UniProt Accession

 DPH5_YEAST; P32469; D6VYH6; Q6Q5L4 

Genbank Protein ID

 M83375; U17246; AY557947; X70279; BK006945 

Genbank Nucleotide ID

 AAA34577.1; AAB67469.1; AAS56273.1; CAA49764.1; DAA09492.1 

Protein Name

 Diphthine synthase 

Protein Synonyms/Alias

 Diphthamide biosynthesis methyltransferase 

Gene Name

 DPH5 

Gene Synonyms/Alias

 YLR172C; L9470.17 

Created Date

 July 27, 2013 

Organism

 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 

NCBI Taxa ID

 559292 

Lysine Modification

Position	Peptide	Type	References
208	IEEKRGTKAYTPDTP	ubiquitination	[1]

Reference

 [1] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301] 

Functional Description

 S-adenosyl-L-methionine-dependent methyltransferase that catalyzes the trimethylation of the amino group of the modified target histidine residue in translation elongation factor 2 (EF- 2), to form an intermediate called diphthine. The three successive methylation reactions represent the second step of diphthamide biosynthesis. 

Sequence Annotation

 REGION 113 114 S-adenosyl-L-methionine binding (By
 BINDING 9 9 S-adenosyl-L-methionine; via carbonyl
 BINDING 85 85 S-adenosyl-L-methionine; via amide
 BINDING 88 88 S-adenosyl-L-methionine; via carbonyl
 BINDING 164 164 S-adenosyl-L-methionine; via amide
 BINDING 222 222 S-adenosyl-L-methionine; via amide
 BINDING 247 247 S-adenosyl-L-methionine; via carbonyl
 MOD_RES 172 172 Phosphoserine.
 MOD_RES 298 298 Phosphoserine.  

Keyword

 Complete proteome; Cytoplasm; Methyltransferase; Phosphoprotein; Reference proteome; S-adenosyl-L-methionine; Transferase. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 300 AA 

Protein Sequence

Gene Ontology

 GO:0005737; C:cytoplasm; IDA:SGD.
 GO:0004164; F:diphthine synthase activity; IDA:SGD.
 GO:0017183; P:peptidyl-diphthamide biosynthetic process from peptidyl-histidine; IDA:SGD. 

Interpro

 IPR000878; 4pyrrol_Mease.
 IPR014777; 4pyrrole_Mease_sub1.
 IPR014776; 4pyrrole_Mease_sub2.
 IPR004551; Dphthn_synthase. 

Pfam

 PF00590; TP_methylase 

SMART

  

PROSITE

  

PRINTS