CPLM-020905

Genbank Nucleotide ID

m7GpppX diphosphatase

Protein Synonyms/Alias

DCS-1; Decapping scavenger enzyme; Hint-related 7meGMP-directed hydrolase; Histidine triad nucleotide-binding protein 5; Histidine triad protein member 5; HINT-5; Scavenger mRNA-decapping enzyme DcpS

Mus musculus (Mouse)

Position	Peptide	Type	References
127	PRHLSDIKTTVVYPA	ubiquitination	[1]
137	VVYPATEKHLQKYMR	acetylation	[2]
137	VVYPATEKHLQKYMR	ubiquitination	[1]
332	PLLQLLQKAQQERN*	ubiquitination	[1]

[1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
[2] Calorie restriction and SIRT3 trigger global reprogramming of the mitochondrial protein acetylome.
Hebert AS, Dittenhafer-Reed KE, Yu W, Bailey DJ, Selen ES, Boersma MD, Carson JJ, Tonelli M, Balloon AJ, Higbee AJ, Westphall MS, Pagliarini DJ, Prolla TA, Assadi-Porter F, Roy S, Denu JM, Coon JJ.
Mol Cell. 2013 Jan 10;49(1):186-99. [PMID: 23201123]

Functional Description

Decapping scavenger enzyme that catalyzes the cleavage of a residual cap structure following the degradation of mRNAs by the 3'->5' exosome-mediated mRNA decay pathway. Hydrolyzes cap analog structures like 7-methylguanosine nucleoside triphosphate (m7GpppG) with up to 10 nucleotide substrates (small capped oligoribonucleotides) and specifically releases 5'-phosphorylated RNA fragments and 7-methylguanosine monophosphate (m7GMP). Cleaves cap analog structures like tri-methyl guanosine nucleoside triphosphate (m3(2,2,7)GpppG) with very poor efficiency. Does not hydrolyze unmethylated cap analog (GpppG) and shows no decapping activity on intact m7GpppG-capped mRNA molecules longer than 25 nucleotides. Does not hydrolyze 7-methylguanosine diphosphate (m7GDP) to m7GMP. May also play a role in the 5'->3 mRNA decay pathway; m7GDP, the downstream product released by the 5'->3' mRNA mediated decapping activity, may be also converted by DCPS to m7GMP. Binds to m7GpppG and strongly to m7GDP. Plays a role in first intron splicing of pre-mRNAs. Inhibits activation-induced cell death (By similarity).

REGION 267 278 Substrate binding (By similarity).
MOTIF 9 12 nuclear localization signal (NLS) (By
MOTIF 141 153 nuclear export sequence (NES) (By
MOTIF 274 278 Histidine triad motif (By similarity).
ACT_SITE 276 276 Nucleophile (By similarity).
BINDING 174 174 Substrate (By similarity).
BINDING 184 184 Substrate (By similarity).
BINDING 204 204 Substrate (By similarity).
BINDING 206 206 Substrate (By similarity).
MOD_RES 2 2 N-acetylalanine (By similarity).
MOD_RES 21 21 Phosphothreonine.
MOD_RES 137 137 N6-acetyllysine (By similarity).
MOD_RES 141 141 N6-acetyllysine (By similarity).

3D-structure; Acetylation; Complete proteome; Cytoplasm; Hydrolase; mRNA processing; mRNA splicing; Nucleus; Phosphoprotein; Reference proteome.

UniProt (SWISSPROT/TrEMBL); GenBank; EMBL

GO:0005737; C:cytoplasm; ISS:UniProtKB.
GO:0005739; C:mitochondrion; IEA:Compara.
GO:0005634; C:nucleus; ISS:UniProtKB.
GO:0050072; F:m7G(5')pppN diphosphatase activity; ISS:UniProtKB.
GO:0000340; F:RNA 7-methylguanosine cap binding; ISS:UniProtKB.
GO:0036245; P:cellular response to menadione; ISS:UniProtKB.
GO:0000290; P:deadenylation-dependent decapping of nuclear-transcribed mRNA; IEA:InterPro.
GO:0045292; P:mRNA cis splicing, via spliceosome; ISS:UniProtKB.
GO:0043069; P:negative regulation of programmed cell death; ISS:UniProtKB.

IPR008594; DcpS/DCS2.
IPR019808; Histidine_triad_CS.
IPR011146; HIT-like.
IPR011145; Scavenger_mRNA_decap_enz_N.