202; BK006945 

Genbank Nucleotide ID

 CAA97548.1; AAS56528.1; DAA09343.1 

Protein Name

 Vacuolar-sorting protein SNF7 

Protein Synonyms/Alias

 DOA4-independent degradation protein 1; Vacuolar protein-sorting-associated protein 32 

Gene Name

 SNF7 

Gene Synonyms/Alias

 DID1; VPS32; YLR025W 

Created Date

 July 27, 2013 

Organism

 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 

NCBI Taxa ID

 559292 

Lysine Modification

Position	Peptide	Type	References
112	RAMQEGAKAMKTIHS	ubiquitination	[1]
196	AAPISENKVSLPSVP	ubiquitination	[1]
229	DEEDEDEKALRELQA	ubiquitination	[1, 2]

Reference

 [1] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301]
 [2] Sites of ubiquitin attachment in Saccharomyces cerevisiae.
 Starita LM, Lo RS, Eng JK, von Haller PD, Fields S.
 Proteomics. 2012 Jan;12(2):236-40. [PMID: 22106047] 

Functional Description

 Required for the sorting and concentration of proteins resulting in the entry of these proteins into the invaginating vesicles of the multivesicular body (MVB). Acts a component of the ESCRT-III complex, which appears to be critical for late steps in MVB sorting, such as membrane invagination and final cargo sorting and recruitment of late-acting components of the sorting machinery. The MVB pathway requires the sequential function of ESCRT-O, -I,-II and -III complex assemblies. Appears to sequester MVB cargo. Recruits BRO1, which in turn recruits DOA4, which deubiquitinates cargos before their enclosure within MVB vesicles. 

Sequence Annotation

 MOD_RES 72 72 Phosphothreonine.
 MOD_RES 119 119 Phosphoserine.
 MOD_RES 193 193 Phosphoserine.  

Keyword

 Complete proteome; Cytoplasm; Endosome; Membrane; Phosphoprotein; Protein transport; Reference proteome; Transport. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 240 AA 

Protein Sequence

Gene Ontology

 GO:0000815; C:ESCRT III complex; IDA:SGD.
 GO:0071454; P:cellular response to anoxia; IMP:SGD.
 GO:0070676; P:intralumenal vesicle formation; IMP:SGD.
 GO:0045324; P:late endosome to vacuole transport; IMP:SGD.
 GO:0015031; P:protein transport; IEA:UniProtKB-KW.
 GO:0043162; P:ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway; IC:SGD. 

Interpro

 IPR005024; Snf7. 

Pfam

 PF03357; Snf7 

SMART

  

PROSITE

  

PRINTS