| Tag | Content |
|---|
| CPLM ID | CPLM-001433 |
UniProt Accession | |
Genbank Protein ID | |
Genbank Nucleotide ID | |
Protein Name | 7-alpha-hydroxycholest-4-en-3-one 12-alpha-hydroxylase |
Protein Synonyms/Alias | 7-alpha-hydroxy-4-cholesten-3-one 12-alpha-hydroxylase; CYPVIIIB1; Cytochrome P450 8B1; Sterol 12-alpha-hydroxylase |
Gene Name | Cyp8b1 |
Gene Synonyms/Alias | Cyp12 |
Created Date | July 27, 2013 |
Organism | Mus musculus (Mouse) |
NCBI Taxa ID | 10090 |
Lysine Modification | Position | Peptide | Type | References |
|---|
| 50 | GHSMAFRKNMFEFLK | ubiquitination | [1] | | 57 | KNMFEFLKGMRAKHG | ubiquitination | [1] | | 94 | PIIKNTEKALDFQSY | ubiquitination | [1] | | 103 | LDFQSYAKELVLKVF | ubiquitination | [1] | | 108 | YAKELVLKVFGYQSV | ubiquitination | [1] | | 189 | LSLFGYTKDKQQDLD | ubiquitination | [1] | | 191 | LFGYTKDKQQDLDEA | ubiquitination | [1] | | 323 | GKARLEAKKSFTFTP | ubiquitination | [1] | | 324 | KARLEAKKSFTFTPS | ubiquitination | [1] | | 334 | TFTPSALKHTPVLDS | ubiquitination | [1] | | 366 | VQEDYVLKMASGQEY | ubiquitination | [1] | | 380 | YQIRRGDKVALFPYL | ubiquitination | [1] | | 403 | HPEPTAFKYDRFLNP | ubiquitination | [1] | | 420 | TRKVDFYKSGKKIHH | ubiquitination | [1] | | 424 | DFYKSGKKIHHYSMP | ubiquitination | [1] | | 438 | PWGSGVSKCPGRFFA | ubiquitination | [1] |
|
Reference | [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues. Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C. Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [ PMID: 22790023] |
Functional Description | Involved in bile acid synthesis and is responsible for the conversion of 7 alpha-hydroxy-4-cholesten-3-one into 7 alpha, 12 alpha-dihydroxy-4-cholesten-3-one. Responsible for the balance between formation of cholic acid and chenodeoxycholic acid. Has a rather broad substrate specificity including a number of 7-alpha- hydroxylated C27 steroids. |
Sequence Annotation | METAL 439 439 Iron (heme axial ligand) (By similarity). |
Keyword | Complete proteome; Endoplasmic reticulum; Heme; Iron; Membrane; Metal-binding; Microsome; Monooxygenase; NADP; Oxidoreductase; Polymorphism; Reference proteome; Transmembrane; Transmembrane helix. |
Sequence Source | UniProt (SWISSPROT/TrEMBL); GenBank; EMBL |
Protein Length | 500 AA |
Protein Sequence | MTLWCTVLGA LLTVVGCLCL SLLLRHRRPW EPPLDKGFVP WLGHSMAFRK NMFEFLKGMR 60
AKHGDVFTVQ LGGQYFTFVM DPLSFGPIIK NTEKALDFQS YAKELVLKVF GYQSVDGDHR 120
MIHLASTKHL MGQGLEELNQ AMLDSLSLVM LGPKGSSLGA SSWCEDGLFH FCYRILFKAG 180
FLSLFGYTKD KQQDLDEADE LFRKFRRFDF LFPRFVYSLL GPREWVEVSQ LQRLFHQRLS 240
VEQNLEKDGI SCWLGYMLQF LREQGIASSM QDKFNFMMLW ASQGNTGPTC FWVLLFLLKH 300
QDAMKAVREE ATRVMGKARL EAKKSFTFTP SALKHTPVLD SVMEESLRLC ATPTLLRVVQ 360
EDYVLKMASG QEYQIRRGDK VALFPYLSVH MDPDIHPEPT AFKYDRFLNP DGTRKVDFYK 420
SGKKIHHYSM PWGSGVSKCP GRFFALSEMK TFVLLMIMYF DFKLVDPDIP VPPIDPRRWG 480
FGTSQPSHEV RFLYRLKPVQ 500 |
Gene Ontology | GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell. GO:0016021; C:integral to membrane; IEA:UniProtKB-KW. GO:0033778; F:7alpha-hydroxycholest-4-en-3-one 12alpha-hydroxylase activity; IEA:EC. GO:0009055; F:electron carrier activity; IEA:InterPro. GO:0020037; F:heme binding; IEA:InterPro. GO:0005506; F:iron ion binding; IEA:InterPro. |
Interpro | |
Pfam | |
SMART | |
PROSITE | |
PRINTS | |