CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-000295
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Transcription elongation regulator 1 
Protein Synonyms/Alias
 TATA box-binding protein-associated factor 2S; Transcription factor CA150 
Gene Name
 TCERG1 
Gene Synonyms/Alias
 CA150; TAF2S 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
444EYKTADGKTYYYNNRubiquitination[1]
503EEPIKEIKEEPKEEEsumoylation[2]
522EKAAQKAKPVATAPIubiquitination[3]
579IQEPPHKKGMEELKKubiquitination[3]
585KKGMEELKKLRHPTPubiquitination[3]
586KGMEELKKLRHPTPTubiquitination[3]
608QFSMSAIKEEQELMEsumoylation[2]
624INEDEPVKAKKRKRDubiquitination[4]
640NKDIDSEKEAAMEAEubiquitination[4]
683SAFSTWEKELHKIVFubiquitination[3, 5, 6]
687TWEKELHKIVFDPRYubiquitination[3]
700RYLLLNPKERKQVFDubiquitination[3]
711QVFDQYVKTRAEEERubiquitination[3]
740KKMMEEAKFNPRATFubiquitination[3]
761HAKDSRFKAIEKMKDacetylation[7]
944LEREEKEKLFNEHIEubiquitination[5, 6]
992KEDPRCIKFSSSDRKubiquitination[1, 3]
Reference
 [1] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [2] Differential effects of sumoylation on transcription and alternative splicing by transcription elongation regulator 1 (TCERG1).
 Sánchez-Alvarez M, Montes M, Sánchez-Hernández N, Hernández-Munain C, Suñé C.
 J Biol Chem. 2010 May 14;285(20):15220-33. [PMID: 20215116]
 [3] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [4] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [5] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [6] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [7] Regulation of cellular metabolism by protein lysine acetylation.
 Zhao S, Xu W, Jiang W, Yu W, Lin Y, Zhang T, Yao J, Zhou L, Zeng Y, Li H, Li Y, Shi J, An W, Hancock SM, He F, Qin L, Chin J, Yang P, Chen X, Lei Q, Xiong Y, Guan KL.
 Science. 2010 Feb 19;327(5968):1000-4. [PMID: 20167786] 
Functional Description
 Transcription factor that binds RNA polymerase II and inhibits the elongation of transcripts from target promoters. Regulates transcription elongation in a TATA box-dependent manner. Necessary for TAT-dependent activation of the human immunodeficiency virus type 1 (HIV-1) promoter. 
Sequence Annotation
 DOMAIN 131 164 WW 1.
 DOMAIN 429 462 WW 2.
 DOMAIN 528 561 WW 3.
 DOMAIN 659 712 FF 1.
 DOMAIN 725 779 FF 2.
 DOMAIN 791 846 FF 3.
 DOMAIN 896 952 FF 4.
 DOMAIN 954 1010 FF 5.
 DOMAIN 1012 1077 FF 6.
 MOTIF 626 630 Nuclear localization signal (Potential).  
Keyword
 3D-structure; Activator; Alternative splicing; Coiled coil; Complete proteome; Direct protein sequencing; Nucleus; Reference proteome; Repeat; Repressor; Transcription; Transcription regulation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1098 AA 
Protein Sequence
MAERGGDGGE SERFNPGELR MAQQQALRFR GPAPPPNAVM RGPPPLMRPP PPFGMMRGPP 60
PPPRPPFGRP PFDPNMPPMP PPGGIPPPMG PPHLQRPPFM PPPMSSMPPP PGMMFPPGMP 120
PVTAPGTPAL PPTEEIWVEN KTPDGKVYYY NARTRESAWT KPDGVKVIQQ SELTPMLAAQ 180
AQVQAQAQAQ AQAQAQAQAQ AQAQAQAQAQ AQAQAQAQAQ AQAQAQAQAQ AQAQAQAQAQ 240
AQVQAQVQAQ VQAQAVGAST PTTSSPAPAV STSTSSSTPS STTSTTTTAT SVAQTVSTPT 300
TQDQTPSSAV SVATPTVSVS TPAPTATPVQ TVPQPHPQTL PPAVPHSVPQ PTTAIPAFPP 360
VMVPPFRVPL PGMPIPLPGV AMMQIVSCPY VKTVATTKTG VLPGMAPPIV PMIHPQVAIA 420
ASPATLAGAT AVSEWTEYKT ADGKTYYYNN RTLESTWEKP QELKEKEKLE EKIKEPIKEP 480
SEEPLPMETE EEDPKEEPIK EIKEEPKEEE MTEEEKAAQK AKPVATAPIP GTPWCVVWTG 540
DERVFFYNPT TRLSMWDRPD DLIGRADVDK IIQEPPHKKG MEELKKLRHP TPTMLSIQKW 600
QFSMSAIKEE QELMEEINED EPVKAKKRKR DDNKDIDSEK EAAMEAEIKA ARERAIVPLE 660
ARMKQFKDML LERGVSAFST WEKELHKIVF DPRYLLLNPK ERKQVFDQYV KTRAEEERRE 720
KKNKIMQAKE DFKKMMEEAK FNPRATFSEF AAKHAKDSRF KAIEKMKDRE ALFNEFVAAA 780
RKKEKEDSKT RGEKIKSDFF ELLSNHHLDS QSRWSKVKDK VESDPRYKAV DSSSMREDLF 840
KQYIEKIAKN LDSEKEKELE RQARIEASLR EREREVQKAR SEQTKEIDRE REQHKREEAI 900
QNFKALLSDM VRSSDVSWSD TRRTLRKDHR WESGSLLERE EKEKLFNEHI EALTKKKREH 960
FRQLLDETSA ITLTSTWKEV KKIIKEDPRC IKFSSSDRKK QREFEEYIRD KYITAKADFR 1020
TLLKETKFIT YRSKKLIQES DQHLKDVEKI LQNDKRYLVL DCVPEERRKL IVAYVDDLDR 1080
RGPPPPPTAS EPTRRSTK 1098 
Gene Ontology
 GO:0005634; C:nucleus; TAS:ProtInc.
 GO:0001106; F:RNA polymerase II transcription corepressor activity; IDA:BHF-UCL.
 GO:0003713; F:transcription coactivator activity; TAS:ProtInc. 
Interpro
 IPR002713; FF_domain.
 IPR001202; WW_dom. 
Pfam
 PF01846; FF
 PF00397; WW 
SMART
 SM00441; FF
 SM00456; WW 
PROSITE
 PS51676; FF
 PS01159; WW_DOMAIN_1
 PS50020; WW_DOMAIN_2 
PRINTS