CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-020415
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Probable E3 ubiquitin-protein ligase TRIM8 
Protein Synonyms/Alias
 Glioblastoma-expressed RING finger protein; RING finger protein 27; Tripartite motif-containing protein 8 
Gene Name
 TRIM8 
Gene Synonyms/Alias
 GERP; RNF27 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
45CIGEAWAKDSGLVRCubiquitination[1]
70PGLEKNLKLTNIVEKubiquitination[1]
195EIRKMLMKQQDRLEEubiquitination[1]
213DIEDQLYKLESDKRLubiquitination[1]
238EVRLQYEKLHQLLDEubiquitination[1]
256QTVEVLDKAQAKFCSubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961] 
Functional Description
 Probable E3 ubiquitin-protein ligase which may promote proteasomal degradation of SOCS1 (By similarity). 
Sequence Annotation
 ZN_FING 15 56 RING-type.
 ZN_FING 92 132 B box-type 1.
 ZN_FING 140 182 B box-type 2.  
Keyword
 Coiled coil; Complete proteome; Ligase; Metal-binding; Reference proteome; Repeat; Ubl conjugation pathway; Zinc; Zinc-finger. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 551 AA 
Protein Sequence
MAENWKNCFE EELICPICLH VFVEPVQLPC KHNFCRGCIG EAWAKDSGLV RCPECNQAYN 60
QKPGLEKNLK LTNIVEKFNA LHVEKPPAAL HCVFCRRGPP LPAQKVCLRC EAPCCQSHVQ 120
THLQQPSTAR GHLLVEADDV RAWSCPQHNA YRLYHCEAEQ VAVCQYCCYY SGAHQGHSVC 180
DVEIRRNEIR KMLMKQQDRL EEREQDIEDQ LYKLESDKRL VEEKVNQLKE EVRLQYEKLH 240
QLLDEDLRQT VEVLDKAQAK FCSENAAQAL HLGERMQEAK KLLGSLQLLF DKTEDVSFMK 300
NTKSVKILMD RTQTCTSSSL SPTKIGHLNS KLFLNEVAKK EKQLRKMLEG PFSTPVPFLQ 360
SVPLYPCGVS SSGAEKRKHS TAFPEASFLE TSSGPVGGQY GAAGTASGEG QSGQPLGPCS 420
STQHLVALPG GAQPVHSSPV FPPSQYPNGS AAQQPMLPQY GGRKILVCSV DNCYCSSVAN 480
HGGHQPYPRS GHFPWTVPSQ EYSHPLPPTP SVPQSLPSLA VRDWLDASQQ PGHQDFYRVY 540
GQPSTKHYVT S 551 
Gene Ontology
 GO:0005737; C:cytoplasm; IDA:HPA.
 GO:0016605; C:PML body; IDA:UniProtKB.
 GO:0016874; F:ligase activity; IEA:UniProtKB-KW.
 GO:0008270; F:zinc ion binding; NAS:UniProtKB.
 GO:0016567; P:protein ubiquitination; IEA:UniProtKB-UniPathway. 
Interpro
 IPR018957; Znf_C3HC4_RING-type.
 IPR001841; Znf_RING.
 IPR013083; Znf_RING/FYVE/PHD.
 IPR017907; Znf_RING_CS. 
Pfam
 PF00097; zf-C3HC4 
SMART
 SM00184; RING 
PROSITE
 PS50119; ZF_BBOX
 PS00518; ZF_RING_1
 PS50089; ZF_RING_2 
PRINTS