UniProt Accession

 SNX18_HUMAN; Q96RF0; B4E2B3; H7BXX3; Q05BB3; Q0VG02 

Genbank Protein ID

 AF395536; AK304195; AC091888; CH471123; BC060791; BC067860; BC117218; BC117220 

Genbank Nucleotide ID

 AAK82415.1; BAG65075.1; EAW54894.1; AAH60791.1; AAH67860.1; AAI17219.1; AAI17221.1 

Protein Name

 Sorting nexin-18 

Protein Synonyms/Alias

 SH3 and PX domain-containing protein 3B; Sorting nexin-associated Golgi protein 1 

Gene Name

 SNX18 

Gene Synonyms/Alias

 SH3PXD3B; SNAG1 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
253	GEASGFVKDGDKLCV	ubiquitination	[1]
314	VPVHRRYKHFDWLYA	ubiquitination	[2]
326	LYARLAEKFPVISVP	ubiquitination	[1, 2, 3]
338	SVPHLPEKQATGRFE	ubiquitination	[1, 2]
432	ESKIDGFKCFTKKMD	ubiquitination	[2]
437	GFKCFTKKMDDSALQ	ubiquitination	[2]
466	GFKKEYQKVGQSFRG	ubiquitination	[2]

Reference

 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302] 

Functional Description

 May be involved in several stages of intracellular trafficking. Plays a role in endocytosis via clathrin-coated pits, but also clathrin-independent, actin-dependent fluid-phase endocytosis. Plays a role in macropinocytosis. Binds to membranes enriched in phosphatidylinositol 4,5-bisphosphate and promotes membrane tubulation. Stimulates the GTPase activity of DNM2. Promotes DNM2 location at the plasma membrane. 

Sequence Annotation

 DOMAIN 1 61 SH3.
 DOMAIN 276 386 PX.
 DOMAIN 421 628 BAR.
 BINDING 312 312 Phosphatidylinositol 4,5-bisphosphate (By
 BINDING 314 314 Phosphatidylinositol 4,5-bisphosphate (By
 BINDING 338 338 Phosphatidylinositol 4,5-bisphosphate (By
 BINDING 352 352 Phosphatidylinositol 4,5-bisphosphate (By  

Keyword

 Alternative splicing; Cell membrane; Complete proteome; Endocytosis; Endosome; Lipid-binding; Membrane; Polymorphism; Protein transport; Reference proteome; SH3 domain; Transport. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 628 AA 

Protein Sequence

Gene Ontology

 GO:0012505; C:endomembrane system; IEA:UniProtKB-SubCell.
 GO:0010008; C:endosome membrane; IEA:UniProtKB-SubCell.
 GO:0031234; C:extrinsic to internal side of plasma membrane; IDA:UniProtKB.
 GO:0035091; F:phosphatidylinositol binding; IEA:InterPro.
 GO:0007154; P:cell communication; IEA:InterPro.
 GO:0006897; P:endocytosis; IDA:UniProtKB.
 GO:0043547; P:positive regulation of GTPase activity; IDA:UniProtKB.
 GO:0015031; P:protein transport; IEA:UniProtKB-KW. 

Interpro

 IPR001683; Phox.
 IPR011511; SH3_2.
 IPR001452; SH3_domain.
 IPR014536; Snx9.
 IPR019497; Sorting_nexin_WASP-bd-dom. 

Pfam

 PF10456; BAR_3_WASP_bdg
 PF00787; PX
 PF07653; SH3_2 

SMART

 SM00312; PX
 SM00326; SH3 

PROSITE

 PS51021; BAR
 PS50195; PX
 PS50002; SH3 

PRINTS