UniProt Accession

 LPXD_ECOLI; P21645 

Genbank Protein ID

 X54797; U70214; U00096; AP009048 

Genbank Nucleotide ID

 CAA38568.1; AAB08608.1; AAC73290.1; BAA77854.1 

Protein Name

 UDP-3-O-(3-hydroxymyristoyl)glucosamine N-acyltransferase 

Protein Synonyms/Alias

 UDP-3-O-(3-OHC14)-GlcN N-acyltransferase; Protein FirA; Rifampicin resistance protein 

Gene Name

 lpxD 

Gene Synonyms/Alias

 firA; omsA; b0179; JW0174 

Created Date

 July 27, 2013 

Organism

 Escherichia coli (strain K12) 

NCBI Taxa ID

 83333 

Lysine Modification

Position	Peptide	Type	References
77	KSAALVVKNPYLTYA	acetylation	[1, 2]
311	GIPLQPNKVWRKTAA	acetylation	[2]
328	MNIDDMSKRLKSLER	acetylation	[2]

Reference

 [1] Comprehensive profiling of protein lysine acetylation in Escherichia coli.
 Zhang K, Zheng S, Yang JS, Chen Y, Cheng Z.
 J Proteome Res. 2013 Feb 1;12(2):844-51. [PMID: 23294111]
 [2] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618] 

Functional Description

 Catalyzes the N-acylation of UDP-3-O- (hydroxymyristoyl)glucosamine using 3-hydroxymyristoyl-ACP as the acyl donor. Is involved in the biosynthesis of lipid A, a phosphorylated glycolipid that anchors the lipopolysaccharide to the outer membrane of the cell. Prefers R-3-hydroxymyristoyl-ACP over R-3-hydroxypalmitoyl-ACP as the acyl donor in vitro, which is consistent with the structure of E.coli lipid A that contains over 95% (R)-3-hydroxymyristate at the 2 and 2' positions. 

Sequence Annotation

 ACT_SITE 239 239 Proton acceptor.  

Keyword

 3D-structure; Acyltransferase; Antibiotic resistance; Complete proteome; Direct protein sequencing; Lipid A biosynthesis; Lipid biosynthesis; Lipid metabolism; Reference proteome; Repeat; Transferase. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 341 AA 

Protein Sequence

Gene Ontology

 GO:0016410; F:N-acyltransferase activity; IDA:EcoliWiki.
 GO:0009245; P:lipid A biosynthetic process; IMP:EcoliWiki.
 GO:0046677; P:response to antibiotic; IEA:UniProtKB-KW. 

Interpro

 IPR001451; Hexapep_transf.
 IPR018357; Hexapep_transf_CS.
 IPR011004; Trimer_LpxA-like.
 IPR007691; UDP-3-O_GlcNAc_AcTrfase.
 IPR020573; UDP_GlcNAc_AcTrfase_non-rep. 

Pfam

 PF00132; Hexapep
 PF04613; LpxD 

SMART

  

PROSITE

 PS00101; HEXAPEP_TRANSFERASES 

PRINTS