UniProt Accession

 MCES_HUMAN; O43148; B0YJ90; D3DUJ5; O94996; Q9UIJ9 

Genbank Protein ID

 AB022604; AB022605; AF067791; AB020966; AB007858; EF445026; CH471113; CH471113; BC036798 

Genbank Nucleotide ID

 BAA74464.1; BAA74463.1; AAC63269.1; BAA82447.1; BAA23694.1; ACA06068.1; EAX01505.1; EAX01506.1; AAH36798.1 

Protein Name

 mRNA cap guanine-N7 methyltransferase 

Protein Synonyms/Alias

 RG7MT1; mRNA (guanine-N(7)-)-methyltransferase; mRNA cap methyltransferase; hCMT1; hMet; hcm1p 

Gene Name

 RNMT 

Gene Synonyms/Alias

 KIAA0398 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
126	TGDGTQNKRKIALED	acetylation	[1]
128	DGTQNKRKIALEDVP	acetylation	[1]
137	ALEDVPEKQKNLEEG	acetylation	[1]
163	LQEVGLEKRSQSRIF	ubiquitination	[2, 3]
428	YPANESSKLVSEKVD	ubiquitination	[2]
433	SSKLVSEKVDDYEHA	ubiquitination	[2]

Reference

 [1] Regulation of cellular metabolism by protein lysine acetylation.
 Zhao S, Xu W, Jiang W, Yu W, Lin Y, Zhang T, Yao J, Zhou L, Zeng Y, Li H, Li Y, Shi J, An W, Hancock SM, He F, Qin L, Chin J, Yang P, Chen X, Lei Q, Xiong Y, Guan KL.
 Science. 2010 Feb 19;327(5968):1000-4. [PMID: 20167786]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961] 

Functional Description

 mRNA-capping methyltransferase that methylates the N7 position of the added guanosine to the 5'-cap structure of mRNAs. Binds RNA containing 5'-terminal GpppC. 

Sequence Annotation

 DOMAIN 128 476 mRNA cap 0 methyltransferase.
 REGION 176 177 mRNA cap binding (By similarity).
 REGION 261 262 S-adenosyl-L-methionine binding (By
 REGION 284 286 S-adenosyl-L-methionine binding (By
 MOTIF 126 128 Nuclear localization signal.
 BINDING 180 180 S-adenosyl-L-methionine (By similarity).
 BINDING 205 205 S-adenosyl-L-methionine; via carbonyl
 BINDING 227 227 S-adenosyl-L-methionine (By similarity).
 BINDING 288 288 mRNA cap (By similarity).
 BINDING 370 370 mRNA cap (By similarity).
 BINDING 467 467 mRNA cap (By similarity).
 MOD_RES 24 24 Phosphoserine (By similarity).
 MOD_RES 28 28 Phosphoserine (By similarity).  

Keyword

 3D-structure; Alternative splicing; Complete proteome; Methyltransferase; mRNA capping; mRNA processing; Nucleus; Phosphoprotein; Reference proteome; RNA-binding; S-adenosyl-L-methionine; Transferase. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 476 AA 

Protein Sequence

Gene Ontology

 GO:0005845; C:mRNA cap binding complex; IDA:UniProtKB.
 GO:0005730; C:nucleolus; IDA:HPA.
 GO:0005654; C:nucleoplasm; TAS:Reactome.
 GO:0004482; F:mRNA (guanine-N7-)-methyltransferase activity; IDA:UniProtKB.
 GO:0003723; F:RNA binding; IMP:UniProtKB.
 GO:0006366; P:transcription from RNA polymerase II promoter; TAS:Reactome.
 GO:0022415; P:viral reproductive process; TAS:Reactome. 

Interpro

 IPR016899; mRNA_G-N7_MeTrfase.
 IPR004971; mRNA_G-N7_MeTrfase_dom. 

Pfam

 PF03291; Pox_MCEL 

SMART

  

PROSITE

 PS51562; RNA_CAP0_MT 

PRINTS