CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-008730
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Clathrin heavy chain 2 
Protein Synonyms/Alias
 Clathrin heavy chain on chromosome 22; CLH-22 
Gene Name
 CLTCL1 
Gene Synonyms/Alias
 CLH22; CLTCL; CLTD 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
83ASKVIALKAGKTLQIubiquitination[1]
86VIALKAGKTLQIFNIubiquitination[1, 2]
96QIFNIEMKSKMKAHTubiquitination[1, 2]
98FNIEMKSKMKAHTMAubiquitination[1]
189QLYSVDRKVSQPIEGubiquitination[3]
450GRKQLLEKWLKEDKLacetylation[4]
453QLLEKWLKEDKLECSubiquitination[1, 2]
506QKIVLYAKKVGYTPDacetylation[4]
506QKIVLYAKKVGYTPDubiquitination[1]
507KIVLYAKKVGYTPDWubiquitination[1, 2, 5, 6]
637YTDLYDIKRAVVHTHubiquitination[2]
737KYIQAACKTGQIKEVubiquitination[1]
742ACKTGQIKEVERICRubiquitination[1, 2]
760CYNPERVKNFLKEAKubiquitination[1]
764ERVKNFLKEAKLTDQacetylation[4]
806YIEIYVQKVNPSRTPubiquitination[2, 6]
851ELVAEVEKRNRLKLLubiquitination[1]
911VVGRYCEKRDPHLACubiquitination[1, 6]
941CNENSLFKSEARYLVubiquitination[1]
1034LLILTAIKADRTRVMubiquitination[1, 2, 5, 6]
1254ASSTRTWKEVCFACMubiquitination[7]
1347WSRVNIPKVLRAAEQubiquitination[2]
1392AWKEGQFKDIITKVAubiquitination[2]
1397QFKDIITKVANVELCubiquitination[7]
1449AGQLPLVKPYLRSVQacetylation[4]
1449AGQLPLVKPYLRSVQubiquitination[1, 2, 7]
1562QWFLEEGKRECFAACubiquitination[7]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [4] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
 [5] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [6] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [7] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094
Functional Description
 Clathrin is the major protein of the polyhedral coat of coated pits and vesicles. Two different adapter protein complexes link the clathrin lattice either to the plasma membrane or to the trans-Golgi network (By similarity). 
Sequence Annotation
 REPEAT 537 683 CHCR 1.
 REPEAT 686 828 CHCR 2.
 REPEAT 833 972 CHCR 3.
 REPEAT 979 1124 CHCR 4.
 REPEAT 1128 1269 CHCR 5.
 REPEAT 1274 1420 CHCR 6.
 REPEAT 1423 1566 CHCR 7.
 REGION 1 479 Globular terminal domain.
 REGION 24 67 WD40-like repeat 1.
 REGION 68 107 WD40-like repeat 2.
 REGION 108 149 WD40-like repeat 3.
 REGION 150 195 WD40-like repeat 4.
 REGION 196 257 WD40-like repeat 5.
 REGION 258 301 WD40-like repeat 6.
 REGION 302 330 WD40-like repeat 7.
 REGION 449 465 Binding site for the uncoating ATPase,
 REGION 480 523 Flexible linker.
 REGION 524 1640 Heavy chain arm.
 REGION 524 634 Distal segment.
 REGION 639 1640 Proximal segment.
 REGION 1213 1522 Involved in binding clathrin light chain
 REGION 1551 1640 Trimerization (By similarity).  
