UniProt Accession

 PAT1_YEAST; P25644; D6VR80; Q8NKJ3 

Genbank Protein ID

 X59720; BK006937 

Genbank Nucleotide ID

 CAC42990.1; DAA07549.2 

Protein Name

 DNA topoisomerase 2-associated protein PAT1 

Protein Synonyms/Alias

 Decapping activator and translational repressor PAT1; Topoisomerase II-associated protein PAT1; mRNA turnover protein 1 

Gene Name

 PAT1 

Gene Synonyms/Alias

 MRT1; YCR077C; YCR77C 

Created Date

 July 27, 2013 

Organism

 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 

NCBI Taxa ID

 559292 

Lysine Modification

Position	Peptide	Type	References
324	GITSESNKGLIARAY	ubiquitination	[1]
372	KERPSKLKDQQAAAG	ubiquitination	[1]
386	GNSSQDNKQANTVLG	ubiquitination	[1]

Reference

 [1] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301] 

Functional Description

 Activator of decapping that functions as a general and active mechanism of translational repression and required for P- body formation. First decay factor recruited to mRNA, at a time when the mRNA is still associated with translation factors. Subsequently, PAT1 recruits the hepta-heterodimer LSM1-LSM7 complex to P-bodies. In association with the LSM1-LSM7 complex, stabilizes the 3' terminus of mRNAs. This association is also required for mosaic virus genomic RNA translation. Modulates the rates of mRNA-decapping that occur following deadenylation. Might be required for promoting the formation or the stabilization of the preinitiation translation complexes. Required for 40S ribosomal subunit joining to capped and/or polyadenylated mRNA. With other P-body components, enhances the formation of retrotransposition-competent Ty1 virus-like particles. Necessary for accurate chromosome transmission during cell division. 

Sequence Annotation

 MOD_RES 456 456 Phosphoserine.
 MOD_RES 457 457 Phosphoserine.  

Keyword

 Cell cycle; Cell division; Complete proteome; Cytoplasm; mRNA processing; Nucleus; Phosphoprotein; Reference proteome; Repressor; RNA-binding; Translation regulation. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 796 AA 

Protein Sequence

Gene Ontology

 GO:0000932; C:cytoplasmic mRNA processing body; IDA:SGD.
 GO:0022627; C:cytosolic small ribosomal subunit; IDA:SGD.
 GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
 GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
 GO:0007049; P:cell cycle; IEA:UniProtKB-KW.
 GO:0051301; P:cell division; IEA:UniProtKB-KW.
 GO:0033962; P:cytoplasmic mRNA processing body assembly; IMP:SGD.
 GO:0000290; P:deadenylation-dependent decapping of nuclear-transcribed mRNA; IMP:SGD.
 GO:0001731; P:formation of translation preinitiation complex; IMP:SGD.
 GO:0006397; P:mRNA processing; IEA:UniProtKB-KW.
 GO:0045947; P:negative regulation of translational initiation; IDA:SGD. 

Interpro

 IPR019167; Topo_II-assoc_PAT1. 

Pfam

 PF09770; PAT1 

SMART

  

PROSITE

  

PRINTS