CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-023386
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Pre-mRNA-splicing factor CWC22 homolog 
Protein Synonyms/Alias
 Nucampholin 
Gene Name
 ncm 
Gene Synonyms/Alias
 CG12750 
Created Date
 July 27, 2013 
Organism
 Drosophila melanogaster (Fruit fly) 
NCBI Taxa ID
 7227 
Lysine Modification
Position
Peptide
Type
References
654NYAENEDKYKGLSREacetylation[1]
Reference
 [1] Proteome-wide mapping of the Drosophila acetylome demonstrates a high degree of conservation of lysine acetylation.
 Weinert BT, Wagner SA, Horn H, Henriksen P, Liu WR, Olsen JV, Jensen LJ, Choudhary C.
 Sci Signal. 2011 Jul 26;4(183):ra48. [PMID: 21791702
Functional Description
 May be involved in pre-mRNA splicing. 
Sequence Annotation
 DOMAIN 420 603 MIF4G.
 DOMAIN 710 826 MI.
 MOD_RES 191 191 Phosphothreonine.
 MOD_RES 201 201 Phosphothreonine.
 MOD_RES 219 219 Phosphoserine.
 MOD_RES 221 221 Phosphoserine.
 MOD_RES 1108 1108 Phosphothreonine.
 MOD_RES 1111 1111 Phosphoserine.
 MOD_RES 1121 1121 Phosphoserine.
 MOD_RES 1180 1180 Phosphoserine.
 MOD_RES 1181 1181 Phosphoserine.
 MOD_RES 1182 1182 Phosphotyrosine.  
Keyword
 Complete proteome; mRNA processing; mRNA splicing; Nucleus; Phosphoprotein; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1330 AA 
Protein Sequence
MGESDAESDS SSNSSSSDTS SGSDSDARSE SSSSESSGRE EEEAKQEESA KDAKKTDDTD 60
RGEKRAKERD AGQDEQPTEQ KKTPAAEPRS ERQHISHSAG VEKQQEEAVA AAEAESEKLN 120
EAKKVETPVQ RKEEAEASSV TKELNSPKAQ EENAARELEE RRKDEEQPVT TNGSSKESPV 180
EAAAETVKPP TADHIEEGEI TDKDEDDLPT KEEKKAVASK SPPKETQRKQ SRSPDGKRRR 240
PRSSSRSPSP SSRRRRRSRS KGSRTRSRSK SPIRRRSNSL ERRRVERQRR HEERDKRDEE 300
RAKEREKRHQ KGEPTSSRRR DDSREKKRSP ERKRDRSSST PKSKSSKTPR HTETTETNAD 360
NETVTEPAAK ITERQRKTVD VLTSRTGGAY IPPAKLRMMQ SQITDKSSAA YQRIAWEALK 420
KSIHGYINKV NVTNIAIITR ELLRENIVRG RGLLSRSIIQ AQAASPTFTH VYAALVSIIN 480
SKFPNIGELL LKRLVIQFRR AFRRNDKMVC MSATRFIGHL VNQRVAHEIL ALEILTLLVE 540
TPTDDSVEVA IAFLKECGMK LTEVSSKGIG AIFEMLRNIL HEGKLDKRVQ YMIEVLFQIR 600
KDGFKDHQAV VPELELVEED DQFTHLMMLD EATETEDILN VFKFDDNYAE NEDKYKGLSR 660
EILGSDDGSS SGSGSGSDSD SDSDGESGSD AEKKAEAGDI IDSTETNLIA LRRTIYLTIN 720
SSLDYEECAH KLMKMQLKPG QEIELCHMFL DCCAEQRTYE KFYGLLAQRF CNINKIYIPP 780
FEEIFKDTYQ TTHRLDTNRL RNVSKFFAHL LFTDAISWDV LECIQLNEDD TTSSSRIFIK 840
ILFQELAEYM GLGKLNAKLK DDVLVESIAG LFPKDNPRNT RFSINFFTSI GLGGLTDDLR 900
RFLKNAPKSV PAINAEILAN AGGNPFRDGS APAGNTKVAP SSSSSSSSSS DTDSEDSSEE 960
DSSSDSSSES SSSDSSSEPK KKRKRKDKDK KKSKKATKEK SKKTKNKKKK KKAEKEQEKE 1020
KEKQRKSKKE KEKDKKRKKE EKKAAKKKSK HRRKSQESSD SSGSEDSDKS TSESSDSSNS 1080
SSDESDAEPQ AKIKRQEHVE KNKFRGRTQD SDEFNLEGPG SKNRFQPNGN GQRRRDNSTG 1140
RERNRENSSY DRERNRGNSS YDRERKRGNS SYDRERNRES SYDKERKNRN AVAHDRQRKR 1200
DRSRSYERPT IRENSAPREK RMESSRSEKD SRRGDRSSRN ERSDRGERSD RGERSDRGER 1260
SDRGERSDRG ERSDRGERSD REKERSRAKE RERDRDRDLK GQRERKRERD DGSRDRSRRE 1320
RSSRRSKGRS 1330 
Gene Ontology
 GO:0005681; C:spliceosomal complex; ISS:UniProtKB.
 GO:0003677; F:DNA binding; IEA:InterPro.
 GO:0003723; F:RNA binding; ISS:UniProtKB.
 GO:0006397; P:mRNA processing; IEA:UniProtKB-KW.
 GO:0006911; P:phagocytosis, engulfment; IMP:FlyBase.
 GO:0008380; P:RNA splicing; IEA:UniProtKB-KW. 
Interpro
 IPR016024; ARM-type_fold.
 IPR003891; Initiation_fac_eIF4g_MI.
 IPR016021; MIF4-like_typ_1/2/3.
 IPR003890; MIF4G-like_typ-3. 
Pfam
 PF02847; MA3
 PF02854; MIF4G 
SMART
 SM00544; MA3
 SM00543; MIF4G 
PROSITE
 PS51366; MI 
PRINTS