Keyword
 Alternative splicing; Coated pit; Complete proteome; Cytoplasmic vesicle; Membrane; Polymorphism; Reference proteome; Repeat. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1640 AA 
Protein Sequence
MAQILPVRFQ EHFQLQNLGI NPANIGFSTL TMESDKFICI REKVGEQAQV TIIDMSDPMA 60
PIRRPISAES AIMNPASKVI ALKAGKTLQI FNIEMKSKMK AHTMAEEVIF WKWVSVNTVA 120
LVTETAVYHW SMEGDSQPMK MFDRHTSLVG CQVIHYRTDE YQKWLLLVGI SAQQNRVVGA 180
MQLYSVDRKV SQPIEGHAAA FAEFKMEGNA KPATLFCFAV RNPTGGKLHI IEVGQPAAGN 240
QPFVKKAVDV FFPPEAQNDF PVAMQIGAKH GVIYLITKYG YLHLYDLESG VCICMNRISA 300
DTIFVTAPHK PTSGIIGVNK KGQVLSVCVE EDNIVNYATN VLQNPDLGLR LAVRSNLAGA 360
EKLFVRKFNT LFAQGSYAEA AKVAASAPKG ILRTRETVQK FQSIPAQSGQ ASPLLQYFGI 420
LLDQGQLNKL ESLELCHLVL QQGRKQLLEK WLKEDKLECS EELGDLVKTT DPMLALSVYL 480
RANVPSKVIQ CFAETGQFQK IVLYAKKVGY TPDWIFLLRG VMKISPEQGL QFSRMLVQDE 540
EPLANISQIV DIFMENSLIQ QCTSFLLDAL KNNRPAEGLL QTWLLEMNLV HAPQVADAIL 600
GNKMFTHYDR AHIAQLCEKA GLLQQALEHY TDLYDIKRAV VHTHLLNPEW LVNFFGSLSV 660
EDSVECLHAM LSANIRQNLQ LCVQVASKYH EQLGTQALVE LFESFKSYKG LFYFLGSIVN 720
FSQDPDVHLK YIQAACKTGQ IKEVERICRE SSCYNPERVK NFLKEAKLTD QLPLIIVCDR 780
FGFVHDLVLY LYRNNLQRYI EIYVQKVNPS RTPAVIGGLL DVDCSEEVIK HLIMAVRGQF 840
STDELVAEVE KRNRLKLLLP WLESQIQEGC EEPATHNALA KIYIDSNNSP ECFLRENAYY 900
DSSVVGRYCE KRDPHLACVA YERGQCDLEL IKVCNENSLF KSEARYLVCR KDPELWAHVL 960
EETNPSRRQL IDQVVQTALS ETRDPEEISV TVKAFMTADL PNELIELLEK IVLDNSVFSE 1020
HRNLQNLLIL TAIKADRTRV MEYISRLDNY DALDIASIAV SSALYEEAFT VFHKFDMNAS 1080
AIQVLIEHIG NLDRAYEFAE RCNEPAVWSQ LAQAQLQKDL VKEAINSYIR GDDPSSYLEV 1140
VQSASRSNNW EDLVKFLQMA RKKGRESYIE TELIFALAKT SRVSELEDFI NGPNNAHIQQ 1200
VGDRCYEEGM YEAAKLLYSN VSNFARLAST LVHLGEYQAA VDNSRKASST RTWKEVCFAC 1260
MDGQEFRFAQ LCGLHIVIHA DELEELMCYY QDRGYFEELI LLLEAALGLE RAHMGMFTEL 1320
AILYSKFKPQ KMLEHLELFW SRVNIPKVLR AAEQAHLWAE LVFLYDKYEE YDNAVLTMMS 1380
HPTEAWKEGQ FKDIITKVAN VELCYRALQF YLDYKPLLIN DLLLVLSPRL DHTWTVSFFS 1440
KAGQLPLVKP YLRSVQSHNN KSVNEALNHL LTEEEDYQGL RASIDAYDNF DNISLAQQLE 1500
KHQLMEFRCI AAYLYKGNNW WAQSVELCKK DHLYKDAMQH AAESRDAELA QKLLQWFLEE 1560
GKRECFAACL FTCYDLLRPD MVLELAWRHN LVDLAMPYFI QVMREYLSKV DKLDALESLR 1620
KQEEHVTEPA PLVFDFDGHE 1640 
Gene Ontology
 GO:0030132; C:clathrin coat of coated pit; IEA:InterPro.
 GO:0030130; C:clathrin coat of trans-Golgi network vesicle; IEA:InterPro.
 GO:0030136; C:clathrin-coated vesicle; IDA:UniProtKB.
 GO:0005905; C:coated pit; IDA:UniProtKB.
 GO:0005819; C:spindle; IDA:UniProtKB.
 GO:0005802; C:trans-Golgi network; IDA:UniProtKB.
 GO:0004871; F:signal transducer activity; TAS:ProtInc.
 GO:0005198; F:structural molecule activity; IEA:InterPro.
 GO:0009653; P:anatomical structure morphogenesis; TAS:ProtInc.
 GO:0006886; P:intracellular protein transport; IEA:InterPro.
 GO:0007067; P:mitosis; IDA:UniProtKB.
 GO:0046326; P:positive regulation of glucose import; IMP:UniProtKB.
 GO:0006898; P:receptor-mediated endocytosis; IDA:UniProtKB. 
Interpro
 IPR016024; ARM-type_fold.
 IPR000547; Clathrin_H-chain/VPS_repeat.
 IPR016025; Clathrin_H-chain_link/propller.
 IPR015348; Clathrin_H-chain_linker_core.
 IPR001473; Clathrin_H-chain_propeller_N.
 IPR022365; Clathrin_H-chain_propeller_rpt.
 IPR016341; Clathrin_heavy_chain.
 IPR011990; TPR-like_helical. 
Pfam
 PF00637; Clathrin
 PF09268; Clathrin-link
 PF01394; Clathrin_propel 
SMART
 SM00299; CLH 
PROSITE
 PS50236; CHCR 
PRINTS