UniProt Accession

 DOHH_YEAST; P47120; D6VWP1 

Genbank Protein ID

 Z49570; L47993; BK006943 

Genbank Nucleotide ID

 CAA89598.1; AAB39296.1; DAA08857.1 

Protein Name

 Deoxyhypusine hydroxylase 

Protein Synonyms/Alias

 DOHH; Deoxyhypusine dioxygenase; Deoxyhypusine monooxygenase; Ligand of eIF5A protein 1 

Gene Name

 LIA1 

Gene Synonyms/Alias

 MMD1; YJR070C; J1814 

Created Date

 July 27, 2013 

Organism

 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 

NCBI Taxa ID

 559292 

Lysine Modification

Position	Peptide	Type	References
124	ALGALGDKDSLDDLN	acetylation	[1]
161	NWTHGGAKDKENLQQ	acetylation	[1]

Reference

 [1] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919] 

Functional Description

 Catalyzes the hydroxylation of the N(6)-(4-aminobutyl)- L-lysine intermediate to form hypusine, an essential post- translational modification only found in mature eIF-5A factor (By similarity). 

Sequence Annotation

 REPEAT 77 103 HEAT-like PBS-type 1.
 REPEAT 110 136 HEAT-like PBS-type 2.
 REPEAT 202 231 HEAT-like PBS-type 3.
 REPEAT 235 261 HEAT-like PBS-type 4.
 REPEAT 268 294 HEAT-like PBS-type 5.
 METAL 79 79 Iron 1.
 METAL 80 80 Iron 1.
 METAL 112 112 Iron 1.
 METAL 113 113 Iron 1.
 METAL 237 237 Iron 2.
 METAL 238 238 Iron 2.
 METAL 270 270 Iron 2.
 METAL 271 271 Iron 2.
 MOD_RES 2 2 N-acetylserine.
 MOD_RES 126 126 Phosphoserine.
 MOD_RES 187 187 Phosphothreonine.
 MOD_RES 281 281 Phosphoserine.  

Keyword

 Acetylation; Complete proteome; Cytoplasm; Hypusine biosynthesis; Iron; Metal-binding; Monooxygenase; Nucleus; Oxidoreductase; Phosphoprotein; Reference proteome; Repeat. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 325 AA 

Protein Sequence

Gene Ontology

 GO:0005737; C:cytoplasm; IDA:SGD.
 GO:0005634; C:nucleus; IDA:SGD.
 GO:0019135; F:deoxyhypusine monooxygenase activity; IDA:SGD.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0000226; P:microtubule cytoskeleton organization; IGI:SGD.
 GO:0008612; P:peptidyl-lysine modification to hypusine; IDA:SGD. 

Interpro

 IPR011989; ARM-like.
 IPR016024; ARM-type_fold.
 IPR027517; Deoxyhypusine_hydroxylase.
 IPR004155; PBS_lyase_HEAT. 

Pfam

 PF03130; HEAT_PBS 

SMART

 SM00567; EZ_HEAT 

PROSITE

 PS50077; HEAT_REPEAT 

PRINTS