CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-017128
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Chondroitin sulfate synthase 2 
Protein Synonyms/Alias
 Chondroitin glucuronyltransferase 2; Chondroitin-polymerizing factor; ChPF; Glucuronosyl-N-acetylgalactosaminyl-proteoglycan 4-beta-N-acetylgalactosaminyltransferase II; N-acetylgalactosaminyl-proteoglycan 3-beta-glucuronosyltransferase II; N-acetylgalactosaminyltransferase 2 
Gene Name
 CHPF 
Gene Synonyms/Alias
 CSS2; UNQ651/PRO1281 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
73QPGAEREKPGAGEGAubiquitination[1, 2]
101AQPGQAAKKAVRTRYubiquitination[1, 2]
338VHMYQLHKAFARAELubiquitination[1, 2]
445HPALRLQKQQLVNGYubiquitination[1, 2, 3]
567ADVFAPVKAHVAELEubiquitination[1, 2]
603RLMDLLSKKHPLDTLubiquitination[1, 2]
604LMDLLSKKHPLDTLFubiquitination[1, 2]
Reference
 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [3] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961] 
Functional Description
 Has both beta-1,3-glucuronic acid and beta-1,4-N- acetylgalactosamine transferase activity. Transfers glucuronic acid (GlcUA) from UDP-GlcUA and N-acetylgalactosamine (GalNAc) from UDP-GalNAc to the non-reducing end of the elongating chondroitin polymer. 
Sequence Annotation
 METAL 617 617 Divalent metal cation (Potential).
 CARBOHYD 138 138 N-linked (GlcNAc...) (Potential).
 CARBOHYD 361 361 N-linked (GlcNAc...) (Potential).  
Keyword
 Complete proteome; Glycoprotein; Golgi apparatus; Membrane; Metal-binding; Polymorphism; Reference proteome; Signal-anchor; Transferase; Transmembrane; Transmembrane helix. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 775 AA 
Protein Sequence
MRASLLLSVL RPAGPVAVGI SLGFTLSLLS VTWVEEPCGP GPPQPGDSEL PPRGNTNAAR 60
RPNSVQPGAE REKPGAGEGA GENWEPRVLP YHPAQPGQAA KKAVRTRYIS TELGIRQRLL 120
VAVLTSQTTL PTLGVAVNRT LGHRLERVVF LTGARGRRAP PGMAVVTLGE ERPIGHLHLA 180
LRHLLEQHGD DFDWFFLVPD TTYTEAHGLA RLTGHLSLAS AAHLYLGRPQ DFIGGEPTPG 240
RYCHGGFGVL LSRMLLQQLR PHLEGCRNDI VSARPDEWLG RCILDATGVG CTGDHEGVHY 300
SHLELSPGEP VQEGDPHFRS ALTAHPVRDP VHMYQLHKAF ARAELERTYQ EIQELQWEIQ 360
NTSHLAVDGD QAAAWPVGIP APSRPASRFE VLRWDYFTEQ HAFSCADGSP RCPLRGADRA 420
DVADVLGTAL EELNRRYHPA LRLQKQQLVN GYRRFDPARG MEYTLDLQLE ALTPQGGRRP 480
LTRRVQLLRP LSRVEILPVP YVTEASRLTV LLPLAAAERD LAPGFLEAFA TAALEPGDAA 540
AALTLLLLYE PRQAQRVAHA DVFAPVKAHV AELERRFPGA RVPWLSVQTA APSPLRLMDL 600
LSKKHPLDTL FLLAGPDTVL TPDFLNRCRM HAISGWQAFF PMHFQAFHPA VAPPQGPGPP 660
ELGRDTGRFD RQAASEACFY NSDYVAARGR LAAASEQEEE LLESLDVYEL FLHFSSLHVL 720
RAVEPALLQR YRAQTCSARL SEDLYHRCLQ SVLEGLGSRT QLAMLLFEQE QGNST 775 
Gene Ontology
 GO:0032580; C:Golgi cisterna membrane; IEA:UniProtKB-SubCell.
 GO:0000139; C:Golgi membrane; TAS:Reactome.
 GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
 GO:0047238; F:glucuronosyl-N-acetylgalactosaminyl-proteoglycan 4-beta-N-acetylgalactosaminyltransferase activity; IEA:EC.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0050510; F:N-acetylgalactosaminyl-proteoglycan 3-beta-glucuronosyltransferase activity; IEA:EC.
 GO:0005975; P:carbohydrate metabolic process; TAS:Reactome.
 GO:0030206; P:chondroitin sulfate biosynthetic process; TAS:Reactome. 
Interpro
 IPR008428; Chond_GalNAc. 
Pfam
 PF05679; CHGN 
SMART
  
PROSITE
  
PRINTS