| Tag | Content |
|---|
| CPLM ID | CPLM-018122 |
UniProt Accession | |
Genbank Protein ID | |
Genbank Nucleotide ID | |
Protein Name | Mediator of RNA polymerase II transcription subunit 25 |
Protein Synonyms/Alias | Mediator complex subunit 25; mMED25 |
Gene Name | Med25 |
Gene Synonyms/Alias | |
Created Date | July 27, 2013 |
Organism | Mus musculus (Mouse) |
NCBI Taxa ID | 10090 |
Lysine Modification | Position | Peptide | Type | References |
|---|
| 197 | ALRLLFEKAAPPALL | ubiquitination | [1] | | 484 | NKDLESLKGLYRIMG | ubiquitination | [1] |
|
Reference | [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues. Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C. Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [ PMID: 22790023] |
Functional Description | Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Required for RARA/RXRA-mediated transcription (By similarity). |
Sequence Annotation | REGION 1 226 Interaction with the Mediator complex (By
REGION 389 543 Interaction with VP16 (By similarity).
REGION 395 545 Interaction with CREBBP (By similarity).
REGION 563 652 Interaction with RARA (By similarity).
REGION 639 705 Interaction with RARA (By similarity).
MOTIF 645 649 LXXLL motif. |
Keyword | Activator; Alternative splicing; Complete proteome; Nucleus; Reference proteome; Transcription; Transcription regulation. |
Sequence Source | UniProt (SWISSPROT/TrEMBL); GenBank; EMBL |
Protein Length | 745 AA |
Protein Sequence | MVPGSEGPAR AGGLVADVVF VIEGTANLGP YFEELRKHYL LPAIEYFNGG PPAETDFGGD 60
YGGTQYSLVV FNTVDCAPES YVQCHAPTSS AYEFVTWLDG IKFMGGGGES CSLIAEGLST 120
ALQLFDDFKK MREQIGQTHR VCLLICNSPP YLLPAVESTT YSGCTTESLV QKIGERGIHF 180
SIVSPRKLPA LRLLFEKAAP PALLEPLQQP ADVSQDPRHM VLVRGLVLPV GGSSTSGSLQ 240
TKQAVPLPPA PASAATLSAA PPQALPPVPP QYQVPGNLSA AQVAAQNAVE AAKSQKAGLG 300
PRFSPINPLQ QAAPGVGPPF SQAPAPPLAP VPPGAPKPPP ASQPSLVSTV APGPVLAAPA 360
QPGAPSLAGT VTPGGVNGPS AAQLGGPALG GQQSVSNKLL AWSGVLEWQE KPKPASVDAN 420
TKLTRSLPCQ VYVNHGENLK TEQWPQKLIM QLIPQQLLTT LGPLFRNSRM VQFHFTNKDL 480
ESLKGLYRIM GNGFAGCVHF PHTAPCEVRV LMLLYSSKKK IFMGLIPYDQ SGFVNGIRQV 540
ITNHKQVQQQ KLEQQRGMGA QQAPPVLGPI LEEQARPPQN LLQLRAPQPQ PQGAVGASAA 600
TGQPQPQGAT QAPTGAPQGP PGAAPGPPPS GPILRPQNPG ANPQLRSLLL NPAPPQTGVP 660
PPQASLHHLQ PPGAPTLLPP HQSMGQPQLG PQLLHPPPAQ SWPTQLPQRA PLPGQMLLSG 720
GPRGPVPQPG LQPSVMEDDI LMDLI 745 |
Gene Ontology | GO:0005654; C:nucleoplasm; TAS:Reactome. GO:0048147; P:negative regulation of fibroblast proliferation; IEA:Compara. GO:0000122; P:negative regulation of transcription from RNA polymerase II promoter; IEA:Compara. GO:0071158; P:positive regulation of cell cycle arrest; IEA:Compara. GO:0035563; P:positive regulation of chromatin binding; IEA:Compara. GO:2001178; P:positive regulation of mediator complex assembly; IEA:Compara. GO:0045944; P:positive regulation of transcription from RNA polymerase II promoter; IEA:Compara. GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW. |
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