CPLM-012540

Genbank Nucleotide ID

Transmembrane emp24 domain-containing protein 2

Protein Synonyms/Alias

Membrane protein p24A; p24; p24 family protein beta-1; p24beta1

Homo sapiens (Human)

Position	Peptide	Type	References
71	PDNKGIYKGDRESSG	ubiquitination	[1]

[1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]

Functional Description

Involved in vesicular protein trafficking. Mainly functions in the early secretory pathway but also in post-Golgi membranes. Thought to act as cargo receptor at the lumenal side for incorporation of secretory cargo molecules into transport vesicles and to be involved in vesicle coat formation at the cytoplasmic side. In COPII vesicle-mediated anterograde transport involved in the transport of GPI-anchored proteins and proposed to act togther with TMED10 as their cargo receptor; the function specifically implies SEC24C and SEC24D of the COPII vesicle coat and lipid raft-like microdomains of the ER. Recognizes GPI anchors structural remodeled in the ER by PGAP1 and MPPE1. In COPI vesicle-mediated retrograde transport inhibits the GTPase- activating activity of ARFGAP1 towards ARF1 thus preventing immature uncoating and allowing cargo selection to take place. Involved in trafficking of G protein-coupled receptors (GPCRs). Regulates F2RL1, OPRM1 and P2RY4 exocytic trafficking from the Golgi to the plasma membrane thus contributing to receptor resensitization. Facilitates CASR maturation and stabilization in the early secretory pathway and increases CASR plasma membrane targeting. Proposed to be involved in organization of intracellular membranes such as the maintenance of the Golgi apparatus. May also play a role in the biosynthesis of secreted cargo such as eventual processing.

DOMAIN 30 112 GOLD.
REGION 1 181 Interaction with F2RL1.
REGION 118 157 Required for TMED10 and TMED2 cis-Golgi
MOTIF 194 201 COPI vesicle coat-binding (Potential).
MOTIF 194 195 COPII vesicle coat-binding (Potential).

Coiled coil; Complete proteome; Cytoplasmic vesicle; Endoplasmic reticulum; ER-Golgi transport; Golgi apparatus; Membrane; Protein transport; Reference proteome; Signal; Transmembrane; Transmembrane helix; Transport.

UniProt (SWISSPROT/TrEMBL); GenBank; EMBL

GO:0030137; C:COPI-coated vesicle; ISS:UniProtKB.
GO:0030663; C:COPI-coated vesicle membrane; IEA:UniProtKB-SubCell.
GO:0005783; C:endoplasmic reticulum; IDA:UniProtKB.
GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
GO:0005793; C:endoplasmic reticulum-Golgi intermediate compartment; IDA:UniProtKB.
GO:0033116; C:endoplasmic reticulum-Golgi intermediate compartment membrane; IEA:UniProtKB-SubCell.
GO:0032580; C:Golgi cisterna membrane; IEA:UniProtKB-SubCell.
GO:0016021; C:integral to membrane; ISS:HGNC.
GO:0042589; C:zymogen granule membrane; ISS:HGNC.
GO:0035459; P:cargo loading into vesicle; TAS:UniProtKB.
GO:0048205; P:COPI coating of Golgi vesicle; TAS:UniProtKB.
GO:0048208; P:COPII vesicle coating; TAS:UniProtKB.
GO:0001892; P:embryonic placenta development; IEA:Compara.
GO:0007030; P:Golgi organization; IMP:UniProtKB.
GO:0001947; P:heart looping; IEA:Compara.
GO:0001893; P:maternal placenta development; IEA:Compara.
GO:0035264; P:multicellular organism growth; IEA:Compara.
GO:0034260; P:negative regulation of GTPase activity; IDA:UniProtKB.
GO:0001843; P:neural tube closure; IEA:Compara.
GO:0010628; P:positive regulation of gene expression; IEA:Compara.
GO:0036342; P:post-anal tail morphogenesis; IEA:Compara.
GO:0072661; P:protein targeting to plasma membrane; IDA:UniProtKB.
GO:0032525; P:somite rostral/caudal axis specification; IEA:Compara.

PF01105; EMP24_GP25L