CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-040975
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
  
Protein Name
 Filamin-A 
Protein Synonyms/Alias
  
Gene Name
 FLNA 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
19AEMPATEKDLAEDAPubiquitination[1]
28LAEDAPWKKIQQNTFacetylation[2, 3]
29AEDAPWKKIQQNTFTubiquitination[1, 4, 5]
44RWCNEHLKCVSKRIAubiquitination[1]
73LLEVLSQKKMHRKHNubiquitination[1, 6]
74LEVLSQKKMHRKHNQubiquitination[1]
113KLVSIDSKAIVDGNLacetylation[3]
206WDSWDASKPVTNAREubiquitination[1]
285EPTGNMVKKRAEFTVacetylation[3]
285EPTGNMVKKRAEFTVubiquitination[6, 7]
341PEVTGTHKVTVLFAGacetylation[8]
353FAGQHIAKSPFEVYVubiquitination[9]
362PFEVYVDKSQGDASKacetylation[3]
490VKETADFKVYTKGAGacetylation[2, 3]
490VKETADFKVYTKGAGubiquitination[9]
494ADFKVYTKGAGSGELacetylation[2]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Proteomic investigations reveal a role for RNA processing factor THRAP3 in the DNA damage response.
 Beli P, Lukashchuk N, Wagner SA, Weinert BT, Olsen JV, Baskcomb L, Mann M, Jackson SP, Choudhary C.
 Mol Cell. 2012 Apr 27;46(2):212-25. [PMID: 22424773]
 [3] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377]
 [4] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [5] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [6] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [7] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [8] Monoclonal antibody cocktail as an enrichment tool for acetylome analysis.
 Shaw PG, Chaerkady R, Zhang Z, Davidson NE, Pandey A.
 Anal Chem. 2011 May 15;83(10):3623-6. [PMID: 21466224]
 [9] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473
Functional Description
  
Sequence Annotation
  
Keyword
 Actin-binding; Complete proteome; Reference proteome; Repeat. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 604 AA 
Protein Sequence
AAPGGGVDTR DAEMPATEKD LAEDAPWKKI QQNTFTRWCN EHLKCVSKRI ANLQTDLSDG 60
LRLIALLEVL SQKKMHRKHN QRPTFRQMQL ENVSVALEFL DRESIKLVSI DSKAIVDGNL 120
KLILGLIWTL ILHYSISMPM WDEEEDEEAK KQTPKQRLLG WIQNKLPQLP ITNFSRDWQS 180
GRALGALVDS CAPGLCPDWD SWDASKPVTN AREAMQQADD WLGIPQVITP EEIVDPNVDE 240
HSVMTYLSQF PKAKLKPGAP LRPKLNPKKA RAYGPGIEPT GNMVKKRAEF TVETRSAGQG 300
EVLVYVEDPA GHQEEAKVTA NNDKNRTFSV WYVPEVTGTH KVTVLFAGQH IAKSPFEVYV 360
DKSQGDASKV TAQGPGLEPS GNIANKTTYF EIFTAGAGTG EVEVVIQDPM GQKGTVEPQL 420
EARGDSTYRC SYQPTMEGVH TVHVTFAGVP IPRSPYTVTV GQACNPSACR AVGRGLQPKG 480
VRVKETADFK VYTKGAGSGE LKVTVKGPRR GAREAEGPGG WRVWLRVLPH GPWNLYRHHH 540
VGWSEHRAQS LRSEGGHRVW QSEGTGLGPW AGGRRRWQVS RLCGGGYRGR RGHAGLLGGR 600
AIAG 604 
Gene Ontology
  
Interpro
 IPR001589; Actinin_actin-bd_CS.
 IPR001715; CH-domain.
 IPR001298; Filamin.
 IPR017868; Filamin/ABP280_repeat-like.
 IPR013783; Ig-like_fold.
 IPR014756; Ig_E-set. 
Pfam
 PF00307; CH
 PF00630; Filamin 
SMART
 SM00033; CH
 SM00557; IG_FLMN 
PROSITE
 PS00019; ACTININ_1
 PS00020; ACTININ_2
 PS50021; CH
 PS50194; FILAMIN_REPEAT 
PRINTS