UniProt Accession

 PGK_ECOLI; P0A799; P11665; Q2M9R6 

Genbank Protein ID

 X14436; U28377; U00096; AP009048 

Genbank Nucleotide ID

 CAA32604.1; AAA69093.1; AAC75963.1; BAE76990.1 

Protein Name

 Phosphoglycerate kinase 

Protein Synonyms/Alias

  

Gene Name

 pgk 

Gene Synonyms/Alias

 b2926; JW2893 

Created Date

 July 27, 2013 

Organism

 Escherichia coli (strain K12) 

NCBI Taxa ID

 83333 

Lysine Modification

Position	Peptide	Type	References
5	***MSVIKMTDLDLA	acetylation	[1, 2]
14	TDLDLAGKRVFIRAD	acetylation	[1, 2]
27	ADLNVPVKDGKVTSD	acetylation	[1, 2]
30	NVPVKDGKVTSDARI	acetylation	[1, 2]
49	PTIELALKQGAKVMV	acetylation	[1, 2]
82	LPVVNYLKDKLSNPV	acetylation	[1, 2]
84	VVNYLKDKLSNPVRL	acetylation	[1, 2, 3]
93	SNPVRLVKDYLDGVD	acetylation	[1, 2]
116	LENVRFNKGEKKDDE	acetylation	[2, 4]
119	VRFNKGEKKDDETLS	acetylation	[2]
120	RFNKGEKKDDETLSK	acetylation	[1, 2]
127	KDDETLSKKYAALCD	acetylation	[2]
128	DDETLSKKYAALCDV	acetylation	[1]
156	ASTHGIGKFADVACA	acetylation	[1, 2]
176	AELDALGKALKEPAR	acetylation	[1, 2]
179	DALGKALKEPARPMV	acetylation	[1, 2]
193	VAIVGGSKVSTKLTV	acetylation	[2]
197	GGSKVSTKLTVLDSL	acetylation	[1, 2]
206	TVLDSLSKIADQLIV	acetylation	[1]
243	ADLVDEAKRLLTTCN	acetylation	[1, 2]
272	ETAPATLKSVNDVKA	acetylation	[1, 2]
278	LKSVNDVKADEQILD	acetylation	[1, 2]
299	QELAEILKNAKTILW	acetylation	[1, 2]
302	AEILKNAKTILWNGP	acetylation	[1, 2]
373	FLEFVEGKVLPAVAM	acetylation	[1]

Reference

 [1] Comprehensive profiling of protein lysine acetylation in Escherichia coli.
 Zhang K, Zheng S, Yang JS, Chen Y, Cheng Z.
 J Proteome Res. 2013 Feb 1;12(2):844-51. [PMID: 23294111]
 [2] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618]
 [3] Lysine acetylation is a highly abundant and evolutionarily conserved modification in Escherichia coli.
 Zhang J, Sprung R, Pei J, Tan X, Kim S, Zhu H, Liu CF, Grishin NV, Zhao Y.
 Mol Cell Proteomics. 2009 Feb;8(2):215-25. [PMID: 18723842]
 [4] The diversity of lysine-acetylated proteins in Escherichia coli.
 Yu BJ, Kim JA, Moon JH, Ryu SE, Pan JG.
 J Microbiol Biotechnol. 2008 Sep;18(9):1529-36. [PMID: 18852508] 

Functional Description

  

Sequence Annotation

 NP_BIND 340 343 ATP (By similarity).
 REGION 21 23 Substrate binding (By similarity).
 REGION 59 62 Substrate binding (By similarity).
 BINDING 36 36 Substrate (By similarity).
 BINDING 113 113 Substrate (By similarity).
 BINDING 146 146 Substrate (By similarity).
 BINDING 197 197 ATP (By similarity).
 BINDING 314 314 ATP (By similarity).
 MOD_RES 84 84 N6-acetyllysine.  

Keyword

 3D-structure; Acetylation; ATP-binding; Complete proteome; Cytoplasm; Direct protein sequencing; Glycolysis; Kinase; Nucleotide-binding; Reference proteome; Transferase. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 387 AA 

Protein Sequence

Gene Ontology

 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0004618; F:phosphoglycerate kinase activity; IEA:HAMAP.
 GO:0006096; P:glycolysis; IEA:HAMAP. 

Interpro

 IPR001576; Phosphoglycerate_kinase.
 IPR015901; Phosphoglycerate_kinase_C.
 IPR015911; Phosphoglycerate_kinase_CS.
 IPR015824; Phosphoglycerate_kinase_N. 

Pfam

 PF00162; PGK 

SMART

  

PROSITE

 PS00111; PGLYCERATE_KINASE 

PRINTS

 PR00477; PHGLYCKINASE.