UniProt Accession

 PKHF1_HUMAN; Q96S99; Q96K11; Q9BUB9 

Genbank Protein ID

 AY037145; AF434818; AK027758; BC002744 

Genbank Nucleotide ID

 AAK67626.2; AAL30773.1; BAB55349.1; AAH02744.1 

Protein Name

 Pleckstrin homology domain-containing family F member 1 

Protein Synonyms/Alias

 PH domain-containing family F member 1; Lysosome-associated apoptosis-inducing protein containing PH and FYVE domains; Apoptosis-inducing protein; PH and FYVE domain-containing protein 1; Phafin-1; Zinc finger FYVE domain-containing protein 15 

Gene Name

 PLEKHF1 

Gene Synonyms/Alias

 APPD; LAPF; ZFYVE15 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
44	LGEGVLTKECRKKAK	acetylation	[1]
44	LGEGVLTKECRKKAK	ubiquitination	[2]

Reference

 [1] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961] 

Functional Description

 May induce apoptosis through the lysosomal-mitochondrial pathway. Translocates to the lysosome initiating the permeabilization of lysosomal membrane (LMP) and resulting in the release of CTSD and CTSL to the cytoplasm. Triggers the caspase- independent apoptosis by altering mitochondrial membrane permeabilization (MMP) resulting in the release of PDCD8. 

Sequence Annotation

 DOMAIN 35 131 PH.
 ZN_FING 152 212 FYVE-type.  

Keyword

 Apoptosis; Complete proteome; Cytoplasm; Lysosome; Metal-binding; Nucleus; Reference proteome; Zinc; Zinc-finger. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 279 AA 

Protein Sequence

Gene Ontology

 GO:0010008; C:endosome membrane; IC:BHF-UCL.
 GO:0005765; C:lysosomal membrane; IC:BHF-UCL.
 GO:0005634; C:nucleus; IDA:HPA.
 GO:0048471; C:perinuclear region of cytoplasm; IEA:UniProtKB-SubCell.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0032266; F:phosphatidylinositol-3-phosphate binding; IDA:BHF-UCL.
 GO:0070273; F:phosphatidylinositol-4-phosphate binding; IDA:BHF-UCL.
 GO:0010314; F:phosphatidylinositol-5-phosphate binding; IDA:BHF-UCL.
 GO:0006915; P:apoptotic process; IEA:UniProtKB-KW.
 GO:0007032; P:endosome organization; IMP:BHF-UCL.
 GO:0006917; P:induction of apoptosis; IEA:Compara.
 GO:0010508; P:positive regulation of autophagy; IDA:BHF-UCL.
 GO:0072659; P:protein localization to plasma membrane; IMP:BHF-UCL.
 GO:0016050; P:vesicle organization; IMP:BHF-UCL. 

Interpro

 IPR011993; PH_like_dom.
 IPR001849; Pleckstrin_homology.
 IPR000306; Znf_FYVE.
 IPR017455; Znf_FYVE-rel.
 IPR011011; Znf_FYVE_PHD.
 IPR013083; Znf_RING/FYVE/PHD. 

Pfam

 PF01363; FYVE
 PF00169; PH 

SMART

 SM00064; FYVE
 SM00233; PH 

PROSITE

 PS50003; PH_DOMAIN
 PS50178; ZF_FYVE 

PRINTS